Applied Science and Convergence Technology

The Korean Vacuum Society (한국진공학회)
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A Reusable Pb2+ Detecting Aptasensor Employing a Gold Nanorod-DNAzyme Conjugate

  • Lee, Jayeon (Nanobiotechnology (Major), KRIBB Campus, Korea University of Science and Technology) ;
  • Ha, Tai Hwan (Nanobiotechnology (Major), KRIBB Campus, Korea University of Science and Technology)
  • Received : 2015.09.02
  • Accepted : 2015.09.16
  • Published : 2015.09.30
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Abstract

Here, we demonstrated a $Pb^{2+}$ detecting aptasensor using $Pb^{2+}$-sensitive DNAzyme-conjugated gold nanorods (GNRs). Fluorescent DNA substrates that were initially quenched by GNRs, are released in response to $Pb^{2+}$ ions to give a substantial fluorescence signal. The GNR-tethered DNAzyme is reusable at least three times with a LOD of 50 nM.

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References

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