Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effect of Lactic Acid Bacteria Inoculation on Fermentation Characteristics of Whole Crop Barley Silage

  • Received : 2015.06.06
  • Accepted : 2015.08.22
  • Published : 2015.10.01
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Abstract

An experiment was carried out to determine the homofermentative activity of Lactobacillus plantarum KCC-10 and KCC-19 on the ensiling of whole crop barley (WCB). The crude protein in the silages was slightly higher in the KCC-10 and KCC-19 treatments compared to the control, but there was no significant difference between the two inoculant-treated silages. Nutrient parameters such as acid detergent fiber, neutral detergent fiber and in vitro dry matter digestibility in L. plantarum KCC-10 and KCC-19 treated silages did not differ from those in the control silage. The lactic acid content increased in KCC-10 and KCC-19 treated silage when compared with the control silage but the contents of acetic acid and butyric acid produced in KCC-10 and KCC-19 treated silages were similar with the control silage. Further, the number of lactic acid bacteria (LAB) in KCC-10 treated silage demonstrated a significant increase when compared to the control. Especially, KCC-19 treated silage showed greater lactic acid bacterial growth potential. Other microbes such as yeast and fungi were not detected in KCC-10 and KCC-19 treated WCB silages. Hence, this study suggests that the addition of L. Plantarum KCC-10 and KCC-19 to the WCB silage can improve fermentation quality for the production of high-quality silage.

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References

  1. Acosta Aragon, Y., Jatkauskas, J. and Vrotniakiene, V. 2012. The Effect of a Silage Inoculant on Silage Quality, Aerobic Stability, and Meat Production on Farm Scale. International Scholary Research network. 12:1-6.
  2. AOAC. 1990. Official method of analysis. 15thed. Washington, DC.
  3. Avila, C.L.S., Pinto, J.C., Figueiredoh, C.P. and Schwan, R.F. 2009. Effects of an indigenous and a commercial Lactobacillus buchneri strain on quality of sugar cane silage. Grass and Forage Science. 64:384-394. https://doi.org/10.1111/j.1365-2494.2009.00703.x
  4. Avila, C.L.S., Pinto, J.C., Oliveira, D.P. and Schwan, R.F. 2012. Aerobic stability of sugar cane silages with a novel strain of Lactobacillus sp. isolated from sugar cane. Brazilian Journal Animal Science. 41:249-255.
  5. Borreani, G. and Tabacco, E. 2010. The relationship of silage temperature with the microbiological status of the face of corn silage bunkers. Journal of Dairy Science. 93:2620-2629. https://doi.org/10.3168/jds.2009-2919
  6. Bucher, E. 1970. Beitrage zur Mikrobiologie der Silagegarung und der Garfutterstabilitat. Ph.D. thesis. Ludwig Maximilans University, Munich, Germany.
  7. Cai, Y., Benno, Y., Ogawa, M. and Kumai, S. 1999. Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage. Journal of Dairy Science. 82:520-526. https://doi.org/10.3168/jds.S0022-0302(99)75263-X
  8. Cao, Y., Cai, Y., Takahashi, T., Yoshida, N., Tohno, M., Uegaki, R., Nonaka, K. and Treada, F. 2011. Effect of lactic acid bacteria inoculant and beet pulp addition on fermentation characteristics and in vitro ruminal digestion of vegetable residue silage. Journal of Dairy Science. 94:3902-3912. https://doi.org/10.3168/jds.2010-3623
  9. Cao, Y., Takahashi, T., Horiguchi, K. and Yoshida, N. 2010. Effect of adding lactic acid bacteria and molasses on fermentation quality and in vitro ruminal digestion of total mixed ration silage prepared with whole crop rice. Grassland Science. 56:19-25. https://doi.org/10.1111/j.1744-697X.2009.00168.x
  10. Carvalho, B.F., Avila, C.L.S., Miguel, M.G.C.P., Pinto, J.C., Santos, M.C. and Schwan, R.F. 2014. Aerobic stability of sugar-cane silage inoculated with tropical strains of lactic acid bacteria. Grass and Forage Science. 70:308-323.
  11. Choi, K.C., Jo, N.C., Jung, M.W., Lee, K.D., Kim, J.G., Lim, Y.C., Kim, W.H., Oh, Y.K., Choi, J.H., Kim, C.M., Jung, D.K., Choi, J.M. and Kim, H.G. 2011a. Effect of harvest stage of corn on nutritive values and quality of roll baled corn silage manufactured with corn grown in paddy land. Journal of the Society of Grassland and Forage Science. 31:65-74. https://doi.org/10.5333/KGFS.2011.31.1.65
  12. Choi, K.C., Jung, M.W., Kim, W.H., Kim, C.M., Yoon, S.H., Choi, E.M., Kim, J.G., Lee, S.M., Choi, J.M, Kim, H.G. and Lim, Y.C. 2011b. Effect of harvest Stage of $Sorghum{\times}Sorghum$ Hybrid (SSH) on the quality of round baled SSH silage. Journal of the Society of Grassland and Forage Science. 31:143-150. https://doi.org/10.5333/KGFS.2011.31.2.143
  13. Cooke, L. 1995. New strains slow silage spoilage. Agricultural Research. 40:17.
  14. Dannerh, H., Holzer, M., Mayrhuber, E. and Braun, R. 2003. Acetic acid increases stability of silage under aerobic conditions. Applied and Environmental Microbiology. 69:562-567. https://doi.org/10.1128/AEM.69.1.562-567.2003
  15. Holland, C., Kezar W., Kautz, W.P., Lazowski, E.J., Mahanna W.C. and Reinhart, R. 1990. Pioneer forage manual: A nutritional guide. Pioneer Hi-Bred International, Inc. Des moines, IA. pp. 1-55.
  16. Holzer, M. 2001. Development of a novel silage starter for the improvement of aerobic stability and quality with special focus on Lactobacillus buchneri. Ph.D. thesis. University of Agricultural Sciences, Vienna, Austria.
  17. Honig, H. 1990. Evaluation of aerobic stability. Grass Forage Rep. Spec. Issue 3:76-82.
  18. Hristov, A.N., McAllister, T.A. and Graham. S. 2000. Effect of inoculants on whole-crop barley silage fermentation and degradability of dry matter in the rumen. Proceedings, Western Section, American Society of Animal Science. 51:433-436.
  19. Hristov, A.N. and McAllister, T.A. 2002. Effect of inoculants on whole-crop barley silage fermentation and dry matter disappearance in situ. Journal of Animal Science. 80:510-516. https://doi.org/10.2527/2002.802510x
  20. Ilavenil, S., Arasu, M.V., Vijayakumar, M., Jung, M.W., Park, H.S., Lim, Y.C. and Choi, K.C. 2014. Lactobacillus plantarum Improves the Nutritional Quality of Italian Ryegrass with Alfalfa Mediated Silage. Journal of the Korean Society of Grassland and Forage Science. 34(3):174-178. https://doi.org/10.5333/KGFS.2014.34.3.174
  21. Kristensen, N.B., Storm, A., Raun, B.M.L., Rojen, B.A. and Harmon, D.L. 2007. Metabolism of silage alcohols in lactating dairy cows. Journal of Dairy Science. 90:1364-1377. https://doi.org/10.3168/jds.S0022-0302(07)71623-5
  22. Miller, T.L. and Wolin, M.J. 1974. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Applied Microbiology. 27:985-987.
  23. Moon, N.J. 1983. Inhibition of the growth of acid tolerant yeasts by acetate, lactate and their synergistic mixtures. Journal of Applied Bcteriology. 55:453-460. https://doi.org/10.1111/j.1365-2672.1983.tb01685.x
  24. Moon, N.J., Ely, L.O. and Sudweeks, E.M. 1980. Aerobic deterioration of wheat, lucerne and maize silages prepared with Lactobacillus acidophilus and a Candida spp. Journal of Applied Bacteriology. 49:75-87. https://doi.org/10.1111/j.1365-2672.1980.tb01045.x
  25. Moore, J.E. 1970. Procedure for the two-stage in vitro digestion of forage. University of Florida, Department of Animal Science.
  26. Oude Elferink, S.J.W.H., Krooneman, J., Gottschal, J.A., Spoelstra, S.F. and Faber, F. 2001. Anaerobic conversion of lactic acid to acetic acid and 1,2-propanediol by Lactobacillus buchneri. Applied and Environmental Microbiology. 67:125-132. https://doi.org/10.1128/AEM.67.1.125-132.2001
  27. Pirt, S.J. 1975. Principles of microbe and cell cultivation. Blackwell Scientific Publications, Oxford, United Kingdom.
  28. Seo, S., Kim, W.H., Kim, J.G., Choi, G.J., Kim, K.Y., Cho, W.M., Park, B.Y. and Kim, Y.H. 2010. Effect of Whole Crop Barley Silage Feeding on the Growth Performance, Feed Requirement and Meat Quality of Hanwoo Steers. Journal of the Korean Society of Grassland and Forage Science. 30(3):257-266. https://doi.org/10.5333/KGFS.2010.30.3.257
  29. Valan Arasu M, Jung, M.W., Ilavenil S, Jane, M. Kim, D.H., Lee, K.D., Park, H.S., Huh, T.Y., Choi, G.J., Lim, Y.C., Al-Dhabi N.A. and Choi, K.C. 2013. Isolation and characterization of antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties. Journal of Applied Microbiology. 115(5): 1172-85. https://doi.org/10.1111/jam.12319
  30. Valan Arasu M, Jung, M.W., Kim, D.H., Ilavenil S., Lee, K.D., Choi, G.J., Al-Dhabi N.A. and Choi, K.C. 2014. Isolation and characterization of Lactobacillus plantarum KCC-19 from crimson silage. Journal of Pure and Applied Microbiology. 8(5):3575-3587.
  31. Van Soest, P.J. In H.G., Jung, D.R., Buxton, R.D. and Ralph eds, J. 1993. Cell wall matrix interactions and degradation session synopsis. Forage Cell Wall Structure and Digestibility. American Society. Agronomy Madison, WI. 377-395.
  32. Weinberg, Z.G. and Muck, R.E. 1996. New trends and opportunities in the development and use of inoculants for silage. FEMS Microbiology Reviews. 19:53-68.
  33. Weinberg, Z.G., Ashbell, G., Hen, Y. and Azrieli, A. 1993. The effect of applying lactic acid bacteria at ensiling on the aerobic stability of silages. Journal of Applied Bacteriology. 75:512-518. https://doi.org/10.1111/j.1365-2672.1993.tb01588.x