Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice

  • Lee, Young Seok (Department of Pediatric Cardiology, Dong-A University Hospital) ;
  • Park, Min Jung (Research Center for Anti-Aging Technology Development, Pusan National University) ;
  • Park, Sea Hee (Department of Obstetrics and Gynecology, Good Moonhwa Hospital) ;
  • Koo, Ja Seong (Department of Obstetrics and Gynecology, Good Moonhwa Hospital) ;
  • Moon, Hwa Sook (Department of Obstetrics and Gynecology, Good Moonhwa Hospital) ;
  • Joo, Bo Sun (Research Center for Anti-Aging Technology Development, Pusan National University)
  • Received : 2015.04.28
  • Accepted : 2015.06.01
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age. Methods: Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of $15-20{\mu}m$. Results: In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones. Conclusion: These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls.

Keywords

References

  1. Cohen J, Malter H, Fehilly C, Wright G, Elsner C, Kort H, et al. Implantation of embryos after partial opening of oocyte zona pellucida to facilitate sperm penetration. Lancet 1988;2:162.
  2. Cohen J. Assisted hatching of human embryos. J In Vitro Fert Embryo Transf 1991;8:179-90. https://doi.org/10.1007/BF01130802
  3. Gabrielsen A, Agerholm I, Toft B, Hald F, Petersen K, Aagaard J, et al. Assisted hatching improves implantation rates on cryopreserved-thawed embryos: a randomized prospective study. Hum Reprod 2004;19:2258-62. https://doi.org/10.1093/humrep/deh434
  4. Edi-Osagie EC, Hooper L, McGinlay P, Seif MW. Effect(s) of assisted hatching on assisted conception (IVF & ICSI). Cochrane Database Syst Rev 2003;(4):CD001894.
  5. Sallam HN, Sadek SS, Agameya AF. Assisted hatching: a metaanalysis of randomized controlled trials. J Assist Reprod Genet 2003;20:332-42. https://doi.org/10.1023/A:1024865725713
  6. Debrock S, Spiessens C, Peeraer K, De Loecker P, Willemen D, D'Hooghe TM. Higher implantation rate using modified quarter laser-assisted zona thinning in repeated implantation failure. Gynecol Obstet Invest 2009;67:127-33. https://doi.org/10.1159/000171068
  7. Hiraoka K, Hiraoka K, Horiuchi T, Kusuda T, Okano S, Kinutani M, et al. Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study. J Assist Reprod Genet 2009;26:515-21. https://doi.org/10.1007/s10815-009-9350-6
  8. Das S, Blake D, Farquhar C, Seif MM. Assisted hatching on assisted conception (IVF and ICSI). Cochrane Database Syst Rev 2009: Cd001894.
  9. Martins WP, Rocha IA, Ferriani RA, Nastri CO. Assisted hatching of human embryos: a systematic review and meta-analysis of randomized controlled trials. Hum Reprod Update 2011;17:438-53. https://doi.org/10.1093/humupd/dmr012
  10. Edirisinghe WR, Ahnonkitpanit V, Promviengchai S, Suwajanakorn S, Pruksananonda K, Chinpilas V, et al. A study failing to determine significant benefits from assisted hatching: patients selected for advanced age, zonal thickness of embryos, and previous failed attempts. J Assist Reprod Genet 1999;16:294-301. https://doi.org/10.1023/A:1020497714495
  11. Seif MM, Edi-Osagie EC, Farquhar C, Hooper L, Blake D, McGinlay P. Assisted hatching on assisted conception (IVF & ICSI). Cochrane Database Syst Rev 2006;(1):CD001894.
  12. Practice Committee of Society for Assisted Reproductive Technology; Practice Committee of American Society for Reproductive Medicine. The role of assisted hatching in in vitro fertilization: a review of the literature. A Committee opinion. Fertil Steril 2008;90:S196-8.
  13. Petersen CG, Mauri AL, Baruffi RL, Oliveira JB, Massaro FC, Elder K, et al. Implantation failures: success of assisted hatching with quarter-laser zona thinning. Reprod Biomed Online 2005;10: 224-9. https://doi.org/10.1016/S1472-6483(10)60944-3
  14. Ge HS, Zhou W, Zhang W, Lin JJ. Impact of assisted hatching on fresh and frozen-thawed embryo transfer cycles: a prospective, randomized study. Reprod Biomed Online 2008;16:589-96. https://doi.org/10.1016/S1472-6483(10)60466-X
  15. Hsieh YY, Huang CC, Cheng TC, Chang CC, Tsai HD, Lee MS. Laserassisted hatching of embryos is better than the chemical method for enhancing the pregnancy rate in women with advanced age. Fertil Steril 2002;78:179-82. https://doi.org/10.1016/S0015-0282(02)03172-2
  16. Miyata H, Matsubayashi H, Fukutomi N, Matsuba J, Koizumi A, Tomiyama T. Relevance of the site of assisted hatching in thawed human blastocysts: a preliminary report. Fertil Steril 2010;94: 2444-7. https://doi.org/10.1016/j.fertnstert.2010.01.056
  17. Valojerdi MR, Eftekhari-Yazdi P, Karimian L, Ashtiani SK. Effect of laser zona pellucida opening on clinical outcome of assisted reproduction technology in patients with advanced female age, recurrent implantation failure, or frozen-thawed embryos. Fertil Steril 2008;90:84-91. https://doi.org/10.1016/j.fertnstert.2007.06.005
  18. Hammadeh ME, Fischer-Hammadeh C, Ali KR. Assisted hatching in assisted reproduction: a state of the art. J Assist Reprod Genet 2011;28:119-28. https://doi.org/10.1007/s10815-010-9495-3
  19. Veeck LL. Does the developmental stage at freeze impact on clinical results post-thaw? Reprod Biomed Online 2003;6:367-74. https://doi.org/10.1016/S1472-6483(10)61859-7
  20. Cohen J, Elsner C, Kort H, Malter H, Massey J, Mayer MP, et al. Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum Reprod 1990;5:7-13.
  21. Lyu QF, Wu LQ, Li YP, Pan Q, Liu DE, Xia K, et al. An improved mechanical technique for assisted hatching. Hum Reprod 2005;20: 1619-23. https://doi.org/10.1093/humrep/deh809
  22. Hiraoka K, Fuchiwaki M, Hiraoka K, Horiuchi T, Murakami T, Kinutani M, et al. Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study. J Assist Reprod Genet 2008;25:129-35. https://doi.org/10.1007/s10815-008-9214-5
  23. Mantoudis E, Podsiadly BT, Gorgy A, Venkat G, Craft IL. A comparison between quarter, partial and total laser assisted hatching in selected infertility patients. Hum Reprod 2001;16:2182-6. https://doi.org/10.1093/humrep/16.10.2182
  24. Blake DA, Forsberg AS, Johansson BR, Wikland M. Laser zona pellucida thinning: an alternative approach to assisted hatching. Hum Reprod 2001;16:1959-64. https://doi.org/10.1093/humrep/16.9.1959
  25. Stein A, Rufas O, Amit S, Avrech O, Pinkas H, Ovadia J, et al. Assisted hatching by partial zona dissection of human pre-embryos in patients with recurrent implantation failure after in vitro fertilization. Fertil Steril 1995;63:838-41. https://doi.org/10.1016/S0015-0282(16)57490-1
  26. Jelinkova L, Pavelkova J, Strehler E, Paulus W, Zivny J, Sterzik K. Improved implantation rate after chemical removal of the zona pellucida. Fertil Steril 2003;79:1299-303. https://doi.org/10.1016/S0015-0282(03)00260-7
  27. Rufas-Sapir O, Stein A, Orvieto R, Avrech OM, Kotler N, Pinkas H, et al. Is assisted hatching beneficial in patients with recurrent implantation failures? Clin Exp Obstet Gynecol 2004;31:110-2.
  28. Primi MP, Senn A, Montag M, Van der Ven H, Mandelbaum J, Veiga A, et al. A European multicentre prospective randomized study to assess the use of assisted hatching with a diode laser and the benefit of an immunosuppressive/antibiotic treatment in different patient populations. Hum Reprod 2004;19:2325-33. https://doi.org/10.1093/humrep/deh430
  29. Hartshorn C, Anshelevich A, Wangh LJ. Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell mouse embryos. Fertil Steril 2005;84:1547-50. https://doi.org/10.1016/j.fertnstert.2005.05.023
  30. Kanyo K, Konc J. A follow-up study of children born after diode laser assisted hatching. Eur J Obstet Gynecol Reprod Biol 2003; 110:176-80. https://doi.org/10.1016/S0301-2115(03)00215-X

Cited by

  1. Evaluation of the impact of laser-assisted hatching techniques on the hatching process of mouse blastocysts using time-lapse microscopy vol.2, pp.1, 2015, https://doi.org/10.1016/j.xfss.2020.12.004