미생물학회지 (Korean Journal of Microbiology)

  • 제51권2호
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  • Pages.148-155
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  • 2015
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지
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16S rRNA 기초 새송이 버섯(Pleurotus eryngii)의 식용가능 부위 내생세균 군집 다양성

Molecular diversity of endobacterial communities in edible part of King oyster mushroom (Pleurotus eryngii) based on 16S rRNA

  • Lee, Choung Kyu (Department of Forest Resources, Gyeongnam National University of Science and Technology) ;
  • Haque, Md. Azizul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Choi, Byoung Rock (Department of Future Farming, Sacheon Agricultural Development & Technology Center) ;
  • Lee, Hee Yul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Hwang, Chung Eun (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Ahn, Min Ju (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
  • 투고 : 2014.12.17
  • 심사 : 2015.06.10
  • 발행 : 2015.06.30
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초록

세균 16S rRNA 유전자 특이 프라이머를 기초로 하여 중합 효소연쇄반응을 이용하여 Pleurotus eryngii (큰느타리 버섯)의 내부에 존재하는 세균의 다양성을 조사하였다. 세균 16S rRNA의 라이브러리는 버섯 갓(BC, body cap)와 줄기(BS, body stipe)로 구성하였다. BC 20 클론은 네 그룹으로 구별되었고 가장 큰 그룹은 Firmicutes (클론의 40%) 있었다. 그러나, BS 20 클론은 여섯 그룹으로 나뉘어졌고 가장 큰 그룹은 Actinobacteria (클론의 40%) 있었다. 전체 버섯 내에 존재하는 세균 그룹은 그람양성 세균(62.5%) 있었다.

The diversity of endobacteria in the edible part (cap and stipe) king oyster mushroom (Pleurotus eryngii) was investigated using 16S rRNA sequence analysis. The bacterial 16S rRNA libraries were constructed from the body cap (BC) and the body stipe (BS) of the king oyster mushroom. The twenty sequenced BC clones were divided into four groups and the largest group was affiliated with the Firmicutes (40% of clones). While, the twenty sequenced BS clones could be divided into six groups and the largest group was affiliated with the Actinobacteria (40% of clones). The predominant bacterial family from both the cap and stipe of the mushroom was corresponded with the Gram positive bacteria (62.5%).

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