Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of a Rapid and Accurate Identification Method for Citrobacter Species Isolated from Pork Products Using a Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDITOF MS)

  • Kwak, Hye-Lim (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Han, Sun-Kyung (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Park, Sunghoon (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Park, Si Hong (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Shim, Jae-Yong (Department of Food & Biotechnology, Hankyong National University) ;
  • Oh, Mihwa (National Institute of Animal Science, Rural Development Administration) ;
  • Ricke, Steven C. (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University) ;
  • Kim, Hae-Yeong (Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University)
  • Received : 2015.03.20
  • Accepted : 2015.05.24
  • Published : 2015.09.28
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Abstract

Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.

Keywords

References

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