Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Screening of conjugated linoleic acid (CLA) producing Lactobacillus plantarum and production of CLA on soy-powder milk by these stains

공액리놀레산 생성 Lactobacillus plantarum 선발 및 이를 이용한 콩-분말 두유에서 공액리놀레산 생산

  • Kim, Baolo (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Byong Won (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Hwang, Chung Eun (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Yu-Young (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Lee, Choonwo (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Kim, Byung Joo (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Park, Ji-Yong (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Sim, Eun-Yeong (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Haque, Md. Azizul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Dong Hoon (Department of Anatomy and Convergence Medical Science, School of Medicine, Gyeongsang National University) ;
  • Lee, Jin Hwan (Division of Research Development and Education, National Institute of Chemical Safety (NICS), Ministry of Environment) ;
  • Ahn, Min Ju (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Hee Yul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Ko, Jong Min (Department of South Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Kim, Hyun Tae (Department of South Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
  • Received : 2015.09.07
  • Accepted : 2015.09.24
  • Published : 2015.09.30
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Abstract

In this study, a total of 16 conjugated linoleic acid (CLA) producing lactic acid bacteria (LAB) were isolated from fermented foods. Among those strains, the S48 and P1201 strains were capable of producing higher CLA contents than other LABs. The two strains were classified as Lactobacillus plantarum based on morphological, physiological, chemotaxonomic, and molecular-genetic properties. The survival rates of these strain appeared to be 59.57% and 62.22% under artificial gastric conditions after 4 h at pH 2.5, respectively. These strains produced the cis-9, trans-11, and trans-10, cis-12 CLA isomers from 8% skim milk medium supplemented with the different free LA concentration at $37^{\circ}C$ for 48 h and the production of two CLA isomers constantly increased in the growth until 48 h of incubation. After 48 h of fermentation, the levels of CLA appeared highest in steamed soy-powder milk than fresh and roasted soy-powder milks. In particular, the CLA contents were produced $183.57{\mu}g/ml$ and $198.72{\mu}g/ml$ from steamed soy-powder milk after fermentation (48 h) with S48 and P1201 strains, respectively.

본 연구에서는 발효식품으로부터 16종의 공액리놀레산(CLA) 생성 젖산균을 분리하였다. 이들 균주 중, S48 및 P1201 균주는 다른 젖산균들보다 더 높은 CLA를 생성하였다. 두 균주는 형태학적, 생리학적, 화학적 및 분자유전학적 특징에 따라 Lactobacillus plantarum로 동정하였다. pH 2.0 인공위액산에서 4시간 배양 후 이들 균주의 생존율은 각각 59.57% 및 6.22%을 나타내었다. 이들 균주는 $37^{\circ}C$에서 48시간 동안 상이한 자유 리놀레산 함량이 함유되어 있는 8% 탈지분유 배지에서 cis-9, trans-11 및 trans-10, cis-12 CLA 이성체가 생성되었고, 두 CLA 이성체는 48시간 배양 동안 지속적으로 증가하였다. 발효 48시간 후, 증자콩-분말 두유의 CLA 함량은 생콩 및 볶음콩-분말 두유에서 보다 높은 생성능을 보였다. 특히, 증자콩-분말 두유의 CLA 함량은 각각 S48 및 P1201 균주에서 $183.57{\mu}g/ml$ 및 $198.72{\mu}g/ml$ 생성되었다.

Keywords

References

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