Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Cell Proliferation and Motility Are Inhibited by G1 Phase Arrest in 15-kDa Selenoprotein-Deficient Chang Liver Cells

  • Bang, Jeyoung (School of Biological Sciences, Seoul National University) ;
  • Huh, Jang Hoe (School of Biological Sciences, Seoul National University) ;
  • Na, Ji-Woon (School of Biological Sciences, Seoul National University) ;
  • Lu, Qiao (School of Biological Sciences, Seoul National University) ;
  • Carlson, Bradley A. (Mouse Cancer Genetics Program, National Cancer Institute, National Institutes of Health) ;
  • Tobe, Ryuta (Mouse Cancer Genetics Program, National Cancer Institute, National Institutes of Health) ;
  • Tsuji, Petra A. (Department of Biological Sciences, Towson University) ;
  • Gladyshev, Vadim N. (Brigham and Women's Hospital, Harvard Medical School) ;
  • Hatfield, Dolph L. (Mouse Cancer Genetics Program, National Cancer Institute, National Institutes of Health) ;
  • Lee, Byeong Jae (School of Biological Sciences, Seoul National University)
  • Received : 2015.01.12
  • Accepted : 2015.01.09
  • Published : 2015.05.31
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Abstract

The 15-kDa selenoprotein (Sep15) is a selenoprotein residing in the lumen of the endoplasmic reticulum (ER) and implicated in quality control of protein folding. Herein, we established an inducible RNAi cell line that targets Sep15 mRNA in Chang liver cells. RNAi-induced Sep15 deficiency led to inhibition of cell proliferation, whereas cell growth was resumed after removal of the knockdown inducer. Sep15-deficient cells were arrested at the G1 phase by upregulating p21 and p27, and these cells were also characterized by ER stress. In addition, Sep15 deficiency led to the relocation of focal adhesions to the periphery of the cell basement and to the decrease of the migratory and invasive ability. All these changes were reversible depending on Sep15 status. Rescuing the knockdown state by expressing a silent mutant Sep15 mRNA that is resistant to siRNA also reversed the phenotypic changes. Our results suggest that SEP15 plays important roles in the regulation of the G1 phase during the cell cycle as well as in cell motility in Chang liver cells, and that this selenoprotein offers a novel functional link between the cell cycle and cell motility.

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References

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