Molecules and Cells

  • 제38권6호
  • /
  • Pages.506-517
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  • 2015
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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Suppression of ASKβ(AtSK32), a Clade III Arabidopsis GSK3, Leads to the Pollen Defect during Late Pollen Development

  • Dong, Xiangshu (Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Nou, Ill-Sup (Department of Horticulture, Sunchon National University) ;
  • Yi, Hankuil (Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Hur, Yoonkang (Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University)
  • 투고 : 2014.11.18
  • 심사 : 2015.02.23
  • 발행 : 2015.06.30
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초록

Arabidopsis Shaggy-like protein kinases (ASKs) are Arabidopsis thaliana homologs of glycogen synthase kinase 3/SHAGGY-like kinases (GSK3/SGG), which are comprised of 10 genes with diverse functions. To dissect the function of $ASK{\beta}$ (AtSK32), $ASK{\beta}$ antisense transgenic plants were generated, revealing the effects of $ASK{\beta}$ down-regulation in Arabidopsis. Suppression of $ASK{\beta}$ expression specifically interfered with pollen development and fertility without altering the plants' vegetative phenotypes, which differed from the phenotypes reported for Arabidopsis plants defective in other ASK members. The strength of these phenotypes showed an inverse correlation with the expression levels of $ASK{\beta}$ and its co-expressed genes. In the aborted pollen of $ASK{\beta}$ antisense plants, loss of nuclei and shrunken cytoplasm began to appear at the bicellular stage of microgametogenesis. The in silico analysis of promoter and the expression characteristics implicate $ASK{\beta}$ is associated with the expression of genes known to be involved in sperm cell differentiation. We speculate that $ASK{\beta}$ indirectly affects the transcription of its co-expressed genes through the phosphorylation of its target proteins during late pollen development.

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참고문헌

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