Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Inhibitory Effects of Soyasaponins on Antigen-induced Degranulation in RBL-2H3 Cells

Soyasaponin의 RBL-2H3 세포에서 탈과립화 억제 효과

  • Yang, Seung Hwan (Center for Nutraceutical and Pharmaceutical Materials, Myongji University) ;
  • Lee, Jung A (Bio-center, Gyeonggi Institute of Science and Technology Promotion) ;
  • Lee, Jae Yeon (Bio-center, Gyeonggi Institute of Science and Technology Promotion) ;
  • Ahn, Eun-Kyung (Bio-center, Gyeonggi Institute of Science and Technology Promotion) ;
  • Shin, Tai-Sun (Division of Food & Nutrition, Chonnam National University) ;
  • Tsukamoto, Chigen (Department of Applied Biological Chemistry, Iwate University) ;
  • Chung, Gyuhwa (Department of Biotechnology, Chonnam National University) ;
  • Suh, Joo-Won (Center for Nutraceutical and Pharmaceutical Materials, Myongji University)
  • Received : 2015.07.01
  • Accepted : 2015.08.05
  • Published : 2015.09.30
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Abstract

Soyasaponins are glycosylated, which gives rise to a wide diversity of structures and functions. We evaluated for inhibitory effects of 4 soyasaponins on antigen-induced degranulation in RBL-2H3 Cells. 4 soyasaponins had shown dose-dependently inhibited histamine and ${\beta}$-hexosaminidase.

본 연구에서는 콩의 배축(hypocotyls)에서 분리된 4종의 soyasaponin(Aa, Ab, bg, DDMP)에 대해 RBL-2H3 세포를 이용하여 항알레르기 활성을 확인하고자 하였다. RBL-2H3 세포에서 soyasaponin 화합물들의 세포독성을 확인해 본 결과, 4종 모두 세포독성이 없었고 ${\beta}$-hexosaminidase assay를 통해 비만세포의 탈과립 억제 효능을 확인해 본 결과 대조군으로 사용한 Ketotifen fumarate($IC_{50}$: 38.77mM)와 비교하여 4종 모두 ${\beta}$-hexosaminidase 억제 효능이 있는 것을 확인하였다. 또한, soyasaponin 화합물들은 RBL-2H3 세포에서 탈과립 후 유리되는 대표적인 물질인 histamine의 분비 억제에도 효능이 있음을 확인하였고 이 중 Soyasaponin DDMP가 가장 효능이 우수한 것으로 판단된다.

Keywords

References

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