한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제30권3호
  • /
  • Pages.242-248
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  • 2015
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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보육시설 유아 사용 칫솔의 식중독 미생물 분포 및 독소 유전자

Prevalence and Toxin Genes of Food-Borne Pathogens Isolated from Toothbrush in Child Care Center

  • 김종승 (계명대학교 공중보건학과) ;
  • 김중범 (순천대학교 식품공학과)
  • Kim, Jong-Seung (Department of Public Health, KeiMyung University) ;
  • Kim, Jung-Beom (Department of Food Science and Technology, SunChon National University)
  • 투고 : 2015.07.02
  • 심사 : 2015.08.18
  • 발행 : 2015.09.30
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초록

어린이집 유아들이 사용하는 칫솔 및 양치 컵의 미생물 오염도와 분리된 식중독 미생물의 독소 유전자 및 독소 생산능력을 분석하여 칫솔과 양치 컵에 의해 발생할 수 있는 식중독을 예방하고자 칫솔 75개, 칫솔걸이 29개, 양치 컵 65개를 실험하였다. 일반세균수는 평균 5.3 log CFU 검출되었고 칫솔은 평균 6.7 log CFU 검출되어 가장 높은 오염도를 나타내었다. 대장균군은 칫솔 75건 중 41건 (54.7%), 칫솔걸이 29건 중 13건 (44.8%), 양치 컵 65건 중 29건 (44.6%)에서 검출되었으며 진균수는 평균 3.2 log CFU 검출되었고 칫솔이 평균 4.6 log CFU 검출되어 칫솔의 대장균군, 진균 오염도가 가장 높게 나타났다. Salmonella spp.는 모든 시료에서 검출되지 않았으나 B. cereus는 칫솔 75건 중 1건 (1.3%), 양치 컵 65건 중 2건 (3.1%)에서 검출되었고 S. aureus는 양치 컵 65건 중 1건 (1.5%)에서 검출되었다. 칫솔에서 분리된 B. cereus의 경우 nheA, nheB, nheC, hblC, hblD, hblA, entFM 등 7종의 설사독소 유전자가 검출되었고 양치 컵에서 분리된 B. cereus 2균주는 nheA, nheB, nheC, entFM 설사독소 유전자만 검출되었다. 칫솔에서 분리된 B. cereus의 경우 HBL, NHE 설사독소를 생산하였고 양치 컵에서 분리된 B. cereus 2균주는 모두 NHE 설사독소만을 생산하였다. B. cereus는 ${\beta}-lactam$계 항생제에 내성을 나타내었고 S. aureus는 ampicillin과 penicillin에만 내성을 나타내었다. 이러한 결과는 대부분의 칫솔과 양치 컵이 양치 후 젖은 상태로 화장실에 보관되거나 젖은 상태로 자외선 살균고에서 살균되는 등 부적절한 보관가 살균방법이 주요 원인으로 분석되었다. 따라서 면역력이 취약한 어린이집 유아가 사용하는 칫솔 및 양치컵을 건조한 후 자외선 살균고 등을 이용 살균하여 상대 습도가 낮은 건조한 곳에 보관하여야 할 것으로 판단되었다.

This study was performed to investigate the microbiological contamination on toothbrushes, toothbrush caps, and tooth cleaning cups in the child care centers and to evaluate the toxin genes, toxin production ability and antibiotic resistance of food-borne pathogens. The average number of total aerobic bacteria and fungi were 5.3 log CFU and 3.2 log CFU. Coliform bacteria were detected in 41 (54.7%) of 75 toothbrushes, 13 (44.8%) of 29 toothbrush caps, and 29 (44.6%) of 65 tooth cleaning cups. Salmonella spp. was not detected in all of samples but Bacillus cereus was isolated from 1 (1.3%) of 75 toothbrushes and 2 (3.1%) of 65 tooth cleaning cups. Staphylococcus aureus was detected in 1 (1.5%) of 65 tooth cleaning cups. The nheA, nheB, nheC, hblC, hblD, hblA and entFM toxin genes were possessed in B. cereus isolated from toothbrush which also produce NHE and HBL enterotoxins. S. aureus was resistant to ampicillin and penicillin, while B. cereus was resistant to ${\beta}-lactam$ antibiotics. These results indicated that the sanitary conditions of toothbrushes and tooth cleaning cups in the child care centers should be improved promptly. The UV sterilization after drying and then storage in dried condition is required to improve the sanitary condition of toothbrushes and tooth cleaning cups in the child care center.

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피인용 문헌

  1. A comparative analysis of micro-organism colonization of tooth-brushes in a public bath based on the storage technique vol.41, pp.4, 2017, https://doi.org/10.11149/jkaoh.2017.41.4.250