Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Combined Expression of Metastasis Related Markers Naa10p, SNCG and PRL-3 and its Prognostic Value in Breast Cancer Patients

  • Min, Li (Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)) ;
  • Ma, Ruo-Lan (Department of Thoracic Surgery, Peking University Cancer Hospital and Institute) ;
  • Yuan, Hua (Breast Center, Peking University Cancer Hospital and Institute) ;
  • Liu, Cai-Yun (Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)) ;
  • Dong, Bing (Department of Pathology, Peking University Cancer Hospital and Institute) ;
  • Zhang, Cheng (Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)) ;
  • Zeng, Yan (Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)) ;
  • Wang, Li (Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)) ;
  • Guo, Jian-Ping (Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)) ;
  • Qu, Li-Ke (Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education)) ;
  • Shou, Cheng-Chao (Department of Biochemistry and Molecular Biology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education))
  • Published : 2015.04.14
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Abstract

Combinations of multiple biomarkers representing distinct aspects of metastasis may have better prognostic value for breast cancer patients, especially those in late stages. In this study, we evaluated the protein levels of N-${\alpha}$-acetyltransferase 10 protein (Naa10p), synuclein-${\gamma}$ (SNCG), and phosphatase of regenerating liver-3 (PRL-3) in 365 patients with breast cancer by immunohistochemistry. Distinct prognostic subgroups of breast cancer were identified by combination of the three biomarkers. The Naa10p+SNCG-PRL-3-subgroup showed best prognosis with a median distant metastasis-free survival (DMFS) of 140 months, while the Naa10p-SNCG+PRL-3+subgroup had the worst prognosis with a median DMFS of 60.5 months. Multivariate analysis indicated Naa10p, SNCG, PRL-3, and the TNM classification were all independent prognostic factors for both DMFS and overall survival (OS). The three biomarker combination of Naa10p, SNCG and PRL-3 performed better in patients with lymph node metastasis, especially those with more advanced tumors than other subgroups. In conclusion, the combined expression profile of Naa10p, SNCG and PRL-3, alone or in combination with the TNM classification system, may provide a precise estimate of prognosis of breast cancer patients.

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References

  1. Al-Aidaroos A and Zeng Q (2010). PRL-3 phosphatase and cancer metastasis. J Cell Biochem, 111, 1087-98. https://doi.org/10.1002/jcb.22913
  2. Amsterdam A, Shezen E, Raanan C, et al (2012). Differential staining of gamma synuclein in poorly differentiated compared to highly differentiated colon cancer cells. Oncol Rep, 27, 1451-4.
  3. Arnesen T, Kong X, Evjenth R, et al (2005). Interaction between HIF-$1{\alpha}$ (ODD) and hARD1 does not induce acetylation and destabilization of HIF-$1{\alpha}$. FEBS Letters, 579, 6428-32. https://doi.org/10.1016/j.febslet.2005.10.036
  4. Bauer J, Shin D, Chun Y, et al (2009). Arrest defective-1 controls tumor cell behavior by acetylating myosin light chain kinase. PLoS ONE, 4, 7451. https://doi.org/10.1371/journal.pone.0007451
  5. Bilton R (2005). Arrest-defective-1 protein, an acetyltransferase, does not alter stability of hypoxia-inducible factor (HIF)-$1{\alpha}$ and is not induced by hypoxia or HIF. J Biol Chem, 280, 31132-40. https://doi.org/10.1074/jbc.M504482200
  6. Cox C, Shamehdi C and Glancy J (2011). Sentinel node micrometastasis in breast cancer: clinically significant or not? Breast J, 17, 335-6. https://doi.org/10.1111/j.1524-4741.2011.01114.x
  7. Czekierdowski A, Czekierdowska S, Wielgos M, et al (2006). The role of CpG islands hypomethylation and abnormal expression of neuronal protein synuclein-gamma (SNCG) in ovarian cancer. Neuro Endocrinol Lett, 27, 381-6.
  8. Franceschini A, Szklarczyk D, Frankild S, et al (2012). STRING v9.1: protein-protein interaction networks, with increased coverage and integration. Nucleic Acids Res, 41, 808-15.
  9. Gromyko D, Arnesen T, Ryningen A, et al (2010). Depletion of the human $N{\alpha}$-terminal acetyltransferase A induces p53-dependent apoptosis and p53-independent growth inhibition. Int J Cancer, 127, 2777-89. https://doi.org/10.1002/ijc.25275
  10. Guo J, Shou C, Meng L, et al (2007). Neuronal protein synuclein gamma predicts poor clinical outcome in breast cancer. Int J Cancer, 121, 1296-305. https://doi.org/10.1002/ijc.22763
  11. Guzinska-Ustymowicz K, Kisluk J, Terlikowski S, et al (2013). Expression of phosphatase of regenerating liver-3 (PRL-3) in endometrioid cancer and lymph nodes metastases. Adv Med Sci, 1, 7-12.
  12. Guzinska-Ustymowicz K, Pryczynicz A, Kemona A, Ustymowicz M (2011). Immunohistochemical assessment of PRL-3 (PTP4A3) expression in tumor buds, invasion front, central region of tumor and metastases of colorectal cancer. Adv Med Sci, 56, 39-43. https://doi.org/10.2478/v10039-011-0015-1
  13. Hibi T, Mori T, Fukuma M, et al (2009). Synuclein-gamma is closely involved in perineural invasion and distant metastasis in mouse models and is a novel prognostic factor in pancreatic cancer. Clin Cancer Res, 15, 2864-71. https://doi.org/10.1158/1078-0432.CCR-08-2946
  14. Holmes M, Chen W, Li L, et al (2010). Aspirin intake and survival after breast cancer. J Clin Oncol, 28, 1467-72. https://doi.org/10.1200/JCO.2009.22.7918
  15. Hua K, Tan C, Johansson G, et al (2011). N-${\alpha}$-acetyltransferase 10 protein suppresses cancer cell metastasis by binding PIX proteins and inhibiting Cdc42/Rac1 activity. Cancer Cell, 19, 218-31. https://doi.org/10.1016/j.ccr.2010.11.010
  16. Inaba S, Li C, Shi Y, et al (2005). Synuclein inhibits the mitotic checkpoint function and promotes chromosomal instability of breast cancer cells. Breast Cancer Res Treat, 94, 25-35. https://doi.org/10.1007/s10549-005-6938-0
  17. Jafferbhoy S, McWilliams B (2012). Clinical significance and management of sentinel node micrometastasis in invasive breast cancer. Clin Breast Cancer, 12, 308-12. https://doi.org/10.1016/j.clbc.2012.07.012
  18. Jemal A, Bray F, Center M, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90. https://doi.org/10.3322/caac.20107
  19. Jeong J, Bae M, Ahn M, et al (2002). Regulation and destabilization of HIF-1 alpha by ARD1. Cell, 111, 709-20. https://doi.org/10.1016/S0092-8674(02)01085-1
  20. Jia T, Liu Y, Liu J, et al (1999). Stimulation of breast cancer invasion and metastasis by synuclein gamma. Cancer Res, 59, 742-7.
  21. Kuo H, Lee D, Chen C, et al (2010). ARD1 Stabilization of TSC2 suppresses tumorigenesis through the mTOR signaling pathway. Sci Signal, 3, 9.
  22. Lee C, Ou D, Lee S, et al (2010). hNaa10p contributes to tumorigenesis by facilitating DNMT1-mediated tumor suppressor gene silencing. J Clin Invest, 120, 2920-30. https://doi.org/10.1172/JCI42275
  23. Lim J, Park J, Chun Y (2006). Human arrest defective 1 acetylates and activates ${\beta}$-catenin, promoting lung cancer cell proliferation. Cancer Res, 66, 10677-82. https://doi.org/10.1158/0008-5472.CAN-06-3171
  24. Liu C, Dong B, Lu A, et al (2010). Synuclein gamma predicts poor clinical outcome in colon cancer with normal levels of carcinoembryonic antigen. BMC Cancer, 10, 359. https://doi.org/10.1186/1471-2407-10-359
  25. Min L, Xu H, Wang J, et al (2013). N-${\alpha}$-acetyltransferase 10 protein is a negative regulator of 28S proteasome through interaction with $PA28{\beta}$. FEBS Letters, 587, 1630-7. https://doi.org/10.1016/j.febslet.2013.04.016
  26. Ming J, Liu N, Gu Y, et al (2009). PRL-3 facilitates angiogenesis and metastasis by increasing ERK phosphorylation and up-regulating the levels and activities of Rho-A/C in lung cancer. Pathol, 41, 118-26. https://doi.org/10.1080/00313020802579268
  27. Murrayrust T, Oldham N, Hewitson K, et al (2006). Purified recombinant hARD1 does not catalyse acetylation of Lys532 of HIF-$1{\alpha}$ fragments in vitro. FEBS Letters, 580, 1911-8. https://doi.org/10.1016/j.febslet.2006.02.012
  28. Ohkawa N, Sugisaki S, Tokunaga E, et al (2008). N-acetyltransferase ARD1-NAT1 regulates neuronal dendritic development. Genes Cells, 13, 1171-83.
  29. Ohkawa N, Sugisaki S, Tokunaga E, et al (2007). ARD1-NAT1 complex regulates neuronal dendritic arborization through ${\alpha}$-tubulin acetylation. Neurosci Res, 58, 88.
  30. Pan Z, Bruening W and Godwin A (2006). Involvement of RHO GTPases and ERK in synuclein-gamma enhanced cancer cell motility. Int J Oncol, 29, 1201-5.
  31. Peng L, Xing X, Li W, et al (2009). PRL-3 promotes the motility, invasion, and metastasis of LoVo colon cancer cells through PRL-3-integrin beta1-ERK1/2 and-MMP2 signaling. Mol Cancer, 8, 110.
  32. Polato F, Codegoni A, Fruscio R, et al (2005). PRL-3 phosphatase is implicated in ovarian cancer growth. Clin Cancer Res, 11, 6835-9. https://doi.org/10.1158/1078-0432.CCR-04-2357
  33. Polymeropoulos M, Lavedan C, Leroy E, et al (1997). Mutation in the gamma-synuclein gene identified in families with parkinson's disease. Science, 276, 20457.
  34. Rahimzadeh M, Baghestani A, Gohari M, Pourhoseingholi M (2014). Estimation of the cure rate in Iranian breast cancer patients. Asian Pac J Cancer Prev, 15, 4839-42. https://doi.org/10.7314/APJCP.2014.15.12.4839
  35. Saini K, Taylor C, Ramirez A, et al (2011). Role of the multidisciplinary team in breast cancer management: results from a large international survey involving 39 countries. Ann Oncol, 23, 853-9.
  36. Seo J, Cha J, Park J, et al (2010). Arrest defective 1 autoacetylation is a critical step in its ability to stimulate cancer cell proliferation. Cancer Res, 70, 4422-32. https://doi.org/10.1158/0008-5472.CAN-09-3258
  37. Siegel R, Naishadham D and Jemal A (2012). Cancer statistics, 2012. CA Cancer J Clin, 62, 10-29. https://doi.org/10.3322/caac.20138
  38. Singh V, Zhou Y, Marsh J, et al (2007). Synuclein-gamma targeting peptide inhibitor that enhances sensitivity of breast cancer cells to antimicrotubule drugs. Cancer Res, 67, 626-33. https://doi.org/10.1158/0008-5472.CAN-06-1820
  39. Surgucheva I, Park B, Yue B, et al (2005). Interaction of myocilin with gamma-synuclein affects its secretion and aggregation. Cell Mol Neurobiol, 25, 1009-33. https://doi.org/10.1007/s10571-005-8471-4
  40. Vairaktaris E, Yapijakis C, Serefoglou Z, et al (2008). Gene expression polymorphisms of interleukins-1 beta, -4, -6, -8, -10, and tumor necrosis factors-alpha, -beta: regression analysis of their effect upon oral squamous cell carcinoma. J Cancer Res Clin Oncol, 134, 821-32. https://doi.org/10.1007/s00432-008-0360-z
  41. Wang L (2006). Overexpression of phosphatase of regenerating liver-3 in breast cancer: association with a poor clinical outcome. Ann Oncol, 17, 1517-22. https://doi.org/10.1093/annonc/mdl159
  42. Wang Z, He Y, Cai S, et al (2008). Expression and prognostic impact of PRL-3 in lymph node metastasis of gastric cancer: its molecular mechanism was investigated using artificial microRNA interference. Int J Cancer, 123, 1439-47. https://doi.org/10.1002/ijc.23643
  43. Wu K, Huang S, Zhu M, et al (2013). Expression of synuclein gamma indicates poor prognosis of triple-negative breast cancer. Med Oncol, 30, 612. https://doi.org/10.1007/s12032-013-0612-x
  44. Zeng Y, Liu C, Dong B, et al (2013). Inverse correlation between Naa10p and MMP-9 expression and the combined prognostic value in breast cancer patients. Med Oncol, 30, 562. https://doi.org/10.1007/s12032-013-0562-3
  45. Zheng P, Liu Y, Chen L, et al (2010). Stathmin, a new target of PRL-3 identified by proteomic methods, plays a key role in progression and metastasis of colorectal cancer. J Proteome Res, 9, 4897-905. https://doi.org/10.1021/pr100712t

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