초록
황목련 꽃으로부터 3개의 alkyl glycoside 화합물을 분리, 동정하였다. 황목련 꽃을 80% MeOH에 추출하여 이를 EtOAc, n-BuOH, 그리고 $H_2O$ 층으로 계통분획을 실시하였다. 이중 EtOAc 분획에 대하여 silica gel과 ODS column chromatography를 반복 실시하여 3종의 화합물을 분리, 정제하였다. Nuclear magnetic resonance, infrared spectroscopy 및 mass spectrometry의 spectroscopic data를 해석하여 이 화합물들을 2-methylbutan-1-ol-${\beta}$-$\small{D}$-galactopyranoside (1), 2-methylbutan-1-ol-${\beta}$-$\small{D}$-glucopyranoside (2), 2-methylpropan-1-ol-${\beta}$-$\small{D}$-glucopyranoside (3)로 각각 동정하였다. 동정된 화합물 모두 황목련 꽃에서는 이번 연구에서 처음으로 분리, 동정되었다.
The flowers of Magnolia obovata were extracted with aqueous MeOH and fractionated into EtOAc, n-BuOH, and $H_2O$ fractination. Three alkyl glycosides were isolated from the EtOAc fraction through repeated silica gel and ODS column chromatography. The structures were identified to be 2-methylbutan-1-ol-${\beta}$-$\small{D}$-galacto-pyranoside (1), 2-methylbutan-1-ol-${\beta}$-$\small{D}$-glucopyranoside (2), and 2-methylpropan-1-ol-${\beta}$-$\small{D}$-glucopyranoside (3) on the basis of spectroscopic analyses such as fast atom bombardment mass spectrometry, infrared spectroscopy, 1D nuclear magnetic resonance (NMR) ($^1H$ and $^{13}C-NMR$), and 2D NMR (gCOSY, gHSQC, and gHMBC). These compounds were isolated for the first time from the flower of M. obovata in this study.