Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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Comparison of Gangji-hwan-1, 2, 3, 4 and Combination of Gangji-hwan-1 and Gamisoche-hwan in the Reducing Effects of Body Weight in a High Fat Diet-Fed Obese Mice

고지방식이 비만마우스 모델에서 파키스탄산 및 중국산 마황으로 조성된 강지환(降脂丸)-1, 2, 3, 4와 강지환(降脂丸)-1합가미소체환(合加味消滯丸)의 체중감량효과 비교

  • Yoo, Jae Sang (Bonghwang Korean Medicine Clinic) ;
  • Ku, Ja Ryong (Soricheong Luck Korean Medicine Clinic) ;
  • Yoon, Ki Hyeon (Department of Formula Sciences and Research Institute of Korean Medicine for Diabetes and Obesity, College of Korean Medicine, Dong-Eui University,) ;
  • Jo, Ju Heum (Woosim Korean Medicine Clinic) ;
  • Jang, Du Hyon (Chungnam Korean Medicine Clinic) ;
  • Jung, Yang Sam (Jungyangsam Korean Medicine Clinic) ;
  • Kim, Jong Hoon (Neungin Korean Medicine Clinic) ;
  • Kim, Byeong Chul (Goryo Korean Medicine Clinic) ;
  • Seok, Hoa Jun (Soojung Korean Medicine Clinic) ;
  • Yoon, Michung (Department of Medical Engineering and Health Management, College of Science & Technology, MokWon University) ;
  • Roh, Jong Seong (Department of Formula Sciences and Research Institute of Korean Medicine for Diabetes and Obesity, College of Korean Medicine, Dong-Eui University,) ;
  • Shin, Soon Shik (Department of Formula Sciences and Research Institute of Korean Medicine for Diabetes and Obesity, College of Korean Medicine, Dong-Eui University,)
  • 유재상 (봉황한의원) ;
  • 구자룡 (소리청한의원) ;
  • 윤기현 (동의대학교 한의과대학 방제학교실 및 한방당뇨비만연구소) ;
  • 조주흠 (우심한의원) ;
  • 장두현 (청남한의원) ;
  • 정양삼 (정양삼한의원) ;
  • 김종훈 (능인한의원) ;
  • 김병출 (고려한의원) ;
  • 석화준 (수정한의원) ;
  • 윤미정 (목원대학교 테크노과학대학 의생명.보건학부) ;
  • 노종성 (동의대학교 한의과대학 방제학교실 및 한방당뇨비만연구소) ;
  • 신순식 (동의대학교 한의과대학 방제학교실 및 한방당뇨비만연구소)
  • Received : 2015.05.11
  • Accepted : 2015.05.28
  • Published : 2015.06.30
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Abstract

Objectives: This study was investigated the improvement effects of Pakistani Ephedra herba-containing Gangji-hwan-1, 2, 3 (Di-fatty; DF-1, 2, 3), Chinese Ephedra herba-containing Gangjihwan-4 (DF-4) and combination of DF-1 and Gamisoche-hwan (GSH) on obesity in a high fat diet-fed obese mouse model. Methods: Eight-week-old C57BL/6N mice were divided into seven groups: a normal lean group given a standard diet, an obese control group given a high fat diet, and DF-1, 2, 3, 4, and DF-1+GSH groups given a high fat diet with DF-1, 2, 3, 4 (40, 80, 160, 80 mg/kg), and DF-1+ GSH (80 mg/kg), respectively. After 8 weeks of treatment, body weight gain, feeding efficiency ratio (FER), blood lipid markers, liver histology, and fat weight and histology were examined. Results: Body weight gain was significantly decreased in DF and DF-1+GSH groups compared with control. The extent of decreases was eminent in DF-1+GSH group. FER and circulating concentration of leptin were decreased in DF and DF-1+GSH groups compared with control. Circulating concentrations of triglyceride, glucose and insulin were decreased in DF and DF-1+GSH groups compared with control. The size of adipocytes were decreased by DF and DF-1+GSH groups compared with control, whereas the adipocyte number per unit area was increased by them, suggesting that DF and DF-1+GSH groups decreased the number of large adipocytes. Conclusions: In conclusion, these results suggest that DF and DF-1+GSH groups decrease FER, plasma leptin concentration, blood anti-obesity biomarkers and fat mass, improves body weight gain. In addition, these effects were more effective in DF-1+GSH combination group than in DF-1, 2, 3, 4 groups.

Keywords

References

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