International Journal of Oral Biology

  • 제40권3호
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  • Pages.111-116
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  • 2015
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Root Resorption in Streptozotocin-induced Diabetic Rats with Ligature-induced Periodontitis

  • Kim, Ji-Hye (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • Lee, Dong-Eun (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • Park, Jung-Chul (Department of Periodontology, Dankook University College of Dentistry) ;
  • Kim, Yoon Jae (Yonsei University College of Dentistry) ;
  • Cha, Jeong-Heon (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • Bak, Eun-Jung (Department of Oral Biology, Yonsei University College of Dentistry) ;
  • Yoo, Yun-Jung (Department of Oral Biology, Yonsei University College of Dentistry)
  • 투고 : 2015.07.22
  • 심사 : 2015.09.01
  • 발행 : 2015.09.30
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초록

To determine the effect of diabetes on root resorption in periodontitis, we investigated odontoclast formation and root resorption in diabetic rats with periodontitis. Odontoclast formation was observed in three groups of F344 rats: Controls (C) were normal rats without diabetes or periodontitis; the periodontitis (P) group had mandibular first molars to be ligatured; the periodontitis with diabetes (PD) group was intravenously administered streptozotocin (50 mg/kg) to induce diabetes and had mandibular first molars to be ligatured. On days 3, 10, and 20 after ligature, tumor necrosis factor (TNF)-${\alpha}$ and receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL) expression, odontoclast formation, and root resorption areas were evaluated by immunohistochemistry, tartrate-resistant acid phosphatase staining, and hematoxylin and eosin staining, respectively. The PD group showed frequent urination, weight loss, and hyperglycemia. Numbers of TNF-${\alpha}$- and RANKL-positive cells were higher in the P and PD groups than in the C group. It was more prevalent in PD group on day 3. Odontoclast formation was greater in the P and PD groups than in the C group on days 3 and 10, then decreased to same level as the C group by day 20. Root resorption in the PD and P groups showed increases on days 3 and 10, respectively, compared to the C group. These results suggest that diabetes may transiently increase root resorption on day 3 with high expression of TNF-${\alpha}$ and RANKL after periodontitis induction. This study could aid the understanding of root resorption in diabetic patients with periodontitis.

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참고문헌

  1. Fuss Z, Tsesis I, Lin S. Root resorption-diagnosis, classification and treatment choices based on stimulation factors. Dent Traumatol. 2003;19:175-182. https://doi.org/10.1034/j.1600-9657.2003.00192.x
  2. Harokopakis-Hajishengallis E. Physiologic root resorption in primary teeth: molecular and histological events. J Oral Sci. 2007;49:1-12. https://doi.org/10.2334/josnusd.49.1
  3. Crespo Abelleira AC, Rodriguez Cobos MA, Fuentes Boquete IM, Castano Oreja MT, Jorge Barreiro FJ, Rodriguez Pato RB. Morphological study of root surfaces in teeth with adult periodontitis. J Periodontol. 1999;70:1283-1291. https://doi.org/10.1902/jop.1999.70.11.1283
  4. Harvey BL, Zander HA. Root surface resorption of periodontally diseased teeth. Oral Surg Oral Med Oral Pathol. 1959;12:1439-1443. https://doi.org/10.1016/0030-4220(59)90264-6
  5. Rodriguez-Pato RB. Root resorption in chronic periodontitis: a morphometrical study. J Periodontol. 2004;75:1027-1032. https://doi.org/10.1902/jop.2004.75.7.1027
  6. Domon T, Osanai M, Yasuda M, Seki E, Takahashi S, Yamamoto T, Wakita M. Mononuclear odontoclast participation in tooth resorption: the distribution of nuclei in human odontoclasts. Anat Rec. 1997;249:449-457. https://doi.org/10.1002/(SICI)1097-0185(199712)249:4<449::AID-AR4>3.0.CO;2-M
  7. Sahara N, Toyoki A, Ashizawa Y, Deguchi T, Suzuki K. Cytodifferentiation of the odontoclast prior to the shedding of human deciduous teeth: an ultrastructural and cytochemical study. Anat Rec. 1996;244:33-49. https://doi.org/10.1002/(SICI)1097-0185(199601)244:1<33::AID-AR4>3.0.CO;2-G
  8. Tomokazu Y, Masaru Y, Mami S, Kunihiko Y, Kazutaka K. TNF-${\alpha}$ aggravates the progression of orthodonticallyinduced inflammatory root resorption in the presence of RANKL. J Hard Tissue Biol. 2014;23:155-1562. 10.2485/jhtb.23.155.
  9. Lamster IB, Lalla E, Borgnakke WS, Taylor GW. The relationship between oral health and diabetes mellitus. J Am Dent Assoc. 2008;139 Suppl:19S-24S.
  10. Kim JH, Lee DE, Gunawardhana KS, Choi SH, Woo GH, Cha JH, Bak EJ, Yoo YJ. Effect of the interaction between periodontitis and type 1 diabetes mellitus on alveolar bone, mandibular condyle and tibia. Acta Odontol Scand. 2014;72:265-273. doi: 10.3109/00016357.2013.822551.
  11. Jiang ZL, Cui YQ, Gao R, Li Y, Fu ZC, Zhang B, Guan CC. Study of TNF-${\alpha}$, IL-$1{\beta}$ and LPS levels in the gingival crevicular fluid of a rat model of diabetes mellitus and periodontitis. Dis Markers. 2013;34:295-304. doi: 10.3233/DMA-130974.
  12. Pacios S, Kang J, Galicia J, Gluck K, Patel H, Ovaydi-Mandel A, Petrov S, Alawi F, Graves DT. Diabetes aggravates periodontitis by limiting repair through enhanced inflammation. FASEB J. 2012;26:1423-1430. doi: 10.1096/fj.11-196279.
  13. Kim JH, Lee DE, Woo GH, Cha JH, Bak EJ, Yoo YJ. Osteocytic sclerostin expression in alveolar bone in diabetic rats with ligature induced-periodontitis. J Periodontol. 2015.86:1005-1011. doi:10.1902/jop.2015.150083.1-14.
  14. Kim JH, Lee DE, Cha JH, Bak EJ, Yoo YJ. Receptor activator of nuclear factor-${\kappa}B$ ligand and sclerostin expression in osteocytes of alveolar bone in rats with ligature-induced periodontitis. J Periodontol. 2014;85:e370-e378. doi: 10.1902/jop.2014.140230.
  15. Zhou T, Chen D, Li Q, Sun X, Song Y, Wang C. Curcumin inhibits inflammatory response and bone loss during experimental periodontitis in rats. Acta Odontol Scand. 2013;71:349-356. doi: 10.3109/00016357.2012.682092.
  16. Yang X, Zhang H, Wang J, Zhang Z, Li C. Puerarin decreases bone loss and collagen destruction in rats with ligatureinduced periodontitis. J Periodontal Res. 2015. doi:10.1111/jre.12261.
  17. Santos VR, Lima JA, Goncalves TE, Bastos MF, Figueiredo LC, Shibli JA, Duarte PM. Receptor activator of nuclear factor-kappa B ligand/osteoprotegerin ratio in sites of chronic periodontitis of subjects with poorly and well-controlled type 2 diabetes. J Periodontol. 2010;81:1455-1465. doi: 10.1902/jop.2010.100125.
  18. Tadashi K, Masaru Y, Tomokazu Y, Mami S, Kunihiko Y, Takemi G. Kazutaka K. TNF-${\alpha}$ and RANKL facilitates the development of orthodontically-induced inflammatory root. Open Journal of Stomatology. 2013;3:52-58. doi: 10.4236/ojst.2013.39A008.
  19. Yongchaitrakul T, Lertsirirangson K, Pavasant P. Human periodontal ligament cells secrete macrophage colonystimulating factor in response to tumor necrosis factor-alpha in vitro. J Periodontol. 2006;77:955-962. https://doi.org/10.1902/jop.2006.050338
  20. Suda T, Takahashi N, Udagawa N, Jimi E, Gillespie MT, Martin TJ. Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr Rev. 1999;20:345-357. https://doi.org/10.1210/edrv.20.3.0367
  21. Kohsaka T, Kumazawa M, Yamasaki M, Nakamura H. Periapical lesions in rats with streptozotocin-induced diabetes. J Endod. 1996;22:418-421. https://doi.org/10.1016/S0099-2399(96)80243-3