한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제30권1호
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  • Pages.17-21
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  • 2015
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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한우의 정상 난포와 난포낭종 난포에서 Aquaporin7 발현 양상

Patterns of Aquaporin 7 Expression in Normal Follicles and Follicular Cyst Follicles of Hanwoo

  • 김창운 (삼성창원병원 산부인과) ;
  • 한신규 (부산대학교 분자생물학과) ;
  • 최창용 (국립축산과학원 가축유전자원센터)
  • Kim, Chang-Woon (Department of Obstetrics and Gynecology, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine) ;
  • Han, Sunkyu (Department of Molecular Biology, Pusan National University) ;
  • Choe, Changyong (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 투고 : 2015.02.04
  • 심사 : 2015.03.18
  • 발행 : 2015.03.31
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초록

Alteration in ion channel or transporter expression levels affects cell volume which is produced by movement of water and ion across the plasma membrane. In particular, aquaporin (AQP) channels among ion channels play a crucial role in movement of water across the cell membrane. This study was performed to identify whether AQP expression is changed in bovine follicular cystic follicles using microarray, RT-PCR and Western blotting analyses. In microarray data, AQP4 expression was decreased, whereas AQP7 was increased in cystic follicles. Additional experiments were focused on the AQP7 expression increased in cystic follicles. The microarray data was confirmed by semi-quantitative polymerase chain reaction (PCR) and Western blot analysis. AQP7 mRNA and protein expressions were significantly increased in the cystic follicles (p<0.05). Application of estrogen ($10{\mu}g/ml$) to bovine ovarian cells showed a trend of increase in AQP7 expression. From these results, we suggest that the increase in AQP7 expression in cystic follicles may play an important role in movement of water in bovine ovary. In addition, AQP7, a aquaglyceroporin permeating water and glycerol, could be a good target in development of methods for the cryopreservation of bovine ovary.

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참고문헌

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