The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Antihyperglycemic α-Glucosidase Inhibitory Activity of Ethanol Extract from Neolentinus lepideus

잣버섯(Neolentinus lepideus) 에탄올 추출물의 혈당상승 억제 효과

  • Shin, Ja-Won (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Bae, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Yun-Hae (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Kim, Jeong-Han (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Ji, Jeong-Hyun (Mushroom Research Institute, Gyeonggi-do Agricultural Research & Extension Services) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 신자원 (배재대학교 바이오의생명공학과) ;
  • 배상민 (배재대학교 바이오의생명공학과) ;
  • 한상민 (배재대학교 바이오의생명공학과) ;
  • 이윤혜 (경기도 농업기술원 버섯연구소) ;
  • 김정한 (경기도 농업기술원 버섯연구소) ;
  • 지정현 (경기도 농업기술원 버섯연구소) ;
  • 이종수 (배재대학교 바이오의생명공학과)
  • Received : 2015.07.10
  • Accepted : 2015.09.11
  • Published : 2015.09.30
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Abstract

For development a new anti-diabetic compound from edible mushroom, antihyperglycemic ${\alpha}$-glucosidase inhibitory activities of Pleurotus ostreatus, Pleurotus cornucopiae, Pleurotus salmoneostramineus, Pleurotus eryngii and Neolentinus lepideus were investigated on its water and ethanol extracts. ${\alpha}$-Glucosidase inhibitory activity of Neolentinus lepideus fruiting body showed the highest at 86.3% in the 95% ethanol extracts and water extract from Pleurotus cornucopiae was also higher at 48.5% among water extracts. Therefore, Neolentinus lepideus which showed very high ${\alpha}$-glucosidase inhibitory activity was selected as a new anti-diabetic agent-containing mushroom and the ${\alpha}$-glucosidase inhibitor was maximally extracted when treated with 95% ethanol at $30^{\circ}C$ for 48 hr. The ethanol extracts from Neolentinus lepideus fruiting body showed dosage-dependent hypoglycemic action after administration to 120 min in the SD-rat and streptozotocin-induced diabetic SD-rat.

버섯을 이용하여 새로운 혈당상승 억제 물질을 개발하여 건강 기능성 식품 소재 산업에 응용하고자 느타리버섯, 노랑느타리버섯, 분홍느타리버섯, 새송이버섯과 잣버섯들의 ${\alpha}$-glucosidase 저해활성을 측정한 결과 잣버섯 자실체의 에탄올 추출물이 가장 높은 ${\alpha}$-glucosidase 저해활성을 보였다. 잣버섯 자실체 분말을 95% 에탄올로 $30^{\circ}C$에서 48시간 추출하였을 때 ${\alpha}$-glucosidase 저해물질이 가장 많이 추출되었고 이 ${\alpha}$-glucosidase 저해물질을 함유한 잣버섯 에탄올 추출물은 일반 쥐와 streptozotocin으로 당뇨를 유발시킨 당뇨 쥐에서 모두 농도 의존적으로 식후 혈당상승 억제 효과를 보였다.

Keywords

References

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