Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Blood Flow Improvement Effect of Bokbunja (Rubus coreanus) Seed Oil in High-Fat Diet-Fed Mouse Model

고지방식이 섭취 마우스를 이용한 복분자종자유의 혈행 개선 효과

  • 전혜린 (울산대학교 의과대학 의생명과학과) ;
  • 곽성민 (울산대학교 의과대학 의생명과학과) ;
  • 오수진 ((주)제이크린베리너리) ;
  • 남현수 ((주)제이크린베리너리) ;
  • 한두원 ((재)전북생물산업진흥원) ;
  • 송윤석 ((재)전북생물산업진흥원) ;
  • 송진우 ((재)전북생물산업진흥원) ;
  • 최경철 (울산대학교 의과대학 의생명과학과)
  • Received : 2015.04.07
  • Accepted : 2015.04.23
  • Published : 2015.08.31
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Abstract

Bokbunja (Rubus coreanus) is a Korean fruit and wild black raspberry that has antioxidant, anticancer, and beauty effects due to its abundant polyphenols and anthocyanins. The purpose of this study was to investigate the blood flow improvement effect of Bokbunja seed oil (BSO) in a high-fat diet-fed mouse model. We examined improvement of blood flow and its related biomarkers in vivo. Mice were divided into four groups; Control, high fat diet control (negative control, HFD), salmon oil control (positive control, HFD+commercial n-3 fatty acid), and BSO experiment groups (HFD+2 g/2,000 kcal, HFD+4 g/2,000 kcal). After the mice were sacrificed, plasma triglyceride, cholesterol, and blood flow-related biomarkers (coagulation factor 7, 12, serotonin, TXB2, PT, and aPTT) were measured in mouse blood and organs. BSO reduced blood viscosity through improvement of blood lipids (cholesterol and plasma triglycerides) as well as levels of blood coagulation factors and blood platelet activity. BSO also delayed blood coagulation time. Thus, we confirmed that BSO inhibits excessive blood clotting of blood vessels and improves blood flow. Taken together, these results suggest that BSO decreases plasma triglycerides and cholesterol and improves blood flow by regulating biomarkers.

혈행 개선 기능성을 평가하기 위하여 8주 동안 일반식이와 고지방식이를 제공하는 그룹에 오메가-3 지방산과 복분자종자유를 일정량 제공하는 실험을 실시하였다. 실험군은 일반식이 그룹과 고지방식이 그룹으로 나누어 각 그룹 내에 음성대조군, 양성대조군(오메가-3 지방산), 복분자종자유(2 g, 4 g/2,000 kcal) 급이군으로 구성하였다. 양성대조군과 복분자종자유는 매일 경구 투여되었고 실험기간 종료 후 동물을 희생시켜 혈액과 장기를 얻어 혈중중성지방 및 콜레스테롤, 혈행 개선에 관련된 바이오마커(coagulation factor7, 12, serotonin과 TXB2)를 측정하였는데, 그 결과 복분자종자유는 혈중지질의 농도 개선을 통해 혈액의 점도를 감소시키고 혈액의 응고를 억제하여 혈행을 개선하는 기능성이 있는 것을 확인하게 되었다. 따라서 복분자종자유를 이용한 건강기능식품 개발은 혈관질환 환자들에게 혈행 개선을 통한 건강증진 효과를 보여줄 것으로 판단된다.

Keywords

References

  1. Statistics Korea. 2014. Official cause of death statistics. Statistics Korea, Daejeon, Korea. p 4-43.
  2. Lee SJ, Ha WH, Choi HJ, Cho SY, Choi JW. 2010. Antiobesity and antihyperlipidemia of red crab shells in Sprague Dawley rat. J Chitin Chitosan 15: 156-163.
  3. Lee KH, Kim Y, Park E, Cho S. 2008. Study on dietary habit and effect of onion powder supplementation on serum lipid levels in early diagnosed hyperlipidemic patients. J Korean Soc Food Sci Nutr 37: 561-570. https://doi.org/10.3746/jkfn.2008.37.5.561
  4. Shin HH. 2002. Korean guidelines of hyperlipidemia treatment for prevention of atherosclerosis. Korean J Lipidol 12:226-228.
  5. Lim HJ, Cho KH, Choue R. 2005. The effects of functional tea (Mori Folium, Lycii Fructus, Chrysanthemi Flos, Zizyphi Fructus, Sesamum Semen, Raphani Semen) supplement with medical nutrition therapy on the blood lipid levels and antioxidant status in subjects with hyperlipidemia. J Korean Soc Food Sci Nutr 34: 42-56. https://doi.org/10.3746/jkfn.2005.34.1.042
  6. Ryu KH, Han HY, Lee SY, Jeon SD, Im GJ, Lee BY, Kim K, Lim KM, Chung JH. 2009. Ginkgo biloba extract enhances antiplatelet and antithrombotic effects of cilostazol without prolongation of bleeding time. Thromb Res 124: 328-334. https://doi.org/10.1016/j.thromres.2009.02.010
  7. Cho HJ, Nam KS. 2007. Inhibitory effect of ginkgolide B on platelet aggregation in a cAMP- and cGMP-dependent manner by activated MMP-9. J Biochem Mol Biol 40: 678-683. https://doi.org/10.5483/BMBRep.2007.40.5.678
  8. Nam SM, Ha EH, Suh YJ, Park H, Chang MH, Seo JH, Kim BM. 2008. Effect of obesity and blood lipid profiles on hyperlipidemia in adults aged over 40 years. Korean J Obes 17: 20-28.
  9. Moon JH. 2006. Effect of aerobic and resistance exercise to hyperlipidemia. Health & Medicine 8: 137-143.
  10. Hwang GH, Huh YR. 1999. A study of hyperlipidemia in Koreans－Ⅱ. Specially related to drinking, smoking, exercise and environmental characteristics and it's risk factor for hyperlipidemia－. Korean J Food & Nutr 12: 290-299.
  11. Klafke JZ, Arnoldi da Silva M, Fortes Rossato M, Trevisan G, Banderó Walker CI, Martins Leal CA, Olschowsky Borges D, Chitolina Schetinger MR, Noal Moresco R, Medeiros Frescura Duarte MM, Soares Dos Santos AR, Nazário Viecili PR, Ferreira J. 2012. Antiplatelet, antithrombotic, and fibrinolytic activities of Campomanesia xanthocarpa. Evid Based Complement Alternat Med 2012: 954748.
  12. Davies MJ, Thomas AC. 1985. Plaque fissuring－the cause of acute myocardial infarction, sudden ischaemic death, and crescendo angina. Br Heart J 53: 363-373. https://doi.org/10.1136/hrt.53.4.363
  13. Lau AJ, Toh DF, Chua TK, Pang YK, Woo SO, Koh HL. 2009. Antiplatelet and anticoagulant effects of Panax notoginseng: Comparison of raw and steamed Panax notoginseng with Panax ginseng and Panax quinquefolium. J Ethnopharmacol 125: 380-386. https://doi.org/10.1016/j.jep.2009.07.038
  14. May AE, Seizer P, Gawaz M. 2008. Platelets: inflammatory firebugs of vascular walls. Arterioscler Thromb Vasc Biol 28: 5-10. https://doi.org/10.1161/ATVBAHA.107.158915
  15. Ahn SH, Choo IS, Kim DM, Lim GH, Kim JH, Kim HW. 2008. Warfarin induced skin necrosis. J Korean Neurol Assoc 26: 142-145.
  16. Bircher AJ, Harr T, Hohenstein L, Tsakiris DA. 2006. Hypersensitivity reactions to anticoagulant drugs: diagnosis and management options. Allergy 61: 1432-1440. https://doi.org/10.1111/j.1398-9995.2006.01227.x
  17. Wong KS, Mok V, Lam WW, Kay R, Tang A, Chan YL, Woo J. 2000. Aspirin associated intracerebral hemorrhage: Clinical and radiologic features. Neurology 54: 2298-2301. https://doi.org/10.1212/WNL.54.12.2298
  18. Kim YH, Kim KW, Ahn SH, Oh MS, Ma HI, Kim YJ, Lee BC. 2014. Myotonic potentials in the myopathy induced by HMG-CoA reductase inhibitor. J Korean Neurol Assoc 32:186-189.
  19. Kim JM, Shin M. 2011. Characteristic of Rubus coreanus Miq. fruits at different ripening stages. Korean J Food Sci Technol 43: 341-347. https://doi.org/10.9721/KJFST.2011.43.3.341
  20. Park PJ, Lo SC, Han SS. 2004. Control of disease, insect pest and weed cultivation area of Rubus coreanus Miquel. J Life Sic Nat Res 26: 56-67.
  21. Kim SJ, Kim JY, Baek SH. 2011. Effect of composts fermented with Korean medicinal herb wastes on physiological activity of Rubus coreanus Miquel (Bokbunja). Korean J Plant Res 24: 243-252. https://doi.org/10.7732/kjpr.2011.24.2.243
  22. Cha HS, Youn AR, Park PJ, Choi HR, Kim BS. 2007. Comparison of physiological activities of Rubus coreanus Miquel during maturation. J Korean Soc Food Sci Nutr 36:683-688. https://doi.org/10.3746/jkfn.2007.36.6.683
  23. Cho YJ, Chun SS, Kwon HJ, Kim JH, Yoon SJ, Lee KH. 2005. Comparison of physiological activities between hotwater and ethanol extracts of Bokbunja (Rubus coreanum F.). J Korean Soc Food Sci Nutr 34: 790-796. https://doi.org/10.3746/jkfn.2005.34.6.790
  24. Kim ID, Kwon RH, Heo YY, Jung HJ, Kang HY, Ha BJ. 2008. Supercritical extraction of Oriental herb: Anti-aging and anti-wrinkle effects. Korean J Biotechnol Bioeng 23:529-534.
  25. Kim DH, Park JH, Kim JH, Kim CH, You JH, Kwon MC, Lee HY. 2005. Enhancement of immune activities of Ephedrae Herba and Rubi Fructus at low temperature extraction. Korean J Medicinal Crop Sci 13: 81-86.
  26. Lee JW, Do JH. 2000. Determination of total phenolic compounds from the fruit of Rubus coreanum and antioxidative activity. J Korean Soc Food Sci Nutr 29: 943-947.
  27. Yoon I, Wee JH, Moon JH, Ahn TH, Park KH. 2003. Isolation and identification of quercetin with antioxidative activity from the fruits of Rubus coreanum Miquel. Korean J Food Sci Technol 35: 499-502.
  28. Kim JH, Kim CH, Kim HS, Kwon MC, Song YK, Seong NS, Lee SE, Yi JS, Kwon OW, Lee HY. 2006. Effect of aqueous extracts from Rubus coreanus Miquel and Angelica gigas Kakai on anti-tumor and anti-stress activities in mice. Korean J Medicinal Crop Sci 14: 206-211.
  29. Lee SJ, Lee MJ, Ko YJ, Choi HR, Jeong JT, Choi KM, Cha JD, Hwang SM, Jung HK, Park JH, Lee TB. 2013. Effect of extracts of unripe black raspberry and red ginseng on cholesterol synthesis. Korean J Food Sci Technol 45:628-635. https://doi.org/10.9721/KJFST.2013.45.5.628
  30. Lee MJ, Lee SJ, Choi HR, Lee JH, Jeong JT, Choi KM, Cha JD, Hwang SM, Park JH, Lee JH, Lee TB. 2014. Cholesterol improvement effects of co-treatment with black raspberry and red ginseng extracts in mice fed a high cholesterol diet. J Korean Soc Food Sci Nutr 43: 1491-1499. https://doi.org/10.3746/jkfn.2014.43.10.1491
  31. Choi Y, Lee YJ, Lee S, Chae C, Park S, Kim SS, An H, King D, Han C, Hong H. 2012. Effect of concentrated dangyooja-derived flavonoids extract added to citrus beverage on obesity and blood lipids in rats. Kor J Hort Sci Technol 30: 214-219.
  32. Lee SJ, Park HJ, Song Y, Jang SH, Goo AJ, Ko YG, Cho JH. 2014. Antioxidant activity and anti-obesity effect of Coprinus comatus in Zucker rat (fa/fa). Korean J Ver Serv 37: 51-58. https://doi.org/10.7853/kjvs.2014.37.1.51
  33. Lee CM. 2000. The effect of the administration of red ginseng and vitamin E on the activation of antioxidizing enzyme and the formation of lipid peroxidation in blood. The Korean Journal of Physical Education 39: 476-488.
  34. Lee JW, Do JH. 2000. Determination of total phenolic compounds from the fruit of Rubus coreanum and antioxidative activity. J Korean Soc Food Sci Nutr 29: 943-947.
  35. Chung SH, Moon YJ, Kim SG, Kim KY, Lee KT, Kim HK, Whang WK. 2008. Isolation of flavonoids from Carthami flos and their antioxidative activity. Yakhak Hoeji 52: 241-251.
  36. Nam HS. 2012. Rubus coreanus Miquel oil composition, use and preparation method thereof. Korean Patent 10-1168169-0000.
  37. Hong SG, Park CK, Lee MJ, Chung SG, Lee YH, Hyun SH. 2009. Acute oral toxicity and anti-obesity effect of diglyceride preparation containing conjugated linoleic acid in rat. Korean J Food Sci Technol 41: 320-325.
  38. Moon HS, Lee HG, Seo JH, Chung CS, Kim TG, Kim IY, Lim KW, Seo SJ, Choi YJ, Cho CS. 2006. Down-regulation of PPAR$\gamma$2-induced adipogenesis by PEGylated conjugated linoleic acid as the pro-drug: Attenuation of lipid accumulation and reduction of apoptosis. Arch Biochem Biophys 456: 19-29. https://doi.org/10.1016/j.abb.2006.10.002
  39. Davidson MH. 2006. Mechanisms for the hypotriglyceridemic effect of marine omega-3 fatty acids. Am J Cardiol 98: 27-33.
  40. Han KH. 2012. Omega-3-fatty acid and triglyceride. Korean J Med 83: 724-727. https://doi.org/10.3904/kjm.2012.83.6.724
  41. Jun HJ, Ko JH, Jung HS, Yoon CS, Kim TK, Kwon MJ, Lee SH, Suk JH, Kim MK, Kim DK, Park JH. 2011. Effect of omega-3 fatty acids on low density lipoprotein subfraction, adiponectin and apolipoprotein B in type 2 diabetic patients. Endocrinol Metab 26: 218-224. https://doi.org/10.3803/EnM.2011.26.3.218
  42. Suriawinata AA, Thung SN. 2006. Acute and chronic hepatitis. Semin Diagn Pathol 23: 132-148. https://doi.org/10.1053/j.semdp.2006.11.001
  43. Whitworth JR, Mack CL, O'Connor JA, Narkewicz MR, Mengshol S, Sokol RJ. 2006. Acute hepatitis and liver failure associated with influenza A infection in children. J Pediatr Gastroenterol Nutr 43: 536-538. https://doi.org/10.1097/01.mpg.0000232332.00677.3d
  44. Ebert EC. 2006. Hypoxic liver injury. Mayo Clin Proc 81:1232-1236. https://doi.org/10.4065/81.9.1232
  45. Tang W. 2007. Drug metabolite profiling and elucidation of drug-induced hepatotoxicity. Expert Opin Drug Metab Toxicol 3: 407-420. https://doi.org/10.1517/17425255.3.3.407
  46. Jeon NL, Kim BS, Kim YK, Hong SJ. 2000. Etiology and clinical features of severe acute viral lower respiratory tract infections in children. Korean J Pediatr 43: 1558-1568.
  47. Wang J, Atchison RW, Walpusk J, Jaffe R. 2001. Echovirus hepatic failure in infancy: Report of four cases with speculation on the pathogenesis. Pediatr Dev Pathol 4: 454-460. https://doi.org/10.1007/s10024001-0043-0
  48. Lyu CJ. 2011. Introduction to coagulation system. J Korean Soc Neonatol 18: 1-5. https://doi.org/10.5385/jksn.2011.18.1.1
  49. Vine AK. 2009. Recent advances in haemostasis and thrombosis. Retina 29: 1-7. https://doi.org/10.1097/IAE.0b013e31819091dc
  50. Duerschmied D, Suidan GL, Demers M, Herr N, Carbo C, Brill A, Cifuni SM, Mauler M, Cicko S, Bader M, Idzko M, Bode C, Wagner DD. 2013. Platelet serotonin promotes the recruitment of neutrophils to sites of acute inflammation in mice. Blood 121: 1008-1015. https://doi.org/10.1182/blood-2012-06-437392
  51. Hallback I, Hagg S, Eriksson AC, Whiss PA. 2012. In vitro effects of serotonin and noradrenaline reuptake inhibitors on human platelet adhesion and coagulation. Pharmacol Rep 64: 979-983. https://doi.org/10.1016/S1734-1140(12)70894-0
  52. Kidson-Gerber G, Weaver J, Gemmell R, Prasan AM, Chong BH. 2010. Serum thromboxane B2 compared to five other platelet function tests for the evaluation of aspirin effect in stable cardiovascular disease. Heart Lung Circ 19: 234-242. https://doi.org/10.1016/j.hlc.2009.11.002