Mycobiology

The Korean Society of Mycology (한국균학회)
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Acetyl Eburicoic Acid from Laetiporus sulphureus var. miniatus Suppresses Inflammation in Murine Macrophage RAW 264.7 Cells

  • Saba, Evelyn (Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University) ;
  • Son, Youngmin (Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University) ;
  • Jeon, Bo Ra (Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Seong-Eun (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Lee, In-Kyoung (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Rhee, Man Hee (Laboratory of Veterinary Physiology and Cell Signaling, Cardiovascular Research Institute and College of Veterinary Medicine, Kyungpook National University)
  • Received : 2015.02.09
  • Accepted : 2015.04.08
  • Published : 2015.06.30
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Abstract

The basidiomycete Laetiporus sulphureus var. miniatus belongs to the Aphyllophorales, Polyporaceae, and grows on the needleleaf tree. The fruiting bodies of Laetiporus species are known to produce N-methylated tyramine derivatives, polysaccharides, and various lanostane triterpenoids. As part of our ongoing effort to discover biologically active compounds from wood-rotting fungi, an anti-inflammatory triterpene, LSM-H7, has been isolated from the fruiting body of L. sulphureus var. miniatus and identified as acetyl eburicoic acid. LSM-H7 dose-dependently inhibited the NO production in RAW 264.7 cells without any cytotoxicity at the tested concentrations. Furthermore it suppressed the production of proinflammatory cytokines, mainly inducible nitric oxide synthase, cyclooxygenase-2, interleukin (IL)-$1{\beta}$, IL-6 and tumor necrosis factor ${\alpha}$, when compared with glyceraldehyde 3-phosphate dehydrogenase. These data suggest that LSM-H7 is a crucial component for the anti-inflammatory activity of L. sulphureus var. miniatus.

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References

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