한국유기농업학회지 (Korean Journal of Organic Agriculture)

  • 제23권2호
  • /
  • Pages.323-334
  • /
  • 2015
  • /
  • 1229-3571(pISSN)
  • /
  • 2287-819X(eISSN)
한국유기농업학회 (Korean Association of Organic Agriculture)
권호 표지
DOI QR코드

DOI QR Code

오배자(Schlechtendalia chinensis)로부터 수박 과실썩음병 병원균(Acidovorax avenae subsp. citrulli)에 대한 항균 활성물질 탐색

Isolation of Antimicrobial Active Substances from Chinese Gall Nut (Schlechtendalia chinensis) against Watermelon Fruit Rot Pathogens (Acidovorax avenae subsp. Citrulli)

  • 김현우 (경북대학교 대학원 생태환경시스템학과) ;
  • 최용화 (경북대학교 생태환경대학 생태환경시스템학부)
  • 투고 : 2015.04.07
  • 심사 : 2015.06.11
  • 발행 : 2015.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 수박 과실썩음병의 원인균인 A. avenae subsp. citrulli에 대해 항균활성을 갖는 친환경 유기농자재를 개발할 목적으로 오배자(S. chinensis)를 대상으로 수행되었다. 오배자를 MeOH로 추출하여 용매분획을 하였고, 용매분획 중에서 가장 강한 활성을 나타낸 hexane fraction을 column chromatography로 분리하여 활성이 강한 분획들을 GC-MS로 분석하였다. GC chromatogram 상의 주요 peak에 해당하는 mass spectrum과 Wiley library를 비교하여 profiling한 결과, 지방산인 myristic acid, palmitic acid와 3-n-pentadecylphenol이 주요 물질로 검출되었다. 이들 검출 화합물의 항균활성을 검정하기 위하여 표준품을 사용하여 bioassay한 결과, myristic acid와 3-n-pentadecylphenol 화합물이 강한 활성을 보였다. 따라서 오배자로부터 분리한 myristic acid와 3-n-pentadecylphenol 화합물이 항균 활성물질인 것을 구명하였다.

This study was conducted to develop environment-friendly agricultural products with anti-microbial activity against Acidovorax avenae subsp. citrulli as a pathogen of bacterial fruit blotch in cucurbit. Schlechtendalia chinensis was extracted by MeOH and solvent fraction. The hexane fraction, which showed highest value of anti-microbial activity, was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to MS database of Wiley library. As a result, myristic acid, palmitic acid and 3-n-pentadecylphenol were identified as maine compounds showing antimicrobial activity against A. avenae subsp. citrulli. Bioassay using commercial myristic acid, palmitic acid and 3-n-pentadecylphenol to test for the anti-microbial activity conformed the anti-microbial activity of potential active compounds, and myristic acid and 3-n-pentadecylphenol showed strong activity. In conclusion, myristic acid and 3-n-pentadecylphenol identified from S. chinensis were anti-microbial chemicals.

키워드

참고문헌

  1. Assouline, I., H. Milshtein, M. Mizrahi, E. Levy, and I. S. Ben-Zeev. 1997. Acidovorax avenae subsp. citrulli Transmitted by Solanaceous seed. Phytoparasitica. 25: 2-3.
  2. Cha, J. Y., S. E. Ha, S. M. Sim, J. K. Park, Y. O. Chung, H. J. Kim, and N. B. Park. 2008. Antimicrobial effects of ethanol extracts of Korea endemic herb plants. J Life Sci. 18: 228-233. https://doi.org/10.5352/JLS.2008.18.2.228
  3. Choi, I., H. S. Chang, Y. M. Yun, and J. C. Um. 2002. Antimicrobial activity of medicinal herbs against Staphylococcus aureus and Salmonella gallinarum. Kor J Microbiol Biotechnol. 30: 177-183.
  4. Hopkins, D. L., J. D. Cucuzza, and J. C. Watterson 1996 Wet seed treatments for the control of bacterial fruit blotch of watermelon. Plant Dis. 30: 529-532.
  5. Hopkins, D. L. and N. C. Schenck 1971 Bacterial leaf spot of watermelon caused by Pseudomonas lachrymans. Phytopathol. 62: 542-545.
  6. Hopkins, D. L. and C. M. Thomson 2002 Seed transmission of Acidovorax avenae subsp. citrulli in cucurbits. HortSci. 37: 924-926.
  7. Isakeit, T., M. C. Black, L. W. Barnes, and J. B. Jones 1997 First report of infection of honeydew with Acidovorax avenae subsp. citrulli. Plant Dis. 81: 694.
  8. Jo, H. C., K. S. Han, and E. Y. An. 2000. Gall formation on different age, habitat, and parasite position in Rhus javanica L. Kor J Med Crop Sci. 8: 304-311.
  9. Langston, D. B. Jr., R. D. Walcott, R. D. Gitaitis, and F. H. Sanders Jr. 1999 First reportof a fruitrot pumpkin caused by Acidovorax avenae pv. citrulli in Georgia. Plant Dis. 83: 199.
  10. Latin, R. X. and D. L. Hopkins 1995 Bacterial fruit blotch of watermelon: the hypothetical exam question becomes reality. Plant Dis. 79: 761-765. https://doi.org/10.1094/PD-79-0761
  11. Latin, R. X. and K. K. Rane 1990 Bacterial fruit blotch of watermelon in Indiana. Plant Dis. 74: 331.
  12. Lessl, J. T., A. Fessehaie, and R. R. Walcott 2007 Colonization of female watermelon blossoms by Acidovorax avenae subsp. Citrulli and the relationship between blossom inoculum dosage and seed infestation. J. Phytopathol. 155: 114-121. https://doi.org/10.1111/j.1439-0434.2007.01204.x
  13. Martin, H. L., R. G. O'Brien, and D. V. Abbott 1999 First report of Acidvorax avenae subsp. cirulli as a pathogen of cucumber. Plant Dis. 83: 965.
  14. Rane, K. K. and R. X. Latin 1992 Bacterial fruit blotch of watermelon-association of the pathogen with seed. Plant Dis. 76: 509-512. https://doi.org/10.1094/PD-76-0509
  15. Seo, S. T., J. H. Park, J. S. Lee, K. S. Han, and S. R. Jung 2006 Bacterial fruit blotch of melon caused by Acidovorax avenae subsp. citrulli. Res. Plant Dis. 12: 185-188. https://doi.org/10.5423/RPD.2006.12.3.185
  16. Somodi, G. C., J. B. Jones, D. L. Hopkins, R. E. Stall, T. A. Kucharek, N. C. Hodge, and J. C. Watterson. 1991. Occurrence of a bacterial watermelon fruit blotch in Florida. Plant Dis. 75: 1053-1056. https://doi.org/10.1094/PD-75-1053
  17. Song, W. Y., H. M. Kim, I. Y. So, and Y. K. Kang. 1991. Pseudomonas pseudoalcaligenes subsp. citrulli: The causal agent of bacterial fruit blotch rot on watermelon. Kor. J. Plant Pathol. 7: 177-182.
  18. Song, W, Y., Kim, H, M., Kang, M, H. 2000. A new selsctive medium for detecting Acid Acidovorax avenae subsp. avenae in rice seeds. Plarhol. J. 16: 236-241.
  19. Sowel, G. Jr. and N. Schaad. 1979. Pseudomonas pseudoalcaligenes subsp. citrulli on watermelon: Seed transmission and resistance of plant introductions. Plant Dis. Rep. 63: 437-441.
  20. Wall, G. C., V. M. Santos, F. J. Cruz, D. A. Nelson, and I. Cabrera. 1990. Outbreak of watermelon fruit blotch in the Mariana islands. Plant Dis. 74: 80.