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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of DBD-bio-plasma on the HSP70 of Fibroblasts: A New Approach on Change of Molecular Level by Heat Shock in the Cell

Fibroblasts 세포주의 HSP70에 대한 DBD-bio-plasma의 effects: Cell에서 Heat Shock에 의한 Molecular Level 변화로의 새로운 접근법

  • Kim, Kyoung-Yeon (Department of Medicine and Health Sciences, College of Medicine, the Catholic University of Korea) ;
  • Yi, Junyeong (Department of Medicine and Health Sciences, College of Medicine, the Catholic University of Korea) ;
  • Nam, Min-Kyung (Department of Medicine and Health Sciences, College of Medicine, the Catholic University of Korea) ;
  • Choi, Eun Ha (Plasma Bioscience Research Center, Kwangwoon University) ;
  • Rhim, Hyangshuk (Department of Medicine and Health Sciences, College of Medicine, the Catholic University of Korea)
  • 김경연 (가톨릭대학교 의생명건강과학과) ;
  • 이준영 (가톨릭대학교 의생명건강과학과) ;
  • 남민경 (가톨릭대학교 의생명건강과학과) ;
  • 최은하 (광운대학교 플라즈마 바이오과학 연구센터) ;
  • 임향숙 (가톨릭대학교 의생명건강과학과)
  • Received : 2014.11.22
  • Accepted : 2015.01.20
  • Published : 2015.02.27
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Abstract

Plasma is an ionized gas mixture, consisting of neutral particles, positive ions, negative electrons, electronically excited atoms and molecules, radicals, UV photons, and various reactive species. Also, plasma has unique physical properties distinct from gases, liquids, and solids. Until now, non-thermal plasmas have been widely utilized in bio-medical applications (called bio-plasma) and have been developed for the plasma-related devices that are used in the medical field. Although numerous bio-plasma studies have been performed in biomedicine, there is no confirmation of the nonthermal effect induced by bio-plasma. Standardization of the biological application of plasma has not been evaluated at the molecular level in living cells. In this context, we investigated the biological effect of bio-plasma on living cells. Hence, we treated the fibroblasts with Dielectric Bauvier Discharge bio-plasma (DBD), and assessed the characteristic change at the molecular level, one of the typical cellular responses. Heat shock protein 70 (HSP70) regulates its own protein level in response to stimuli. HSP70 responds to heat shock by increasing its own expression at the molecular level in cells. Hence, we confirmed the level of HSP70 after treatment of mouse embryonic fibroblasts (MEFs) with DBD. Interestingly, DBD-plasma induced cell death, but there was no difference in the level of HSP70, which is induced by heat shock stimuli, in DBD-treated MEFs. Our data provide the basic information on the interaction between MEFs and DBD, and can help to design a molecular approach in this field.

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