The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Anti-inflammatory Activities of Methanolic Extracts from Different Rose Cultivars

품종별 장미꽃 메탄올 추출물의 항염증 효과

  • Lee, Seon-Mi (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Li, Lin (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Sung, Jeehye (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Yang, Jinwoo (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Kim, Younghwa (School of Food Biotechnology and Nutrition, Kyungsung University) ;
  • Jeong, Heon Sang (Dept. of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Junsoo (Dept. of Food Science and Biotechnology, Chungbuk National University)
  • 이선미 (충북대학교 식품생명공학과) ;
  • 이림 (충북대학교 식품생명공학과) ;
  • 성지혜 (충북대학교 식품생명공학과) ;
  • 양진우 (충북대학교 식품생명공학과) ;
  • 김영화 (경성대학교 식품응용공학부) ;
  • 정헌상 (충북대학교 식품생명공학과) ;
  • 이준수 (충북대학교 식품생명공학과)
  • Received : 2015.07.21
  • Accepted : 2015.08.03
  • Published : 2015.08.31
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Abstract

The genus Rosa (Rosaceae) is an abundant source of phenolics and is traditionally used as a food supplement and as herbal medicine. Various plant phenolics are known to have anticancer, antioxidant, and anti-inflammatory properties. In this study, we investigated the anti-inflammatory effects of rose methanolic extracts (RMEs) from four different rose cultivars (Macarena, Onnuri, Oklahoma, and Colorado) in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Our results demonstrated that pretreatment of REMs ($500{\mu}g/mL$) significantly reduced NO production by suppressing iNOS protein expression in LPS-stimulated cells. Anti-inflammatory effects by RMEs were observed in the following order: Oklahoma > Colorado > Onnuri > Macarena. Consistent with this finding, RMEs inhibited the translocation of $NF-{\kappa}B$ from the cytosol to the nucleus via the suppression of $I{\kappa}B{\alpha}$ phosphorylation and also inhibited LPS-stimulated $NF-{\kappa}B$ transcriptional activity. These findings suggest that RMEs exert anti-inflammatory actions and help to elucidate the mechanisms underlying the potential therapeutic values of RMEs. Therefore, RMEs could be regarded as a potential source of natural anti-inflammatory agents.

본 연구에서는 장미꽃 methanol 추출물의 항염증 활성을 조사하기 위하여 LPS에 의해 염증이 유도된 RAW 264.7 대식세포에서 염증억제 효과를 알아보았다. 염증 억제의 지표로서는 세포가 방출하는 NO 생성량과 iNOS 및 $NF-{\kappa}B$ 발현 정도를 측정하였다. 실험 결과, RAW 264.7 대식세포에 대한 품종별 장미꽃 methanol 추출물($500{\mu}g/mL$)이 NO의 함량을 감소시키는 경향을 나타내었다. NO의 생성에 영향을 미치는 iNOS 단백질의 발현량을 측정한 결과, LPS 처리에 의해 활성화된 iNOS 단백질의 발현이 장미꽃 methanol 추출물 처리 시 유의적으로 수준으로 억제하는 경향을 보였다. Luciferase activity를 실행한 결과, LPS로 자극한 세포와 비교하였을 때 염증과 관련된 $NF-{\kappa}B$ promoter activity가 장미꽃 methanol 추출물 처리시 현저히 감소하는 경향을 나타내었고, 세포질의 $I{\kappa}B{\alpha}$의 인산화를 저해함으로써 전사요소인 $NF-{\kappa}B$ p65, p50을 핵 속으로 유리시키는 과정을 억제하였다. 이 결과로 장미꽃 methanol 추출물이 전사단계에서 저해활성을 나타낸다는 것을 확인하였다. 본 연구결과, 장미꽃 methanol 추출물은 항염증 효과를 나타냄에 따라 만성 질환 예방을 위한 기능성 식품의 원료로 활용될 수 있을 것으로 여겨진다.

Keywords

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