Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지
DOI QR코드

DOI QR Code

Development of Analytical Methods of Hyperoside from Rosa canina L.

Rosa canina L. 중 hyperoside의 시험법 개발 연구

  • Oh, Jae Myoung (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Lee, Hwa Jung (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Bahn, Kyeong Nyeo (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Seo, Il Won (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Lee, Young Joo (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Lee, Jin Hee (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Park, Ji Min (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation) ;
  • Kang, Tae Seok (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation)
  • 오재명 (식품의약품안전평가원 영양기능연구팀) ;
  • 이화정 (식품의약품안전평가원 영양기능연구팀) ;
  • 반경녀 (식품의약품안전평가원 영양기능연구팀) ;
  • 서일원 (식품의약품안전평가원 영양기능연구팀) ;
  • 이영주 (식품의약품안전평가원 영양기능연구팀) ;
  • 이진희 (식품의약품안전평가원 영양기능연구팀) ;
  • 박지민 (식품의약품안전평가원 영양기능연구팀) ;
  • 강태석 (식품의약품안전평가원 영양기능연구팀)
  • Received : 2014.12.04
  • Accepted : 2015.03.18
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Rosa canina L. is health functional food materials that can help to temporarily relieve symptoms of arthritis. This study has been conducted to develop and validate analytical methods for hyperoside of Rosa canina L.. Methods based on HPLC with ultraviolet detection (UVD) were established through instrumental analytical conditions, and the examination of data, such as domestic and foreign reliable methods and journals. HPLC UVD analysis using Capcell Pak $C_{18}$ MG II column at 353 nm was determined on test through the column, mobile phase. The validation has been performed on the method to determine linearity, accuracy, limits of quantification (LOQ) and repeatability for hyperoside. The method showed high linearity in the calibration curve at a coefficient of correlation ($R^2$) of 0.999, and the LOQ was $0.393{\mu}g/mL$. Relative standard deviation (RSD) values of data from repeatability precision was between 0.6 and 2.6%. Recovery rate test at hyperoside scored between 98 and 99%. These results indicate that the established HPLC method is very useful for the determination of hyperoside in Rosa canina L. to develop a health functional material.

본 연구에서는 일시적으로 관절염 증상 완화에 효과가 있는 건강기능식품인 Rosa Canina L.의 지표성분인 hyperoside를 분석하였다. 국내 외 분석법과 저널들을 참고하여 hyperoside의 HPLC 분석법을 확립하였다. Hyperoside의 HPLC 분석법은 이동상과 컬럼에 대한 실험을 통하여 Capcell Pak $C_{18}$ MG II 컬럼으로 353 nm으로 설정하였다. 시험 분석법 검증은 hyperoside에 대한 직선성, 정확성, 정량한계(LOQ) 및 정밀성을 수행하였다. Hyperoside은 $2{\sim}60{\mu}g/mL$ 농도에서 우수한 직선성($R^2=0.999$)을 나타내었다. 정확성의 회수율은 98~99%로 관찰되었으며, LOQ는 $0.393{\mu}g/mL$으로 나타났고, 재현성에 대한 정밀성은 상대표준편차가 0.6~2.6%로 관찰되었다.

Keywords

References

  1. Chrubasik C, Roufogalis BD, Muller-Lander U, Chrubasik S. A systematic review on the Rosa canina effect and efficacy profiles, Phytother Res. 22, 725-733 (2008). https://doi.org/10.1002/ptr.2400
  2. Basgel S, Erdemog.lu SB. Determination of mineral and trace elements in some medicinal herbs and their infusions consumed in Turkey, Sci Total Environ. 359, 82-89 (2006). https://doi.org/10.1016/j.scitotenv.2005.04.016
  3. Da-Eun Nam, Min-jae Lee, Namgil Kang, Geumduck Park, and Jeongmin Lee. A Comparative Study of Rose Hip Extracts on Osteoarthritis in Cartilage Cells, J Korean Soc Food Sci Nutr. 41(12), 1663-1670 (2012). https://doi.org/10.3746/jkfn.2012.41.12.1663
  4. Loana R, Andreea S, Sorin S. Bioactive compound and antioxidant activity of Rosa canina L. biotypes from spontaneous flora of Transylvania. Chemistry Central Journal. 7(73), 1-10 (2013). https://doi.org/10.1186/1752-153X-7-1
  5. Saeed C. A., Houshang N., Sepeideh J., Fatemeh H., Protective effect of Rosa canina L fruit extracts on renal disturbances induced by reperfusion injury in rats. Kidney diseases. 7(4), 290-298 (2013).
  6. Odd W, Sigrun S, Ewa H, Hanna M, Liv E. The effects of a standardized herbal remedy made from a subtype of Rosa canina in patients with osteoarthritis: a double blind, randomized, placebo-controlled clinical trial. Current therapeutic research. 64(1), 21-31 (2003). https://doi.org/10.1016/S0011-393X(03)00004-3
  7. Rein, E., Kharazmi, A., Winther, K., A herbal remedy, Hyben Vital (stand. Powder of a subspecies of Rosa canina fruits) reduces pain and improves general wellbeing in patients with osteoarthritis - a double-blind, placebo-controlled, randomised trial, Phytomedicine. 11, 383-391 (2004). https://doi.org/10.1016/j.phymed.2004.01.001
  8. Winther K1, Apel K, Thamsborg G. A powder made from seeds and shells of a rose-hip subspecies (Rosa canina) reduces symptoms of knee and hip osteoarthritis: a randomized, double-blind, placebo-controlled clinical trial, Scand J Rheumatol. 34(4), 302-308 (2005). https://doi.org/10.1080/03009740510018624
  9. Jager, A.K., Eldeen, I.M.S., van Staden, J., COX-1 and COX-2 activity of rose hip, Phytotherapy Research. 21, 1251-1252 (2007). https://doi.org/10.1002/ptr.2236
  10. E.M. Wenzig, U. Widowitz, O. Kunert, S. Chrubasik. Phytochemical composition and in vitro pharmacological activity of two rose hip (Rosa canina L.) preparations, Phytomedicine. 15(10), 826-835 (2008). https://doi.org/10.1016/j.phymed.2008.06.012
  11. Orhana D.D., Hartevioglu A., Kupeli E and Yesilada E., In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. Fruits, Journal of Ethnopharmacology. 112, 394-400 (2007). https://doi.org/10.1016/j.jep.2007.03.029
  12. Lattanzioa F., Grecob E., Caretta D., Cervellatib R., Govonic P. and Speronia E., In vivo anti-inflammatory effect of Rosa canina L. Extract, J. of Ethnopharmacology. 137(1), 880-885 (2011). https://doi.org/10.1016/j.jep.2011.07.006
  13. ARTUR A., WALDEMER B., JERZY Z., SEBASTIAN M., Flavonoid and organic acid content in rose hips (Rosa L., SECT. CANINAE DC. EM. CHRIST), Acta Biologica Cracoviesia Series Botanica. 54(1), 105-112 (2012).
  14. Zhengyu Z., Mosha Silas Sethiel, Weizhi Shen, Sentai Liao, Yuxiao Zou., Hyperoside Downregulates the Receptor for Advanced Glycation End Products (RAGE) and Promotes Proliferation in ECV304 Cells via the c-Jun N-Terminal Kinases (JNK) Pathway Following Stimulation by Advanced Glycation End-Products In Vitro, Int. J. Mol. Sci. 14, 22697-22707 (2013). https://doi.org/10.3390/ijms141122697
  15. Lin-lin WU, Xin-bo YANG, Zhen-ming HUANG, He-zhi LIU, Guang-xia WU, In vivo and in vitro antiviral activity of hyperoside extracted from Abelmoschus manihot (L) medik, Acta pharmacol Sin. 28(3), 404-409 (2007). https://doi.org/10.1111/j.1745-7254.2007.00510.x
  16. Kyu Hong Choe, Sun-Nam Choe, Jong-Jae Choo, Jeong-Yeol Lee, Jong-Yeon Kim, Ju-Wan Kim, Ji-Sung Choi, Ki-Seok Park, and Kwan Ha Park. Tissue Concentration of Quercetin, Isoquercitrin and Hyperoside, and Lipid Profile Changes Following 8-week Feeding of Angelica Kieskei Powder in Rats, Korean J. Food. Sci. Technol. 39(6), 721-724 (2007).
  17. available online http://www.uspnf.com
  18. ICH Harmonised tripartite guideline; Validation of analytical procedures test and methodology Q2 (R1).
  19. AOAC Guideline for Single Laboratory Validation of Chemical Methosd for Dietary Supplements and Botanicals.