Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antioxidant and Anti-wrinkling Effects of Extracts from Vitex trifolia L.

만형자 추출물의 항산화 및 주름개선 효과

  • Lee, Mi-Kyoung (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Kim, Dong-Hee (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Park, Tae-Soon (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Son, Jun-Ho (Korea Promotion Institute for Traditional Medicine Industry)
  • Received : 2015.01.15
  • Accepted : 2015.02.13
  • Published : 2015.06.30
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Abstract

In this study, the antioxidant and anti-wrinkling effects of fraction from Vitex trifolia L. were investigated. Among the fractions, ethyl acetate fraction showed the highest antioxidant activities in 1-1-diphenyl-2-picryl-hydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoine-6-sulfonic acid) radical scavenging and elastase inhibition with 76, 89, and 74%, respectively, at a concentration of $1,000{\mu}g/mL$. This fraction, at the concentration of $25{\mu}g/mL$, inhibited 70% fibroblast cell viability and 86% the matrix metalloprotease (MMP)-1. In addition, the results from Western blot assay showed that this fraction ($25{\mu}g/mL$) expressed the MMP-1 protein level by decreasing 50%. The findings suggest that the ethyl acetate fraction from V. trifolia has great potential as a cosmeceutical ingredient with antioxidant and anti-wrinkling effects.

본 연구에서는 만형자 분획물의 항산화 및 주름개선 효과에 대하여 알아보고자 하였다. 만형자 ethyl acetate 분획물은 DPPH, ABTS에서 우수한 항산화 활성을 나타내었으며, 주름개선 효과를 검증하기 위하여 elastase 저해활성을 측정한 결과 $1,000{\mu}g/mL$에서 74% 저해활성을 나타내었다. 만형자 ethyl acetate 분획물의 세포생존율을 확인하기 위하여 섬유아세포로 MTT assay에 의해 확인 한 결과 5, 10, 15, 20, $25{\mu}g/mL$의 농도구간에서 100%에 가까운 세포 생존율을 확인하였다. 만형자 ethyl acetate 분획물의 MMP-1 저해활성을 측정 한 결과 $25{\mu}g/mL$에서 14%의 저해효과를 나타내었으며, 만형자 ethyl acetate 분획물의 MMP-1의 단백질 발현 억제 효과를 확인한 결과 $25{\mu}g/mL$의 농도에서 50% 감소하였다. 이러한 결과로 보아 만형자 ethyl acetate 분획물의 주름개선 효과를 확인할 수 있으며, 천연 기능성 화장품 소재로서의 가능성을 확인하였다.

Keywords

References

  1. Aoshima H, Tsunoue H, Koda H, and Kiso Y (2004) Aging of whiskey increases 1,1 diphenyl-2picrylhydrazyl radical scavenging activity. J Agric Food Chem 52, 5240-4. https://doi.org/10.1021/jf049817s
  2. Blois MS (1958) Antioxidant determinations by the use or a stable free radical. Nature 181, 1199-200. https://doi.org/10.1038/1811199a0
  3. Brenneisen P, Sies H, and Scharffetter-Kochanek K (2002) Ultraviolet-B irradiation and matrix metalloproteinases:from induction via signaling to initial events. Ann N Y Acad Sci 973, 31-43. https://doi.org/10.1111/j.1749-6632.2002.tb04602.x
  4. Cannell RJ, Kellam SJ, Owsianka AM, and Walker JM (1998) Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med 54, 10-4.
  5. Emerit I (1992) Free radicals and aging of the skin. In Free Radicals and Aging, Emerit I and Chance B (eds), pp. 328-41, Experientia Supplementum, Birkhauser Basel, Switzerland.
  6. Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, and Kang S (1996) Molecular basis of sun-induced premature ageing and retinoid antagonism. Nature 379, 335-9. https://doi.org/10.1038/379335a0
  7. Gilchrest BA (1990) Skin aging and photoaging. Dermatol Nurs 2, 79-82.
  8. Gross J and Lapiere CM (1962) Collagenolytic activity in amphibian tissues:a tissue culture assay. Proc Natl Acad Sci USA 54, 1197-204.
  9. Ha TY (2006) Development of functional food materials for healthy life. Korean J Crop Sci 51, 26-39.
  10. Jeong JW, Lee YC, Jung SW, and Lee KM (1994) Flavor components of citron juice as affected by the extraction method. Korean J Food Sci Technol 26, 709-12.
  11. Kang SS and Kim SJ (1994) Phytochemical Analysis of Viticis Fructus. Korean J Pharmaco gn 98, 214-20.
  12. Kim BY, Kim TG, Kang WY, Baek H, Cheon HY, and Kim D (2010) Functional cosmetic effect of porcine placenta. Korean Chem Eng Res 48, 327-31.
  13. Kim HK, Kim YE, Do JR, Lee YC, and Lee BY (1995) Antioxidative activity and physiological activity of some Korean medicinal plants. Korean J Food Sci Technol 27, 80-5.
  14. Kli gman D (2000) Cosmeveuticals. Dermatol Clin 18, 609-15. https://doi.org/10.1016/S0733-8635(05)70211-4
  15. Maeda K and Fukada M (1991) In vitro effectiveness of several whitening cosmetic components in human melanocytes. J Soc Cosmet Chem 42, 361-8.
  16. Nagase H and Woessner JF (1999) Matrix metalloproteinases. J Biol Chem 274, 21491-4. https://doi.org/10.1074/jbc.274.31.21491
  17. Ono M, Sawamura H, Ito Y, and Mizuki K (2000) Diterpenoids from the fruits of Vitex trifolia. Phytochemistry 55, 873-7. https://doi.org/10.1016/S0031-9422(00)00214-4
  18. Park JH and Lee CK (2000) The Encyclopedia of Medicinal Plants. Shinilbooks. Korea.
  19. Philips N, Keller T, Hendrix C, Hamilton S, Arena R, Tuason M et al. (2007) Regulation of the extracellular matrix remodeling by lutein in dermal fibroblasts, melanoma cells, and ultraviolet radiation exposed fibroblasts. Arch Dermatol Res 299, 373-9. https://doi.org/10.1007/s00403-007-0779-0
  20. Re R, Pellegrini N, Proteggente A, Yang M, and Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26, 1231-7. https://doi.org/10.1016/S0891-5849(98)00315-3
  21. Talwar HS, Griffiths CE, Fisher GJ, Hamilton TA, and Voorhees JJ (1995) Reduced type I and type III procollagens in photodamaged adult human skin. J Invenst Dermatol 105, 285-90. https://doi.org/10.1111/1523-1747.ep12318471
  22. Tsukahara K, Nakagawa H, Moriwaki S, Takema Y, Fujimura T, and Imokawa G (2006) Inhibition of ultraviolet-B-induced wrinkle formation by an elastase-inhibiting herbal extract: implication for the mechanism underlying elastase-associated wrinkles. Int J Dermatol 45, 460-8. https://doi.org/10.1111/j.1365-4632.2006.02557.x
  23. Xin YH, Zhang QY, and Huang BK (2006) Study on chemical constituents in fruits of Vitex trifolia L. var. simplicifolia Cham. Arch Pharm Res 29, 747-9.

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