Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effect of Supplementation of Acanthopanax senticosus on Growth Performance, Blood Biochemical Profiles and Expression of Pro-Inflammatory Cytokines in Broiler Chicks

육계에서 가시오갈피 급여에 따른 생산성, 혈액 생화학적 성상 및 면역 사이토카인 발현에 미치는 영향

  • Jang, In-Surk (Dept. of Animal Science and Biotechnology, the Regional Animal Research Center (RAIC), Gyeongnam National University of Science and Technology) ;
  • Moon, Yang Soo (Dept. of Animal Science and Biotechnology, the Regional Animal Research Center (RAIC), Gyeongnam National University of Science and Technology) ;
  • Sohn, Sea Hwan (Dept. of Animal Science and Biotechnology, the Regional Animal Research Center (RAIC), Gyeongnam National University of Science and Technology)
  • 장인석 (경남과학기술대학교 동물생명과학과) ;
  • 문양수 (경남과학기술대학교 동물생명과학과) ;
  • 손시환 (경남과학기술대학교 동물생명과학과)
  • Received : 2015.08.10
  • Accepted : 2015.08.27
  • Published : 2015.09.30
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Abstract

This study was performed to examine the effects of dietary Acanthopanax senticosus (AS) on growth performance, immune organ weights, blood biochemical parameters and the expression of pro-inflammatory cytokines in broiler chicks. A total of 120 4-day-old birds were given a basal diet (CON) or a basal diet supplemented with 0.5% (AS1) or 1.0% (AS2) AS powder until the birds were 35 days of age. There was no difference in body weight, total gain, feed intake or immune organ weights among the treatment groups. However, the feed conversion ratio in the AS2 group was lower (p<0.05) than that in the CON group. Serum biochemical components, including AST (aspartate aminotransferase), ALT (alanine aminotransferase), albumin and total protein, were not affected by the dietary treatments, whereas glucose and triglyceride levels increased (p<0.05) in the AS2 group compared with the CON group. The AS1 group exhibited decreased mRNA expression (p<0.05) of IFN-${\gamma}$ in white blood cells and iNOS in the liver compared with the CON group. The other pro-inflammatory cytokines were unaffected by dietary AS supplementation, although there was a trend towards decreased expression of these genes, including those encoding Il-$1{\beta}$, IL-6 and TNF-${\alpha}$. In conclusion, dietary supplementation with 0.5% AS decreased the expression of several pro-inflammatory cytokines without affecting growth performance, suggesting that this supplement might be applicable as an immunoregulatory feed additive in broiler chicks.

본 연구는 천연항산화 생리활성 소재로서 가시오갈피를 육계에게 0(CON), 0.5(AS1) 및 1.0%(AS2) 수준으로 급여하여 생산성, 면역장기 무게, 혈액 생화학적 성상 및 친염증 사이토카인 mRNA 발현에 미치는 영향을 조사하였다. 전 사육기간에서 가시오갈피 급여에 따른 체중, 증체 및 사료섭취량은 유의적 차이를 보이지 않았으나, AS2구에서 사료요구율이 유의적으로(p<0.05) 저하되었다. 면역장기 무게를 관찰한 결과, 간, 비장, F-낭 및 흉선 무게는 가시오갈피 급여에 따른 영향이 없었다. 혈액 생화학적 성분에서 albumin, total protein, cholesterol, AST, ALT 등과 같은 대부분의 성분은 대조구와 처리구간 차이는 없었다. 그러나 triglycerides는 대조구에 비해 AS2구에서 현저히(p<0.05) 높았고, glucose 수준은 대조구에 비해 가시오갈피 급여구에서 현저히(p<0.05) 증가되었다. 친염증 사이토카인 mRNA 발현을 조사한 결과, 가시오갈피 급여에 따라 백혈구의 IFN-${\gamma}$ mRNA 발현이 현저히(p<0.05) 감소되었으며, IL1-${\beta}$, IL-6, TNF-${\alpha}$, iNOS 등은 유의적 차이는 없었지만, 감소되는 경향이 있었다. 비장에서 친염증 사이토카인 발현은 유의적 차이가 없었다. 간 조직에서는 0.5% 가시오갈피 급여에 따라 iNOS mRNA 발현이 유의적으로(p<0.05) 감소하였지만, 다른 친염증 사이토카인 발현은 유의적 차이는 없었다. 따라서 육계 생산성 및 친염증 사이토카인 발현에 미치는 영향 등을 고려할 경우, 0.5% 수준의 가시오갈피 급여가 가장 바람직한 것으로 판단된다.

Keywords

References

  1. Abou-Elkhair R, Ahmed HA, Selim S 2014 Effects of black pepper(Piper nigrum), turmeric powder(Curcuma longa) and coriander seeds(Coriandrum sativum) and their combinations as feed additives on growth performance, carcass traits, some blood parameters and humoral immune response of broiler chickens. Asian-Aust J Anim Sci. 27(6):847-584. https://doi.org/10.5713/ajas.2013.13644
  2. Al-Kassie GAM, Al-Nasrawi MAM, Ajeena SJ 2011 Use of black pepper(Piper nigrum) as feed additives in broilers diet. Opin Anim Vet Sci. 1:169-173.
  3. Chen R, Liu Z, Zhao J, Chen R, Meng F, Zhang M, Ge W 2011 Antioxidant and immunobiological activity of watersoluble polysaccharide fractions purified from Acanthopanax senticosu. Food Chem. 127:434-440. https://doi.org/10.1016/j.foodchem.2010.12.143
  4. Cho SK, Kim HI, Lee MK, Lee JJ, Kwak YC, Lee SC, Lee Y 2009 Effect of dietary supplementation of Glycyrrhizauralensis Fisch and Solanum nigrum L. mixture for alternate the antibiotics on productivity and blood composition in broiler chickens. Kor J Poult Sci. 36:215-222. https://doi.org/10.5536/KJPS.2009.36.3.215
  5. Davydov M, Krikorian AD 2000 Eleutherococcus senticosus (Rupr. & Maxim.) Maxim.(Araliaceae) as an adaptogen: A closer look. J Ethnopharmacol. 72:345-393. https://doi.org/10.1016/S0378-8741(00)00181-1
  6. Diallo A, Eklu-Gadegbeku K, Amegbor K, Agbonon A, Aklikokou K, Creppy E, Gbeassor M 2014 In vivo and in vitro toxicological evaluation of the hydroalcoholic leaf extract of Ageratum conyzoides L.(Asteraceae). J Ethnopharmacol. 155:1214-1218. https://doi.org/10.1016/j.jep.2014.07.005
  7. Gallois M, Rothkotter HJ, Bailey M, Stokes CR, Oswald IP 2009 Natural alternatives to in-feed antibiotics in pig production: Can immunomodulators play a role? Animal. 3: 1644-1661. https://doi.org/10.1017/S1751731109004236
  8. Giannini E, Botta F, Fasoli A, Ceppa P, Risso D, Lantieri PB, Celle G, Testa R 1999 Progressive liver functional impairment is associated with an increase in AST/ALT ratio. Dig Dis Sci. 44:1249-1253. https://doi.org/10.1023/A:1026609231094
  9. Guzik TJ, Korbut R, Adamek-Guzik T 2003 Nitric oxide and superoxide in inflammation and immune regulation. J Physiol Pharmacol. 54:469-487.
  10. Han J, Bian L, Liu X, Zhang F, Zhang Y, Yu N 2014 Effects of Acanthopanax senticosus polysaccharide supplementation on growth performance, immunity, blood parameters and expression of pro-inflammatory cytokines genes in challenged weaned piglets. Asian-Aust J Anim Sci. 27:1035-1043. https://doi.org/10.5713/ajas.2013.13659
  11. Huyghebaert G, Ducatelle R, Van Immerseel F 2011 An up date on alternatives to antimicrobial growth promoters for broilers. Vet J. 187:182-188. https://doi.org/10.1016/j.tvjl.2010.03.003
  12. Jang IS, Kang SY, Ko YH 2013 Influence of plum(Prunus mume Siebold and Zucc.) products on growth performance, intestinal function and immunity in broiler chicks. J Poult Sci. 50:28-36. https://doi.org/10.2141/jpsa.0120028
  13. Jin LH, Han SS, Choi YS 2002 Antioxidant effects of the extracts of Acanthopanax senticosus. Kor J Pharmacogn. 33 (4):359-363.
  14. Kang HK, Beloor J, Sohn SH, Jang IS, Moon YS 2009 Effect of dietary supplementation of Acanthopanax senticosus and Eucommiaceae on the expression of lipogenic, myogenic and antioxidant enzyme genes in broiler chickens. Kor J Poult Sci. 36:39-45. https://doi.org/10.5536/KJPS.2009.36.1.039
  15. Ko YH, Lee SS, Jang IS 2010 Effects of dietary supplementation of ginkgo leaf and pumpkin on growth performance, intestinal microflora, blood biochemical profile and antioxidant status in broiler chickens. Kor J Poult Sci. 37: 23-33. https://doi.org/10.5536/KJPS.2010.37.1.023
  16. Ko YH, Moon YS, Sohn SH, Jung CY, Jang IS 2012 Effect of dietary supplementation of plum or omija on growth performance, blood biochemical profiles and antioxidant defense system in broiler chickens Kor J Poult Sci. 39:121-131. https://doi.org/10.5536/KJPS.2012.39.2.121
  17. Koo BK, Chung JM, Lee HS 1998 Biochemical evaluation of nutritional status of protein and lipid in patients with alcoholic liver disease. Kor J Food Sci Nutr. 27:1236-1243.
  18. Korver DR 2012 Implications of changing immune function through nutrition in poultry. Anim Feed Sci & Tech. 173: 54-64. https://doi.org/10.1016/j.anifeedsci.2011.12.019
  19. Lee S, Son D, Ryu J, Lee YS, Jung SH, Kang J, Lee SY, Kim HS, Shin KH 2004 Anti-oxidant activities of Acanthopanax senticosus stems and their lignan components. Arch Pharm Res. 27:106-110. https://doi.org/10.1007/BF02980055
  20. Li WG, Zhang XY, Wu YJ, Tian X 2001 Anti-inflammatory effect and mechanism of proanthocyanidins from grape seeds. Acta Pharmacol Sin. 22:1117-1120.
  21. Lin QY, Jin LJ, Cao ZH, Lu YN, Xue HY, Xu YP 2008 Athopanax senticosus suppresses reactive oxygen species production by mouse peritoneal macrophages in vitro and in vivo. Phytother Res. 22:740-745. https://doi.org/10.1002/ptr.2341
  22. Lin QY, Jin LJ, Ma YS, Shi M, Xu YP 2007 Acanthopanax senticosus inhibits nitric oxide production in murine macrophages in vitro and in vivo. Phytother Res. 21:879-883. https://doi.org/10.1002/ptr.2171
  23. Livak KJ, Schmittgen TD 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) Method. Methods. 25:402-408. https://doi.org/10.1006/meth.2001.1262
  24. Macdonald J, Galley HF, Webster NR. 2003 Oxidative stress and gene expression in sepsis. Br J Anaesth 90:221-232. https://doi.org/10.1093/bja/aeg034
  25. Novoselova EG, Lunin SM, Novoselova TV, Khrenov MO, Glushkova OV, Avkhacheva NV, Safronova VG, Fesenko EE. 2009 Naturally occurring antioxidant nutrients reduce inflammatory response in mice. Eur J Pharmacol. 615:234-240. https://doi.org/10.1016/j.ejphar.2009.05.004
  26. Schmolz MW, Sacher F, Aicher B 2001 The synthesis of Rantes, G-CSF, IL-4, IL-5, IL-6, IL-12 and IL-13 in human whole-blood cultures is modulated by an extract from Eleutherococcus senticosus L. roots. Phytother Res. 15:268-70. https://doi.org/10.1002/ptr.746
  27. Wang X, Hai CX, Liang X, Yu SX, Zhang W, Li YL 2010 The protective effects of Acanthopanax senticosus Harms aqueous extracts against oxidative stress: Role of Nrf2 and antioxidant enzymes. J Ethnopharmacol. 127:424-432. https://doi.org/10.1016/j.jep.2009.10.022
  28. Wenk C 2000 Recent advances in animal feed additives such as metabolic modifiers, antimicrobial agents, probiotics, enzymes and highly available minerals. Review. Asian-Aust J Anim Sci. 13:86-95. https://doi.org/10.5713/ajas.2000.86
  29. Yoon TJ, Yoo YC, Lee SW, Shin KS, Choi WH, Hwang SH, Ha ES, Jo SK, Kim SH, Park WM 2004 Anti-metastatic activity of Acanthopanax senticosus extract and its possible immunological mechanism of action. J Ethnopharmacol. 93:247-253. https://doi.org/10.1016/j.jep.2004.03.052
  30. Zuidhof MJ, Schneider BL, Carney VL, Korver DR, Robinson FE 2014 Growth, efficiency, and yield of commercial broilers from 1957, 1978, and 2005. Poult Sci. 93:2970-2982. https://doi.org/10.3382/ps.2014-04291