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Cell Death and Proliferation after Treatment and Reinfection of Clonorchis sinensis in the Sprague-Dawley Rat Bile Duct

  • Min, Byoung-Hoon (Xenotransplantation Research Center, Seoul National University College of Medicine) ;
  • Ahn, Ka-Young (Department of Parasitology and the Institute of Travel Medicine, Korea University College of Medicine) ;
  • Lee, Haeng-Sook (Department of Parasitology and the Institute of Travel Medicine, Korea University College of Medicine) ;
  • Kim, Soo-Jin (Department of Life Science, College of Natural Science, Hallym University) ;
  • Joo, Kyoung-Hwan (Department of Parasitology and the Institute of Travel Medicine, Korea University College of Medicine)
  • Received : 2015.04.14
  • Accepted : 2015.05.13
  • Published : 2015.06.30

Abstract

The structural change and distribution of mitochondrial enzyme (ATPase, cytochrome-c-oxidase), cell proliferation (proliferating cell nuclear antigen, PCNA), cell death (caspase-3) and cell growth factor (fibroblast growth factor 8, FGF-8) in the Sprague-Dawley rat bile duct during Clonorchis sinensis infection was investigated. Experimental groups were divided into C. sinensis infection, superinfection and reinfection of C. sinensis after 'praziquantel' treatment group. As a result, C. sinensis infected rat bile ducts showed the features of chronic clonorchiasis, i.e., connective tissue thickening, ductal fibrosis and epithelial tissue dilatation. PCNA for cell proliferation increased in the infection group, and decreased after praziquantel treatment. Caspase-3 was distributed in reinfection group only. FGF-8 was distributed in the rat bile duct after praziquantel treatment but not distributed in infection and reinfection group. Overall, C. sinensis infection causes physical and chemical irritations and then brings on the abnormalities of intracellular energy metabolism and cellular growth factors, which hinders bile duct tissue from functioning properly, and resultingly, fibrosis occurs and epithelial cells dilated abnormally. More intense infection makes tissue fibrosis chronical and activates apoptosis factors.

Keywords

References

  1. Alpini G, Lenzi R, Zhai W R, Slott P A, Liu M H, Sarkozi L, and Tavoloni N (1989) Bile secretory function of intrahepatic biliary epithelium in the rat. Am. J. Physiol. 257, 124-133.
  2. Bacchi C E and Gown A M (1993) Detection of cell proliferation in tissue sections. Braz. J. Med. Biol. Res. 26, 677-687.
  3. Beaussier M, Wendum D, Fouassier L, Rey C, Barbu V, Lasnier E, Lienhart A, Scoazec J Y, Rosmorduc O, and Housset C (2005) Adaptative bile duct proliferative response in experimental bile duct ischemia. J. Hepatol. 42, 257-265.
  4. Bhamarapravati N, Thamavit W, and Vajrasthira S (1978) Liver changes in hamsters infected with a liver fluke of man, Opisthorchis viverini. Am. J. Trop. Med. Hyg. 27, 787-794. https://doi.org/10.4269/ajtmh.1978.27.787
  5. Bulut S, Uslu H, Ozdemir B, and Bulut O (2006) Expression of caspase-3, p53 and Bcl-2 in generalized aggressive periodontitis. Head Face Med. 2, 17. https://doi.org/10.1186/1746-160X-2-17
  6. Bustamante J, Di L E, Fernandez-Cobo M, Monti N, Cadenas E, and Boveris A (2004) Kinetic analysis of thapsigargin-induced thymocyte apoptosis. Free Radic. Biol. Med. 37, 1490-1498. https://doi.org/10.1016/j.freeradbiomed.2004.06.038
  7. Cha S H, Lee J H, and Rim H J (1991) Histopathological changes of the bile duct in the experimental anivals by the superinfection of Clonorchis sinensis. Korea Univ. Med. J. 28, 741-757.
  8. Choi D I, Hong S T, Li S, Chung B S, Lim J H, and Lee S H (2004) Bile duct changes in rats reinfected with Clonorchis sinensis. Korean J. Parasitol. 42, 7-17. https://doi.org/10.3347/kjp.2004.42.1.7
  9. Clark R A (2001) Fibrin and wound healing. Ann. NY. Acad. Sci. 936, 355-367.
  10. Cottrell D A and Turnbull D M (2000) Mitochondria and ageing. Curr. Opin. Clin. Nutr. Metab. Care 3, 473-478. https://doi.org/10.1097/00075197-200011000-00009
  11. Dirks A J, Hofer T, Marzetti E, Pahor M, and Leeuwenburgh C (2006) Mitochondrial DNA mutations, energy metabolism and apoptosis in aging muscle. Ageing Res. Rev. 5, 179-195. https://doi.org/10.1016/j.arr.2006.03.002
  12. Duan W R, Garner D S, Williams S D, Funckes-Shippy C L, Spath I S, and Blomme E A (2003) Comparison of immunohistochemistry for activated caspase-3 and cleaved cytokeratin 18 with the TUNEL method for quantification of apoptosis in histological sections of PC-3 subcutaneous xenografts. J. Pathol. 199, 221-228. https://doi.org/10.1002/path.1289
  13. Duke R C, Ojcius D M, and Young J D (1996) Cell suicide in health and disease. Sci. Am. 275, 80-87.
  14. Green D and Kroemer G (1998) The central executioners of apoptosis: caspases or mitochondria? Trends. Cell Biol. 8, 267-271. https://doi.org/10.1016/S0962-8924(98)01273-2
  15. Hou P C (1995) The pathology of Clonorchis sinensis infestation of the liver. J. Pathol. Bacteriol. 70, 53-64.
  16. Kim K H, Kim C D, Lee H S, Lee S J, Jeen Y T, Chun H J, Song C W, Lee S W, Um S H, Choi J H, Ryu H S, and Hyun J H (1999) Biliary papillary hyperplasia with clonorchiasis resembling cholangio- carcinoma. Am. J. Gastroenterol. 94, 514-517. https://doi.org/10.1111/j.1572-0241.1999.887_p.x
  17. Kim S J, Lee B W, Oh J Y, and Joo K H (2007) The distribution of porin and cytochrome-c-oxidase on mitochondrial cristae on the cultured fibroblast of rat bile duct, (KSEM 38th Symposium, Daejeon).
  18. Kim S J and Min B H (2004) Ultrastructural change of the bile duct fibroblast at infected rat with Clonorchis sinensis. Korean J. Electron. Microscopy 34, 121-130.
  19. Korea Association of Health Promotion (2009) Prevalence of Intestinal Parasitic Infections in Korea, 7th Report (Korea Association of Health Promotion, Seoul).
  20. Krajewska M, Wang H G, Krajewski S, Zapata J M, Shabaik A, Gascoyne R, and Reed J C (1997) Immunohistochemical analysis of in vivo patterens of expression of CPP32 (caspase-3), a cell death protease. Cancer Res. 57, 1605-1613.
  21. Lee S H, Shim T S, and Lee S M (1978) Studies on pathological changes of the liver in albino rats infected with Clonorchis sinensis. Korean J. Parasitol. 16, 148-155. https://doi.org/10.3347/kjp.1978.16.2.148
  22. Min B H, Hong S H, Lee H S, Kim S J, and Joo K H (2010) Reduction of mitochondrial electron transferase in rat bile duct fibroblast by Clonorchis sinensis infection. Korean J. Microscopy 40, 89-99.
  23. Min H K and Soh C T (1986) The effect of a carcinogen, dimethylnitrosamin, in cholangiocarcinoma in the albino rats experimentally infected with Clonorchis sinensis metacercaria. Yonsei Rep. Trop. Med. 17, 1-10.
  24. Navarro A and Boveris A (2004a) Rat brain and liver mitochondria develop oxidative stress and lose enzymatic activities on aging. Am. J. Physiol. Regul. Integr. Comp. Physiol. 287, 1244-1249. https://doi.org/10.1152/ajpregu.00226.2004
  25. Navarro A, Gomez C, Lopez-Cepero J M, and Boveris A (2004b) Beneficial effects of moderate exercise on mice aging: survival, behavior, oxidative stress, and mitochondrial electron transfer. Am. J. Physiol. Regul. Integr. Comp. Physiol. 286, 505-511. https://doi.org/10.1152/ajpregu.00208.2003
  26. Navarro A, Gomez C, Sanchez-Pino M J, Gonzalez H, Bandez M J, Boveris A D, and Boveris A (2005) Vitamin E at high doses improves survival, neurological performance, and brain mitochondrial function in aging male mice. Am. J. Physiol. Regul. Integr. Comp. Physiol. 289, 1392-1399. https://doi.org/10.1152/ajpregu.00834.2004
  27. Navarro A, Sanchez-Pino M J, Gomez C, Peralta J L, and Boveris A (2002) Behavioral dysfunction, brain oxidative stress, and impaired mitochondrial electron transfer in aging mice. Am. J. Physiol. Regul. Integr. Comp. Physiol. 282, 985-992. https://doi.org/10.1152/ajpregu.00537.2001
  28. Onuma H, Mastui C, and Morohashi M (2001) Quantitative analysis of the proliferation of epidermal cells using a human skin organ culture system and the effect of DbcAMP using markers of proliferation (BrdU, Ki-67, PCNA). Arch. Dermatol. Res. 293, 133-138. https://doi.org/10.1007/s004030000195
  29. Paunesku T, Mittal S, Protic M, Oryhon J, Korolev S V, Joachimiak A, and Woloschak G E (2001) Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Int. J. Radiat. Biol. 77, 1007-1021. https://doi.org/10.1080/09553000110069335
  30. Rim H J (1990) Clonorchiasis in Korea. Korean J. Parasitol. 28(Suppl), 63-78. https://doi.org/10.3347/kjp.1990.28.Suppl.63
  31. Senaratne S G and Colston K W (2002) Direct effects of bisphosphonates on breast cancer cells. Breast. Cancer Res. 4, 18-23. https://doi.org/10.1186/bcr473
  32. Singer A J and Clark R A (1999) Cutaneous wound healing. N. Engl. J. Med. 341, 738-746. https://doi.org/10.1056/NEJM199909023411006
  33. Skulachev V P (2006) Bioenergetic aspects of apoptosis, necrosis and mitoptosis. Apoptosis 11, 473-485. https://doi.org/10.1007/s10495-006-5881-9
  34. Turner N and Grose R (2010) Fibroblast growth factor signalling: from development to cancer. Nat. Rev. Cancer 10, 116-129. https://doi.org/10.1038/nrc2780
  35. Watanapa P and Watanapa W B (2002) Liver fluke-associated cholangiocarcinoma. Br. J. Surg. 89, 962-970. https://doi.org/10.1046/j.1365-2168.2002.02143.x
  36. Xiao Y T, Xiang L X, and Shao J Z (2007) Bone morphogenetic protein. Biochem. Biophys. Res. Commun. 362, 550-553. https://doi.org/10.1016/j.bbrc.2007.08.045