Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Comparative Study on The Composition of Essential Oil by Supercritical Carbon Dioxide Extraction and Hydro-distillation from Chamaecyparis obtusa Leaves

편백 잎에서 추출한 정유와 초임계 이산화탄소 추출물의 성분 비교분석

  • Kim, Jae-Woo (Division of Wood Chemistry & Microbiology, Department of Forest Products, Korea Forest Research Institute) ;
  • Choi, Won-Sil (National Instrumentation Center for Environment Management, Seoul National University) ;
  • Lee, Sung-Suk (Division of Wood Chemistry & Microbiology, Department of Forest Products, Korea Forest Research Institute) ;
  • Park, Mi-Jin (Division of Wood Chemistry & Microbiology, Department of Forest Products, Korea Forest Research Institute)
  • 김우재 (국립산림과학원 임산공학부 화학미생물과) ;
  • 최원실 (서울대학교 농업생명과학대학 농생명과학공동기기원) ;
  • 이성숙 (국립산림과학원 임산공학부 화학미생물과) ;
  • 박미진 (국립산림과학원 임산공학부 화학미생물과)
  • Received : 2015.03.23
  • Accepted : 2015.05.13
  • Published : 2015.07.25
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Abstract

This study was focused on the comparison of the variations in the yield and chemical composition of Chamaecyparis obtusa leaf oil obtained under different pressure conditions of the supercritical carbon dioxide extraction (SCE), and by hydro-distillation. SCE was carried out varying the pressure in the range of 100~400 bar at $40^{\circ}C$. The chemical composition of C. obtusa leaf oils was determined by gas chromatography-mass spectroscopy (GC-MS) analysis. The maximum yield of 4.4% (relative to the initial mass of oven dry mass) was obtained in the extraction under 300 bar pressure, which was higher than that of the hydro-distillation method (1.9%). The contents of sesquiterpenes in the extracts obtained by the SCE were higher than those of the essential oils of C. obtusa by the hydro-distillation. The sesquiterpenes in the SCE extracts made up approximately 39%~46% of the total, followed by monoterpenes, diterpene, and lignan. The contents of each constituent in the supercritical carbon dioxide extracts were varied on the extraction pressure. Therefore, these results showed that the extraction condition of SCE had significant effect on the yield of C. obtusa oils and its chemical composition.

본 연구는 초임계 이산화탄소 추출법과 증류법에 의해 얻어진 편백 정유의 수율과 화학적 조성의 변화를 비교분석 하였다. 초임계 이산화탄소 추출은 $40^{\circ}C$, 100~400 bar의 압력범위에서 수행하였다. 편백 정유의 성분 분석은 GC-MS를 이용하여 수행하였다. 초임계 이산화탄소 추출 시 300 bar 압력에서 4.4%의 최대수율을 얻었으며 이는 증류법에 의해 얻어진 1.9% 수율에 비해 높았다. 초임계 이산화탄소 추출에 의해 얻어진 추출물은 증류법에 의한 정유에 비해 sesquiterpene류의 함량이 높았다. Sesquiterpene류는 초임계 이산화탄소 추출물의 약 39%~46%를 구성하고 있으며 monoterpenes, diterpene, lignan순으로 함유되어 있다. 추출압력에 따라 각 성분의 함유율은 차이를 보였다. 따라서 초임계 이산화탄소 추출조건은 편백 정유의 수율과 함유성분에 중요한 영향을 주는 것으로 보인다.

Keywords

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