Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Fast Determination of Multiple-Reaction Intermediates for Long-Chain Dicarboxylic Acid Biotransformation by Gas Chromatography-Flame Ionization Detector

  • Cho, Yong-Han (Department of Bio and Fermentation Convergence Technology, Kookmin University) ;
  • Lee, Hye-Jin (Department of Bio and Fermentation Convergence Technology, Kookmin University) ;
  • Lee, Jung-Eun (Department of Bio and Fermentation Convergence Technology, Kookmin University) ;
  • Kim, Soo-Jung (Center for Food and Bioconvergence, Seoul National University) ;
  • Park, Kyungmoon (Department of Bio and Chemical Engineering, Hongik University) ;
  • Lee, Do Yup (Department of Bio and Fermentation Convergence Technology, Kookmin University) ;
  • Park, Yong-Cheol (Department of Bio and Fermentation Convergence Technology, Kookmin University)
  • Received : 2015.02.11
  • Accepted : 2015.02.15
  • Published : 2015.05.28
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Abstract

For the analysis of multiple-reaction intermediates for long-chain dicarboxylic acid biotransformation, simple and reproducible methods of extraction and derivatization were developed on the basis of gas chromatography with flame ionization detector (GC-FID) instead of mass spectrometry. In the derivatization step, change of the ratio of pyridine to MSTFA from 1:3 to 9:1 resulted in higher peak intensity (p = 0.021) and reproducibility (0.6%CV) when analyzing 32 g/l ricinoleic acid (RA). Extraction of RA and ω-hydroxyundec-9-enoic acid with water containing 100 mM Tween 80 showed 90.4-99.9% relative extraction efficiency and 2-7%CV compared with those with hydrophobic ethyl acetate. In conclusion, reduction of the pyridine content and change of the extraction solvent to water with Tween 80 provided compatible derivatization and extraction methods to GC-FID-based analysis of longchain carboxylic acids.

Keywords

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