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The effect of yacon (Samallanthus sonchifolius) ethanol extract on cell proliferation and migration of C6 glioma cells stimulated with fetal bovine serum

  • Lee, Kang Pa (Department of Medical Science, School of Medicine, Konkuk University) ;
  • Choi, Nan Hee (Department of Anatomy, college of Korean Medicine, Dongguk University Gyeongju Campus) ;
  • Kim, Jin Teak (Department of Anatomy, college of Korean Medicine, Dongguk University Gyeongju Campus) ;
  • Park, In-Sik (Department of Anatomy, college of Korean Medicine, Dongguk University Gyeongju Campus)
  • 투고 : 2014.08.11
  • 심사 : 2015.01.08
  • 발행 : 2015.06.01

초록

BACKGROUND/OBJECTIVES: Yacon (Samallanthus sonchifolius), a common edible plant grown throughout the world, is well known for its antidiabetic properties. It is also known to have several other pharmacological properties including anti-inflammatory, anti-oxidant, anti-allergic, and anti-cancer effects. To date, the effect of yacon on gliomas has not been studied. In this study, we investigated the effects of yacon on the migration and proliferation of C6 glioma cells stimulated by fetal bovine serum (FBS). MATERIALS/METHODS: Cell growth and proliferation were determined by evaluating cell viability using an EZ-Cytox Cell Viability Assay Kit. FBS-induced migration of C6 glioma cells was evaluated by performing the scratch wound healing assay and the Boyden chamber assay. We also used western blot analysis to determine the expression levels of extracellular signal-regulated kinase 1/2 (ERK1/2), a major regulator of migration and proliferation of glioma cells. Matrix metallopeptidase (MMP) 9 and TIMP-1 levels were measured by performing reverse transcription PCR. RESULTS: Yacon ($300{\mu}g/mL$) reduced both the FBS-induced proliferation of C6 glioma cells and the dose-dependent migration of the FBS-stimulated C6 cells. FBS-stimulated C6 glioma cells treated with yacon (200 and $300{\mu}g/mL$) showed reduced phosphorylation of ERK1/2 and inhibition of MMP 9 expression compared to those shown by the untreated FBS-stimulated C6 cells. In contrast, yacon (200 and $300{\mu}g/mL$) induced TIMP-1 expression. CONCLUSIONS: On the basis of these results, we suggest that yacon may exert an anti-cancer effect on FBS-stimulated C6 glioma cells by inhibiting their proliferation and migration. The most likely mechanism for this is down-regulation of ERK1/2 and MMP9 and up-regulation of TIMP-1 expression levels.

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참고문헌

  1. Chinot OL, Macdonald DR, Abrey LE, Zahlmann G, Kerloeguen Y, Cloughesy TF. Response assessment criteria for glioblastoma: practical adaptation and implementation in clinical trials of antiangiogenic therapy. Curr Neurol Neurosci Rep 2013;13:347. https://doi.org/10.1007/s11910-013-0347-2
  2. Hess KR, Broglio KR, Bondy ML. Adult glioma incidence trends in the United States, 1977-2000. Cancer 2004;101:2293-9. https://doi.org/10.1002/cncr.20621
  3. Marucci G. The effect of WHO reclassification of necrotic anaplastic oligoastrocytomas on incidence and survival in glioblastoma. J Neurooncol 2011;104:621-2. https://doi.org/10.1007/s11060-010-0523-z
  4. Coniglio SJ, Segall JE. Review: molecular mechanism of microglia stimulated glioblastoma invasion. Matrix Biol 2013;32:372-80. https://doi.org/10.1016/j.matbio.2013.07.008
  5. Roy LO, Poirier MB, Fortin D. Transforming growth factor-beta and its implication in the malignancy of gliomas. Target Oncol. Forthcomng 2014.
  6. Strniskova M, Barancik M, Ravingerova T. Mitogen-activated protein kinases and their role in regulation of cellular processes. Gen Physiol Biophys 2002;21:231-55.
  7. Chang L, Karin M. Mammalian MAP kinase signalling cascades. Nature 2001;410:37-40. https://doi.org/10.1038/35065000
  8. Park SL, Kim WJ, Moon SK. p21WAF1 mediates the IL-15-induced migration and invasion of human bladder cancer 5637 cells via the ERK1/2/NF-kappaB/MMP-9 pathway. Int Immunopharmacol 2014;22: 59-65. https://doi.org/10.1016/j.intimp.2014.06.008
  9. Khasigov PZ, Podobed OV, Gracheva TS, Salbiev KD, Grachev SV, Berezov TT. Role of matrix metalloproteinases and their inhibitors in tumor invasion and metastasis. Biochemistry (Mosc) 2003;68: 711-7. https://doi.org/10.1023/A:1025051214001
  10. Fang W, Li H, Kong L, Niu G, Gao Q, Zhou K, Zheng J, Wu B. Role of matrix metalloproteinases (MMPs) in tumor invasion and metastasis: serial studies on MMPs and TIMPs. Beijing Da Xue Xue Bao 2003;35:441-3.
  11. Deryugina EI, Quigley JP. Matrix metalloproteinases and tumor metastasis. Cancer Metastasis Rev 2006;25:9-34. https://doi.org/10.1007/s10555-006-7886-9
  12. Delgado GT, Tamashiro WM, Marostica Junior MR, Pastore GM. Yacon (Smallanthus sonchifolius): a functional food. Plant Foods Hum Nutr 2013;68:222-8. https://doi.org/10.1007/s11130-013-0362-0
  13. Genta S, Cabrera W, Habib N, Pons J, Carillo IM, Grau A, Sanchez S. Yacon syrup: beneficial effects on obesity and insulin resistance in humans. Clin Nutr 2009;28:182-7. https://doi.org/10.1016/j.clnu.2009.01.013
  14. de Almeida Paula HA, Abranches MV, de Luces Fortes Ferreira CL. Yacon (Smallanthus sonchifolius): a food with multiple functions. Crit Rev Food Sci Nutr 2015;55:32-40. https://doi.org/10.1080/10408398.2011.645259
  15. Piette C, Deprez M, Roger T, Noel A, Foidart JM, Munaut C. The dexamethasone-induced inhibition of proliferation, migration, and invasion in glioma cell lines is antagonized by macrophage migration inhibitory factor (MIF) and can be enhanced by specific MIF inhibitors. J Biol Chem 2009;284:32483-92. https://doi.org/10.1074/jbc.M109.014589
  16. Kobayashi T, Hattori S, Shinkai H. Matrix metalloproteinases-2 and -9 are secreted from human fibroblasts. Acta Derm Venereol 2003;83:105-7. https://doi.org/10.1080/00015550310007436
  17. Delgado GT, Thome R, Gabriel DL, Tamashiro WM, Pastore GM. Yacon (Smallanthus sonchifolius)-derived fructooligosaccharides improves the immune parameters in the mouse. Nutr Res 2012;32:884-92. https://doi.org/10.1016/j.nutres.2012.09.012
  18. Lin F, Hasegawa M, Kodama O. Purification and identification of antimicrobial sesquiterpene lactones from yacon (Smallanthus sonchifolius) leaves. Biosci Biotechnol Biochem 2003;67:2154-9. https://doi.org/10.1271/bbb.67.2154
  19. Martin V, Herrera F, Garcia-Santos G, Antolin I, Rodriguez-Blanco J, Rodriguez C. Signaling pathways involved in antioxidant control of glioma cell proliferation. Free Radic Biol Med 2007;42:1715-22. https://doi.org/10.1016/j.freeradbiomed.2007.02.028
  20. Campos D, Betalleluz-Pallardel I, Chirinos R, Aguilar-Galvez A, Noratto G, Pedreschi R. Prebiotic effects of yacon (Smallanthus sonchifolius Poepp. & Endl), a source of fructooligosaccharides and phenolic compounds with antioxidant activity. Food Chem 2012;135:1592-9. https://doi.org/10.1016/j.foodchem.2012.05.088
  21. Friedl P, Wolf K. Tumour-cell invasion and migration: diversity and escape mechanisms. Nat Rev Cancer 2003;3:362-74. https://doi.org/10.1038/nrc1075
  22. Nagano M, Hoshino D, Koshikawa N, Akizawa T, Seiki M. Turnover of focal adhesions and cancer cell migration. Int J Cell Biol 2012; 2012:310616.
  23. Gialeli C, Theocharis AD, Karamanos NK. Roles of matrix metalloproteinases in cancer progression and their pharmacological targeting. FEBS J 2011;278:16-27. https://doi.org/10.1111/j.1742-4658.2010.07919.x
  24. Li C, Wang F, Liu L. Ultra-high performance liquid chromatography-mass spectrometry for analysis of newborn and fetal bovine serum components. Nan Fang Yi Ke Da Xue Xue Bao 2014;34:751-3.
  25. Wang M, Maier P, Wenz F, Giordano FA, Herskind C. Mitogenic signalling in the absence of epidermal growth factor receptor activation in a human glioblastoma cell line. J Neurooncol 2013;115:323-31. https://doi.org/10.1007/s11060-013-1232-1
  26. Ito Y, Correll K, Schiel JA, Finigan JH, Prekeris R, Mason RJ. Lung fibroblasts accelerate wound closure in human alveolar epithelial cells through hepatocyte growth factor/c-Met signaling. Am J Physiol Lung Cell Mol Physiol 2014;307:L94-105. https://doi.org/10.1152/ajplung.00233.2013
  27. Lin MC, Huang MJ, Liu CH, Yang TL, Huang MC. GALNT2 enhances migration and invasion of oral squamous cell carcinoma by regulating EGFR glycosylation and activity. Oral Oncol 2014;50:478-84. https://doi.org/10.1016/j.oraloncology.2014.02.003
  28. Dong XZ, Huang CL, Yu BY, Hu Y, Mu LH, Liu P. Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K pathways in C6 glioma cells. Phytomedicine 2014;21:1178-88. https://doi.org/10.1016/j.phymed.2014.04.022

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