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Streptococcus mutans biofilm에 대한 광역동 치료의 최적조건에 관한 연구

Optimum Treatment Parameters for Photodynamic Antimicrobial Chemotherapy on Streptococcus mutans Biofilms

  • 최서정 (강릉원주대학교 치과대학 소아치과학교실) ;
  • 박호원 (강릉원주대학교 치과대학 소아치과학교실) ;
  • 이주현 (강릉원주대학교 치과대학 소아치과학교실) ;
  • 서현우 (강릉원주대학교 치과대학 소아치과학교실) ;
  • 이시영 (강릉원주대학교 치과대학 미생물학 및 면역학교실 및 구강과학연구소)
  • Choi, Seojung (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Howon (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Juhyun (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University) ;
  • Seo, Hyunwoo (Department of Pediatric Dentistry, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Siyoung (Department of Microbiology and Immunology, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
  • 투고 : 2014.10.13
  • 심사 : 2015.01.20
  • 발행 : 2015.05.31

초록

할로겐 광중합기를 광원으로, 치면세균막 착색제인 erythrosine을 광감각제로 사용하여 S. mutans biofilm에 대한 광역동 치료를 시행할 때의 최적조건을 알아보기 위해서 erythrosine의 농도와 광조사 시간 및 광감각제의 접촉시간에 따른 광역동 치료의 효과를 비교하였다. erythrosine의 농도를 0, 10, 20, 40, $80{\mu}M$로 변화시킨 결과 농도가 증가할수록 항균효과가 증가하는 경향을 보였으며 $40{\mu}M$$80{\mu}M$의 두 군에서 통계적 유의성이 나타났다. 광 조사 시간을 0, 5, 15, 30, 60, 75초로 변화시킨 결과 조사 시간이 길어질수록 항균효과가 증가하는 경향을 보였으며 30초, 60초, 75초의 세 군에서 통계적 유의성이 나타났다. erythrosine 처리시간을 0분, 1분, 2분30초, 5분으로 변화시킨 결과 erythrosine과의 접촉시간이 늘어날수록 항균효과가 증가하는 경향을 보였으며 2분30초 군과 5분 군에서 통계적 유의성을 보였다. 이상의 결과로 광감각제로 사용한 erythrosine의 농도를 $20-40{\mu}M$, 광원으로 사용한 할로겐 광중합기의 광 조사시간을 30초 이상, 광감각제인 erythrosine을 2분30초 이상 처리하여 사용하는 것이 치아우식 예방을 위한 광역동 치료에 효과적임을 알 수 있다.

The purpose of this study was to evaluate the effects of Photochemotherapy using a combination of erythrosine and standard halogen dental curing lights on the viability of Streptococcus mutans in the biofilm phase. To investigate the optimum treatment parameters, the researchers controlled the concentration of erythrosine, light irradiation time and the treatment time of erythrosine. The higher concentration of erythrosine (0, 10, 20, 40, 80 M) in the presence of light irradiation created greater effects in reducing the viability of S. mutans. The results showed a statistically significant difference among the antimicrobial effects in 20, 40, 80 M erythrosine. The higher irradiation time of light (0, 5, 15, 30, 60, 75s) in the presence of erythrosine showed greater effects in reducing the viability of S. mutans. There was statistically significant difference in 30, 60, 75 seconds. The higher treatment time of erythrosine (0, 1, 2.5, 5min) in the presence of erythrosine created greater effects on reduction of S. mutans viability. Statistically significant differences were found between 2.5 and 5 minutes of erythrosine treatment time. The results of this study showed that the photochemotherapy on S. mutans using erythrosine and the halogen dental curing lights conventionally used in dental clinics is effective in the condition of 20-40 M erythrosine concentration, irradiation time over 30 seconds, and erythrosine treatment time over 2.5 minutes.

키워드

참고문헌

  1. Bomin Kwon, Ikhyun Bae, Shin Kim, et al. : Dental caries status of 14-16 year old adolescents in Yangsan area. J Korean Acad Pediatr Dent, 41:8-17, 2014. https://doi.org/10.5933/JKAPD.2014.41.1.8
  2. Loe H : Oral hygiene in the prevention of caries and periodental disease. Int Dent J, 50:129-139, 2000. https://doi.org/10.1111/j.1875-595X.2000.tb00553.x
  3. Smith DJ : Dental caries vaccines: prospects and concerns. Crit Rev Oral Biol Med, 13:335-349, 2002. https://doi.org/10.1177/154411130201300404
  4. Marsh PD : Microbiologic aspects of dental plague and dental caries. Dent Clin North Am, 43:599-614, 1999.
  5. Francisco Ramos-Gomez, Young Jae Kim, Man-Wai Ng, et al. : New visions in pediatric dentistry keeping healthy teeth caries free: Pediatric cambra protocols. J Korean Acad Pediatr Dent, 40:72-82, 2013. https://doi.org/10.5933/JKAPD.2013.40.1.72
  6. Hoyle BD, Costerton JW : Bacterial resistance to antibiotics : the role of biofilms. Prog Drug Res, 37:91-105, 1991.
  7. Ka-Young Lee, Sang-Ho Lee, Nan-Young Lee : Evaluation of fluoride-releasing capacity from polyvinyl alcohol polymer tape supplemented with NaF in oral cavity. J Korean Acad Pediatr Dent, 40:89-97, 2013. https://doi.org/10.5933/JKAPD.2013.40.2.89
  8. Hellwig E, Lennon AM : Systemic versus topical fluoride. Caries Res, 38:258-262, 2004. https://doi.org/10.1159/000077764
  9. Jongcheol Park, Howon Park, Siyoung Lee : Enhancement of erythrosine photodynamic therapy against Streptococcus mutans by chlorhexidine. J Korean Acad Pediatr Dent, 40:241-246, 2013. https://doi.org/10.5933/JKAPD.2013.40.4.241
  10. Wesley MS, Cynthia MA, Johan EL : Photodynamic therapeutics: basic principles and clinical applications. DDT, 4:507-517, 1999. https://doi.org/10.1016/S1359-6446(99)01412-9
  11. Konopka K, Goslinski T : Photodynamic therapy in dentistry. J Dent Res, 86:694-707, 2007. https://doi.org/10.1177/154405910708600803
  12. Nikolaos S. : Photodynamic therapy in the control of oral biofilms. Periodontology 2000, 55:143-166, 2011. https://doi.org/10.1111/j.1600-0757.2010.00346.x
  13. Juliana YN : Antibacterial photodynamic therapy for dental caries : Evaluation of the photosensitizers used and light source properties. Photodiagnosis and Photodynamic Therapy, 9:112-131, 2012.
  14. Zanin IC, Goncalves RB : Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study. J Antimicrob Chemother 56, 56:324-330, 2005. https://doi.org/10.1093/jac/dki232
  15. Chan Y, Lai CH : Bactericidl effects of different laser wavelengths on periodontopathic germs in photodynamic therapy. Lasers Med Sci, 18:51-55, 2003. https://doi.org/10.1007/s10103-002-0243-5
  16. Dobson J, Wilson M : Sensitization of oral bacteria in biofilms to killing by light from a low-power laser. Arch Oral Biol, 37:883-887, 1992. https://doi.org/10.1016/0003-9969(92)90058-G
  17. Giusti JS, Snatos PL : Antimicrobial photodynamic action on dentin using a light-emitting diode light source. Photomed Laser Surg, 26:281-287, 2008. https://doi.org/10.1089/pho.2007.2149
  18. Costa AC, Chibebe JJ : Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode. Braz Oral Res 24:413-418, 2010. https://doi.org/10.1590/S1806-83242010000400007
  19. Lee YH, Park HW, Lee JH, et al. : The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit. Int J Oral Sci, 4:196-201, 2012. https://doi.org/10.1038/ijos.2012.63
  20. Park MS, Park HW : Susceptibility of Streptococcus mutans to photodynamic therapy with erythrosine and dental halogen curing unit. Gangneung: Gangneung-Wonju National University; 2011.
  21. Jung JS, Park HW, Lee JH : The effect of photodynamic therapy on the viability of Streptococcus mutans isolated from oral cavity. J Korean Acad Pediatr Dent, 39:233-241, 2012. https://doi.org/10.5933/JKAPD.2012.39.3.233
  22. Dougherty TJ, Gomer CJ : Photodynamic therapy. J Natl Cancer Inst, 90:889-905, 1998. https://doi.org/10.1093/jnci/90.12.889
  23. Okamoto H, Iwase T : Dye-mediated bactercidal effect of He-Ne laser irradiation on oral microorganisms. Lasers Surg Med, 12:450-458, 1992. https://doi.org/10.1002/lsm.1900120415
  24. Marsh PD, Bevis RA, Newman HN : Antibacterial activity of some plaque-disclosing agents and dyes. Caries Res, 23:348-350, 1989. https://doi.org/10.1159/000261205
  25. Metcalf D, Robinson C, Devine D, et al. : Enhancement of erythrosine-mediated photodynamic therapy of Streptococcous mutans biofilms by light fractionation. J Antimicrob Chemother, 58:190-192, 2006. https://doi.org/10.1093/jac/dkl205
  26. Wood S, Metcalf D : Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms. J Antimicrob Chemother, 57:680-684, 2006. https://doi.org/10.1093/jac/dkl021

피인용 문헌

  1. Antimicrobial Effect on Streptococcus mutans in Photodynamic Therapy using Different Light Source vol.45, pp.1, 2018, https://doi.org/10.5933/JKAPD.2018.45.1.82