International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지
DOI QR코드

DOI QR Code

Gene Expression Profiling by Microarray during Tooth Development of Rats

  • Yoo, Hong-Il (Dental Science Research Institute, School of Dentistry, Chonnam National University) ;
  • Shim, Hae-Kyoung (Dental Science Research Institute, School of Dentistry, Chonnam National University) ;
  • Kim, Sun-Hun (Dental Science Research Institute, School of Dentistry, Chonnam National University)
  • Received : 2015.09.11
  • Accepted : 2015.09.18
  • Published : 2015.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Odontogenic cells express many genes spatiotemporally through complex and intricate processes during tooth formation. Therefore, investigating them during the tooth development has been an important subject for the better understanding of tooth morphogenesis. The present study was performed to identify the genetic profiles which are involved in the morphological changes during the different stages of rat tooth development using the Agilent Rat Oligonucleotide Microarrays. Morphologically, the maxillary 3rd molar germ at 10 days post-partum (dpp) was at the cap/bell stage. In contrast, the maxillary 2nd molar germ showed the root development stage. After microarray analysis, there were a considerable number of up- or down-regulated genes in the 3rd and the 2nd molar germ cells during tooth morphogenesis. Several differentially expressed genes for nerve supply were further studied. Among them, neuroligin 1 (Nlgn 1) was gradually downregulated during tooth development both at the transcription and the translation level. Also, Nlgn 1 was mostly localized in the dental sac, which is an important component yielding the nerve supply. This genetic profiling study proposed that many genes may be implicated in the biological processes for the dental hard tissue formation and, furthermore, may allow the identification of the key genes involved in the nerve supply to the dental sac.

Keywords

References

  1. Kollar EJ, Mina M. Role of the early epithelium in the patterning of the teeth and Meckel's cartilage. J Craniofac Genet Dev Biol. 1991;11:223-228.
  2. Slavkin HC Molecular determinants during dental morphogenesis and cytodifferentiation: a review. J Craniofac Genet Dev Biol. 1991;11:338-349.
  3. Thesleff I. Epithelial-mesenchymal signaling regulating tooth morphogenesis. J Cell Sci. 2003;116:1647-1648. https://doi.org/10.1242/jcs.00410
  4. Pispa J, Thesleff I. Mechanisms of ectodermal organogenesis. Dev Biol. 2003;262:195-205. https://doi.org/10.1016/S0012-1606(03)00325-7
  5. Fincham AG, Moradian-oldak J, Simmer JP. The structural biology of the developing dental enamel matrix. J Struct Biol. 1999;126:270-299. https://doi.org/10.1006/jsbi.1999.4130
  6. Thesleff I, Nieminen P. Tooth morphogenesis and cell differentiation. Curr Opin Cell Biol. 1996;8:844-850. https://doi.org/10.1016/S0955-0674(96)80086-X
  7. Thesleff I, Vaahtokari A, Vainio S, Jowett A. Molecular mechanisms of cell and tissue interactions during early tooth development. Anat Rec. 1996;245:151-161. https://doi.org/10.1002/(SICI)1097-0185(199606)245:2<151::AID-AR4>3.0.CO;2-#
  8. Schena M, Shalon D, Davis RW, Brown PO. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 1995;270:467-470. https://doi.org/10.1126/science.270.5235.467
  9. Berger JA, Hautaniemi S, Järvinen AK, Edgren H, Mitra SK, Astola J. Optimized LOWESS normalization parameter selection for DNA microarray data. BMC Bioinformatics 2004;5:194. doi:10.1186/1471-2105-5-194.
  10. Dennis G Jr, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA. DAVID: Database for Annotation, Visualization, and Integrated Discovery. Genome Biol. 2003;4:P3. https://doi.org/10.1186/gb-2003-4-5-p3
  11. Luukko K, Moe K, Sijaona A, Furmanek T, Hals Kvinnsland I, Midtbo M, Kettunen P. Secondary induction and the development of tooth nerve supply. Ann Anat. 2008;190:178-187. https://doi.org/10.1016/j.aanat.2007.10.003
  12. Luukko K, Kettunen P. Coordination of tooth morphogenesis and neuronal development through tissue interactions: lessons from mouse models. Exp Cell Res. 2014;325:72-77. https://doi.org/10.1016/j.yexcr.2014.02.029
  13. Fledrich R, Stassart RM, Klink A, Rasch LM, Prukop T, Haag L, Czesnik D, Kungl T, Abdelaal TA, Keric N, Stadelmann C, Bruck W, Nave KA, Sereda MW. Soluble neuregulin-1 modulates disease pathogenesis in rodent models of Charcot-Marie-Tooth disease 1A. Nat Med. 2014;20:1055-1061. doi: 10.1038/nm.3664.
  14. Verhamme C, King RH, ten Asbroek AL, Muddle JR, Nourallah M, Wolterman R, Baas F, van Schaik IN. Myelin and axon pathology in a long-term study of PMP22-overexpressing mice. J Neuropathol Exp Neurol. 2011;70:386-398. doi: 10.1097/NEN.0b013e318217eba0.
  15. Raphael AR, Talbot WS. New insights into signaling during myelination in zebrafish. Curr Top Dev Biol. 2011;97:1-19. doi: 10.1016/B978-0-12-385975-4.00007-3.
  16. Ichtchenko K, Hata Y, Nguyen T, Ullrich B, Missler M, Moomaw C, Sudhof TC. Neuroligin 1: a splice site-specific ligand for beta-neurexins. Cell 1995;81:435-443. https://doi.org/10.1016/0092-8674(95)90396-8
  17. Ichtchenko K, Nguyen T, Südhof TC. Structures, alternative splicing, and neurexin binding of multiple neuroligins. J Biol Chem. 1996;271:2676-2682. https://doi.org/10.1074/jbc.271.5.2676
  18. Irie M, Hata Y, Takeuchi M, Ichtchenko K, Toyoda A, Hirao K, Takai Y, Rosahl TW, Südhof TC. Binding of neuroligins to PSD-95. Science 1997;277:1511-1515. https://doi.org/10.1126/science.277.5331.1511
  19. Ushkaryov YA, Petrenko AG, Geppert M, Südhof TC. Neurexins: synaptic cell surface proteins related to the alphalatrotoxin receptor and laminin. Science. 1992;257:50-56. https://doi.org/10.1126/science.1621094
  20. Ullrich B, Ushkaryov YA, Sudhof TC. Cartography of neurexins: more than 1000 isoforms generated by alternative splicing and expressed in distinct subsets of neurons. Neuron 1995;14:497-507. https://doi.org/10.1016/0896-6273(95)90306-2