한국균학회지 (The Korean Journal of Mycology)

  • 제43권3호
  • /
  • Pages.149-157
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  • 2015
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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국내에 유통되는 종국 곰팡이의 분류학적 특성 및 안전성

Taxonomic Characterization and Safety of Nuruk Molds Used Industrially in Korea

  • 투고 : 2015.09.09
  • 심사 : 2015.09.22
  • 발행 : 2015.09.30
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초록

장류와 주류 제조를 위하여 국내에서 유통되는 하경발효의 황국균(HK1), 수원발효의 황국균(SW101), 백국균(SW201), 충무발효의 된장용 황국균(CF1001), 간장용 황국균(CF1002), 청주용 황국균(CF1003), 주류용 백국균(CF1005)과 전통 메주에서 분리하여 산업화 준비를 하고 있는 황국균(KACC 93210)의 분류학적 특성과 안전성을 조사하였다. 공시한 6균주의 황국균은 모두 A. oryzae로 동정되었으며 이중 HK1, SW101, CF1001, CF1003은 분생포자를 생성하는 대(stipe)의 길이가 중간크기($711{\sim}1,121{\mu}m$, 중모(中毛))로써 서로 유사한 형태적 및 분자계통학적 특성을 나타내었으나 청주용인 CF1003이 다른 3균주에 비하여 포자를 다소 적게 그리고 균사를 다소 많이 생성하였다. 간장제조용인 CF1002는 대의 길이가 평균 $543{\mu}m$로서 짧았으며(단모(短毛)) omtA 유전자 분석에서 다른 황국균이 ICAo 그룹에 위치하는 반면에 IBLB-그룹에 소속되어 명쾌히 구분되었다. 전통메주에서 분리되어 산업화 과정에 있는 황국균 KACC93210 균주는 대(stipe)가 평균 $270{\mu}m$로 매우 짧았으며(초단모(超短毛)) 다른 황국균이 양털모양의 집락을 형성하는 반면 벨벳모양의 집락을 형성하여 형태적으로 쉽게 구분되었고 omtA 유전자에서도 공시한 황국균은 물론 세계적으로 보고된 어떤 황국균과도 염기서열이 서로 달랐다. 수원발효의 백국균(SW201)과 충무발효의 백국균(CF1005)은 모두 A. luchuensis (또는 A. luchuensis mut. kawachii)로 동정되었으며 집락 형태가 다소 상이하였으나 기타의 형태적 특징과 분자계통학적 특징에서 서로 구분되지 않았다. 황국균 6균주는 aflatoxin, cyclopiazonic acid, sterigmatocystin의 곰팡이 독소를 생성하지 않았고 백국균(A. luchuensis)은 유해한 독소를 생성하지 않는 것으로 이미 보고되었으므로 국내에 유통되는 황국균과 백국균은 모두 곰팡이독소에서 안전한 곰팡이임이 입증되었다.

We examined taxonomic characteristics and safety of eight Nuruk molds that are widely used for making soybean paste, soy sauce and alcoholic beverages in Korea. HK1 from Hakyeong Fermentation Co., SW101 from Suwon Fermentation Co., CF1001, CF1002, CF1003 from Chungmoo Fermaentation Co. and KACC 93210 are yellow-Nuruk molds, and SW201 from Suwon Fermentation Co. and CF1005 from Chungmoo Fermentation Co. are white-Nuruk molds. Six strains of yellow-Nuruk molds were identified as Aspergillus oryzae. HK1, SW101, CF1001 and CF1003 of yellow-Nuruk molds have middle length of stipes ($711{\sim}1,121{\mu}m$), and CF1003 (for sake) produced less conidia and more hyphae than HK1, SW101 and CF1001 (for soybean paste). CF 1002 used for soy sauce has shorter stipes ($543{\mu}m$) and is clustered into IBLB-group based on omtA gene analysis although the other yellow-Nuruk molds are clustered into ICAo group. KACC 93210 isolated from traditional Korean Meju has very short stipes (average $270{\mu}m$), and showed velvety colonies although the others showed floccose colonies. The strain has different DNA sequences of omtA gene from other strains in NCBI GenBank as well as strains used in Korea, suggesting that it is unique from other strains published. SW201 and CF1005 of white-Nuruk molds were identified as Aspergillus luchuensis or A. luchuensis mut. Kawachii that is known as safe, non-toxigenic fungus. The six strains of yellow-Nuruk molds did not produce mycotoxins including aflatoxin, cyclopiazonic acid, and sterigmatocystin. Therefore, eight strains of Nuruk molds used for making soy sauce, soybean paste and alcoholic beverages in Korea were proved to be safe in this study.

키워드

참고문헌

  1. Hong SB, Yamada O, Samson RA. Taxonomic re-evaluation of black koji molds. Appl Microbiol Biotechnol 2014;98:555-61. https://doi.org/10.1007/s00253-013-5332-9
  2. Murakami H. Taxonomic studies on Japanese industrial strains of the Aspergillus (Part 32). J Brew Soc Jpn 1979;74:849-53. https://doi.org/10.6013/jbrewsocjapan1915.74.849
  3. Samson RA, Visagie CM, Houbraken J, Hong SB, Hubka V, Klaassen CH, Perrone G, Seifert KA, Susca A, Tanney JB, et al. Phylogeny, identification and nomenclature of the genus Aspergillus. Stud Mycol 2014;78:141-73. https://doi.org/10.1016/j.simyco.2014.07.004
  4. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. App Environ Microbiol 1995;61: 1323-30.
  5. Pildain MB, Frisvad JC, Vaamonde G, Cabral D, Varga J, Samson RA. Two novel aflatoxin-producing Aspergillus species from Argentinean peanuts. Int J Syst Evol Microbiol 2008;58:725-35. https://doi.org/10.1099/ijs.0.65123-0
  6. Hong SB, Lee M, Kim DH, Varga J, Frisvad JC, Perrone G, Gomi K, Yamada O, Machida M, Houbraken J, et al. Aspergillus luchuensis, an industrially important black Aspergillus in East Asia. PLoS One 2013;28:e63769.
  7. Hong SB, Lee M, Kim DH, Chung SH, Shin HD, Samson RA. The proportion of non-aflatoxigenic strains of the Aspergillus flavus/oryzae complex from Meju by analyses of the aflatoxin biosynthetic genes. J Microbiol 2013; 51:766-72. https://doi.org/10.1007/s12275-013-3128-3
  8. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  9. Tao L, Chung SH. Non-aflatoxigenicity of commercial Aspergillus oryzae strains due to genetic defects compared to aflatoxigenic Aspergillus flavus. J Microbiol Biotechnol 2014;24: 1081-7. https://doi.org/10.4014/jmb.1311.11011
  10. Hayashi Y, Takumi Y. Analysis of cyclopiazonic acid in corn and rice by newly developed method. Food Chem 2005;93: 215-21. https://doi.org/10.1016/j.foodchem.2004.09.017
  11. Rank C, Nielson KF, Larsen TO, Varga J, Samson RA, Frisvad JC. Distribution of sterigmatocystin in filamentous fungi. Fungal Biol 2011;115:406-20. https://doi.org/10.1016/j.funbio.2011.02.013
  12. Chang PK, Ehrlich KC, Hua SS. Cladal relatedness among Aspergillus oryzae isolates and Aspergillus flavus S and L morphotype isolates. Int J Food Microbiol 2006;108;172-7. https://doi.org/10.1016/j.ijfoodmicro.2005.11.008
  13. Kitahara K, Yoshida M. On the so-called Awamori white mold part III. (1) morphological and several physiological characteristics. J Ferment Technol 1949;27;162-6.