Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Identification of Triterpenoids and Sterols from the Flowers of Chionanthus retusus Lindl. & Paxton

이팝나무(Chionanthus retusus Lindl. & Paxton) 꽃으로부터 Triterpenoid 및 Sterol 화합물의 분리 및 동정

  • Jung, Jae-Woo (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Seo, Kyeong-Hwa (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Oh, Eun-Ji (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University) ;
  • Lee, Dae-Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Department of Oriental Medicine Biotechnology, Kyung Hee University)
  • Received : 2015.04.28
  • Accepted : 2015.06.02
  • Published : 2015.09.30
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Abstract

The flowers of Chionanthus retusus Lindl. & Paxton were extracted with 80% aqueous MeOH at room temperature. The concentrated extract was partitioned as EtOAc, n-BuOH, and $H_2O$ fractions. From the EtOAc fraction, two triterpenoids and two sterols were isolated using the repeated silica gel ($SiO_2$) and octadecyl $SiO_2$ (ODS) column chromatographies. According to the results of physico-chemical and spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared. The chemical structures of the compounds were respectively determined as ursolic acid (1), corosolic acid (2), ${\beta}$-sitosterol (3), and daucosterol (4). All the compounds were isolated for the first time from the flowers of Chionanthus retusus Lindl. & Paxton.

이팝나무(Chionanthus retusus Lindl. & Paxton) 꽃은 실온에서 80% MeOH 수용액으로 추출하고 이 추출물을 EtOAC 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었다. EtOAc 분획에 대하여 silica gel 및 ODS column chromatography를 반복 실시하여 4종의 화합물을 분리 및 정제하였다. nuclear magnetic resonance, infrared, 및 Electronic ionization mass spectrometer 등을 해석하여, ursolic acid (1), corosolic acid (2), ${\beta}$-sitosterol (3), 그리고 daucosterol (4)로 구조동정 하였다. 분리한 4종의 화합물은 이팝나무 꽃으로부터 이번 실험에서 처음 분리 되었다.

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References

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