Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Pretreatment of Sugarcane Molasses and Citric Acid Production by Candida zeylanoides

사탕수수당밀의 전처리법과 Candida zeylanoides에 의한 시트르산의 생산

  • Kim, Kee Hyuk (Department of Culinary Arts, WooSong University) ;
  • Lee, Ho-Young (Department of Biochemistry, University of Washington) ;
  • Lee, Chan Yong (Department of Microbiology and Biotechnology, DaeJeon University)
  • 김기혁 (우송대학교 외식조리학부) ;
  • 이호영 (워싱턴주립대학교 생화학과) ;
  • 이찬용 (대전대학교 생명과학부)
  • Received : 2015.03.26
  • Accepted : 2015.05.22
  • Published : 2015.06.28
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Abstract

Citric acid is produced via submerged fermentation using yeasts. Among eight different strains of yeast, Candida zeylanoides was chosen as the strain for producing citric acid and optimized for various C/N ratios and effects of phosphate or Fe2+ ions in a clean carbon source medium (glucose: fructose, 1:1). The yield of citric acid was maximized at a C/N ratio of 40/1, a phosphate addition of 1.0 g/l, and an Fe2+ ion concentration of less than 50 mg/l, yielding up to 91 g/L in the broth with 18.5 g/l of isocitric acid in a six-day fermentation period using a pre-treated molasses medium. The yield of batch culture was 0.51 (Yp/s, g/g) in a 5 L-Jar fermenter.

효모의 액침배양법에 의한 구연산을 발효생산하였다. 여덟 가지 구연산 발효균주의 생산성을 비교하여 가장 생산성이 높은 Candida zeylanoides를 선발하였다. 발효조건의 최적화를 위하여 glucose와 fructose 혼합당 배지에서 C/N 비, 인산염의 농도, Fe2+ 이온의 최적 농도를 결정하였다. 삼각 플라스크 실험결과 C/N 비는 40/1이 가장 좋았으며, 최적화된 인삼염과 Fe2+ 농도에서 구연산 91.4 g/l와 이소-구연산 19.8 g/l를 생성하였다. 이 결과를 토대로 TCPH 전처리 당밀 배지를 사용한 실험에서는 인삼염 농도 1.0 g/l, Fe2+ 이온 50 mg/l 이하의 농도에서 구연산 91 g/l와 이소-구연산 18.5 g/l를 생성하였다. 5 L-Jar 발효조를 사용한 실험에서의 구연산 수율(Yp/s, g/g)은 0.51이었다.

Keywords

References

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