The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Physicochemical and Sensory Properties of Red Pepper Extract treated with Enzyme Complex

복합효소를 이용한 고추 추출액의 이화학적 및 관능적 특성

  • Lee, Jong-Yeol (Dept. of Food and Nutrition, Korea National University of Transportation) ;
  • Choi, Gu-Hee (Dept. of Food and Nutrition, Korea National University of Transportation) ;
  • Lee, Kyung-Haeng (Dept. of Food and Nutrition, Korea National University of Transportation)
  • 이종열 (한국교통대학교 식품영양학과) ;
  • 최구희 (한국교통대학교 식품영양학과) ;
  • 이경행 (한국교통대학교 식품영양학과)
  • Received : 2015.08.07
  • Accepted : 2015.08.13
  • Published : 2015.08.31
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Abstract

The ground red pepper puree was treated with cellulase (C treatment), pectinase (P treatment), amylase (A treatment) and/or enzyme complex (CP, CA, PA and CPA treatment) for 2~8 hours to improve the yield and bioactivity of extracts. And physicochemical and sensory properties of red pepper extracts were evaluated. The extraction yield of the control was 38.84%, which was lower than those of the enzyme treatments. And extraction yields of enzyme complex treatments were higher than that of single enzyme treatments. Especially, extraction yield was increased to 74.37% by cellulase + pectinase + amylase complex treatment (CPA treatment). The soluble solid and reducing sugar contents were higher in the extracts treated with enzymes compared with the control. CA and CPA treatment showed the highest soluble solid and reducing sugar contents. No significant changes in lightness, redness and yellowness of the control and the samples by enzyme treatments were observed during 2~8 hours experiments. The sensory evaluation results revealed that panelists preferred the extracts with enzyme treatments to the control. Therefore, enzyme treatment for red pepper extracts is a good method to improve the yield and sensory properties.

고추 추출액의 수율 및 생리적 기능성을 향상시키기 위하여 고추에 cellulase(C 처리군), pectinase(P 처리군), amylase(A 처리군)를 각각 또는 이들 효소들을 혼합(CP, CA, PA 및 CPA 처리군) 첨가하여 2~8시간동안 가수분해시킨 후 가열처리하고, 여과시킨 추출액에 대하여 이화학적 및 관능적 평가를 측정하였다. 효소 처리 고추 추출액의 수율은 효소 처리를 하지 않았을 때 38.84% 정도로 매우 낮게 나타났지만, 효소처리군이 높은 수율을 보였고, 효소 단독처리군보다는 병용처리 시 수율이 증가하였으며, 효소 처리 시간이 길어질수록 유의적으로 수율이 증가하는 것으로 나타났다. 특히 cellulase + pectinase + amylase(CPA) 복합처리군은 추출수율이 74.37%까지 증가하였다. 가용성 고형분의 함량변화는 대조군의 경우에는 8.51% 를 나타내었으나, 효소처리군은 대조군보다 높은 함량을 나타내었으며, CA 혼합처리군과 CPA 혼합처리군이 가장 높은 가용성 고형분 함량을 보였다. 환원당의 함량 또한 효소 처리에 의하여 증가하는 것으로 나타났다. 효소 처리에 의한 색도의 변화에서는 대조군과 효소처리군 간에 색도의 변화를 보이지는 않았다. 효소 처리한 고추 추출액에 대한 관능검사 결과에서는 전반적으로 효소 처리를 하지 않은 대조군에 비하여 효소 처리 시 기호도 면에서 우수한 것으로 나타나, 고추 추출물 제조를 위한 효소 처리는 수율 및 기호도 증진을 위한 좋은 방법으로 사료된다.

Keywords

References

  1. Chang UJ, Kim DG, Kim JM, Suh HJ, Oh SH. 2003. Weight reduction effect of extract of fermented red pepper on female college students. Korean Soc Food Sci Nutr 32:479-484 https://doi.org/10.3746/jkfn.2003.32.3.479
  2. Farrell KT. 1981. Spices, condiments and seasonings. AVI, New York, USA. p 3
  3. Hasler CM. 1998. Functional foods: Their role in disease prevention and health promotion. Food Technol(Chicago) 52: 63-70
  4. Henry CJK, Emery B. 1985. Effect of spiced food on metabolic rate. Hum Nutr Clin Nutr 40C:165-168
  5. Im GY, Ma JY, Kim KW, Choi JK, Kang DK, Kwon TR, Jang SY, Jeong YJ. 2011. Quality characteristics of 4 year-old ginseng by enzymatic hydrolysis conditions. J Korean Soc Food Sci Nutr 40:229-234 https://doi.org/10.3746/jkfn.2011.40.2.229
  6. Jang SJ, Jo YJ, Seo JH, Kim OM, Jeong YJ. 2014. Enzyme treatment for clarification of spoiled oriental melon juice. Korean J Food Preserv 21:506-511 https://doi.org/10.11002/kjfp.2014.21.4.506
  7. Jawada T, Watanabe T, Takaishi T, Tanaka T, Iwai K. 1986. Capsaicin induced ${\beta}$-adrenergic action on energy metabolism in rats: influence of capsaicin on oxygen consumption, the respiratory quotient and substrate utilization. Proc Soc Exp Biol Med 183:250-256 https://doi.org/10.3181/00379727-183-42414
  8. Jeon KU, Han JY, Choi YM, Lee SM, Kim HT, Lee JS. 2008. Antioxidant and antiproliferative activity of pepper (Capsicum annuum L.) leaves. J Korean Soc Food Sci Nutr 37:1079-1083 https://doi.org/10.3746/jkfn.2008.37.8.1079
  9. Kim BY, Lee CG, Whang WK, Huh JD. 1989. Studies on the extraction of active components in Ginkgo biloba leaves by enzyme treatments. Korean J Pharmacogn 20:43-47
  10. Kim JE, Joo SI, Seo JH, Lee SP. 2009. Antioxidant and ${\alpha}$-glucosidase inhibitory effect of tartary buckwheat extract obtained by the treatment of different solvents and enzymes. J Korean Soc Food Sci Nutr 38:989-995 https://doi.org/10.3746/jkfn.2009.38.8.989
  11. Kim JP, Kim EH, Kim SU, Kwon TK, Choi KS. 2010. Capsaicin sensitizes malignant glioma cells to TRAIL-mediated apoptosis via DR5 upregulation and survivin downregulation. Carcinogenesis 31:367-375 https://doi.org/10.1093/carcin/bgp298
  12. Kim MJ, Lim KR, Jung TK, Yoon KS. 2007. Anti-aging effects of Astragalus membranaceus root extract. J Soc Cosmet Korea 33:33-40
  13. Kim NM, Lee JS, Lee BH. 1999. Effects of ${\beta}$-amylase and trans glucosidase on the qualities of red ginseng extract. J Ginseng Res 23:93-98
  14. Kwon DJ, Kim YJ, Lee S, Yoo JY. 1998. Technical development of hot sauce with red pepper. Korean J Food Sci Rechnol 30:391-396
  15. Kwon SC, Choi GH, Hwang GH, Lee KH. 2010. Physicochemical property and antioxidative activity of hot-water extracts from enzyme hydrolysate of Astragalus membranaceus. J Korean Soc Food Sci Nutr 39:406-413 https://doi.org/10.3746/jkfn.2010.39.3.406
  16. Kwon SC, Jo C, Lee KH. 2009a. Gamma irradiation for sanitation of vegetable fresh juice containing non-thermal process materials. J Korean Soc Food Sci Nutr 38:964-969 https://doi.org/10.3746/jkfn.2009.38.7.964
  17. Lee S, Yoo KM, Park JB, Hwang IK. 2012a. Development of value-added hot sauce products with Korean chili peppers (Capsicum annuum L.). Korean J Food Cookery Sci 28:257-263 https://doi.org/10.9724/kfcs.2012.28.3.257
  18. Lee S, Yoo KM, Song SR, Park JB, Hwang IK. 2012b. Development of value-added ketchup products with Korean chile peppers (Capsicum annuum L.) and their sensory evaluation. Korean J Food & Nutr 25:9-16 https://doi.org/10.9799/ksfan.2012.25.1.009
  19. Manjeshwar SB, Ganesh CJ, Shaial KR, Kiran BS. 2003. Evaluation of nitric oxide scavenging activity of certain spices in vitro: A preliminary study. Nahrung 47:261-264 https://doi.org/10.1002/food.200390061
  20. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426-428 https://doi.org/10.1021/ac60147a030
  21. Park CS, Oh EH, Jeong HS, Yoon HS. 2009. Quality characteristics of the germinated brown rice wine added with red pepper. J Korean Soc Food Sci Nutr 38:1090-1096 https://doi.org/10.3746/jkfn.2009.38.8.1090
  22. Song W, Derito CM, Liu K, He S, Liu RH. 2010. Cellular antioxidant activity of common vegetables. J Agr Food Chem 58:6621-6629 https://doi.org/10.1021/jf9035832
  23. Song WY, Yang JA, Ku KH, Choi JH. 2009. Effect of red pepper seeds powder on antioxidative system and oxidative damage in rats fed high-fat.high-cholesterol diet. J Korean Soc Food Sci Nutr 38:1161-1166 https://doi.org/10.3746/jkfn.2009.38.9.1161
  24. Surh YJ, Han SS, Keum YS, Seo HJ, Lee SS. 2000. Inhibitory effects of curcumin and capsaicin on phorbol ester-induced activation of enkaryotic transcription factors, NF-Kappa and AP-1. Biofactors 107-112
  25. Youn SJ, Kim GE, Jeong YI. 2003. Extract characteristic of old pumpkin on enzyme treatment. Korean J Food Preserv 10: 302-307