Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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VvpM Induces Human Cell Death via Multifarious Modes Including Necroptosis and Autophagy

  • Lee, Mi-Ae (Department of Life Science, Sogang University) ;
  • Kim, Jeong-A (Department of Life Science, Sogang University) ;
  • Shin, Mee-Young (Department of Environmental Medical Biology, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine) ;
  • Lee, Jeong K. (Department of Life Science, Sogang University) ;
  • Park, Soon-Jung (Department of Environmental Medical Biology, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine) ;
  • Lee, Kyu-Ho (Department of Life Science, Sogang University)
  • Received : 2015.01.06
  • Accepted : 2015.01.14
  • Published : 2015.02.28
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Abstract

VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus, induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). The percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1, indicating that VvpM-mediated cell death occurs through necroptosis. The appearance of autophagic vesicles and lipidated form of light-chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, the multifarious action of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.

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