The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Monitoring for the Resistance to Prochloraz of Fusarium Species Causing Bakanae Disease in Korea

국내 벼키다리병균의 Prochloraz 약제에 대한 저항성 변화

  • Choi, Hyo-Won (Crop Protection Division, National Academy of Agricultural Science) ;
  • Lee, Yong Hwan (Disaster Management Division, Rural Development Administration) ;
  • Hong, Sung Kee (Microbial Safety Team, National Academy of Agricultural Science) ;
  • Lee, Young Kee (Crop Protection Division, National Academy of Agricultural Science) ;
  • Nam, Young Ju (Crop Protection Division, National Academy of Agricultural Science) ;
  • Lee, Jae Guem (Crop Protection Division, National Academy of Agricultural Science) ;
  • Han, Song Hee (Crop Protection Division, National Academy of Agricultural Science)
  • 최효원 (국립농업과학원 작물보호과) ;
  • 이용환 (농촌진흥청 재해대응과) ;
  • 홍성기 (국립농업과학원 유해생물팀) ;
  • 이영기 (국립농업과학원 작물보호과) ;
  • 남영주 (국립농업과학원 작물보호과) ;
  • 이재금 (국립농업과학원 작물보호과) ;
  • 한송희 (국립농업과학원 작물보호과)
  • Received : 2015.05.04
  • Accepted : 2015.06.18
  • Published : 2015.06.30
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Abstract

To investigate the changes of the resistance to prochloraz of Fusarium species causing bakanae disease, Fusarium isolates were collected from various regions in Korea, and pathogenicity tests were performed using rice seeds. Minimum inhibitory concentration (MIC) and effective concentration of 50% ($EC_{50}$) values of isolates were determined using the agar dilution method. High frequency distribution of MIC values of prochloraz against isolates collected in 2006~2007 and 2013~2014 years were $3.125{\sim}6.25{\mu}g/mL$ and $6.25{\sim}12.5{\mu}g/mL$, respectively. The mean $EC_{50}$ value of isolates increased from $0.3142{\mu}g/mL$ in 2006~2007 to $0.8124{\mu}g/mL$ in 2013~2014. Based on the $EC_{50}$ value of isolates collected in 2006~2007, the resistant baseline of prochloraz was determined as $0.6{\mu}g/mL$. Compared with the ratio of resistant isolates in 2006~2007, the ratio of resistant isolates in 2013~2014 increased from 6.5% to 41.6%.

국내에 분포하는 벼키다리병균의 prochloraz에 대한 저항성 정도를 조사하기 위하여 연도별로 균주를 수집하고, 병원성을 검정하였으며, 한천희석법을 사용하여 각 균주의 prochloraz에 대한 minimum inhibitory concentration (MIC) 값과 effective concentration of 50% ($EC_{50}$) 값을 조사하였다. 그 결과 2006년부터 2007년 분리균의 MIC 값은 $3.125{\sim}6.25{\mu}g/mL$인 균주가 가장 높은 빈도로 분포하였고, 2013년부터 2014년 분리균의 MIC 값은 $6.25{\sim}12.5{\mu}g/mL$의 농도에서 분포 비율이 가장 높았다. 또한 2006년부터 2007년 분리 균주의 평균 $EC_{50}$값은 $0.3142{\mu}g/mL$이었으며, 2013년부터 2014년 분리 균주는 $0.8124{\mu}g/mL$이었다. 2006년부터 2007년 분리 균주의 prochloraz에 대한 저항성 기준 $EC_{50}$값은 $0.6{\mu}g/mL$로 하는 것이 타당한 것으로 생각된다. 이 기준에 의한 저항성 균주의 비율은 2006년부터 2007년 분리 균주는 약 6.5%였고, 2013년부터 2014년 분리 균주에서는 41.6%로 저항성 균주의 빈도가 크게 증가한 것으로 나타났다.

Keywords

References

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