Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Overexpression of Long Non-Coding RNA HOTAIR Promotes Tumor Growth and Metastasis in Human Osteosarcoma

  • Wang, Bo (Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University) ;
  • Su, Yun (Department of Orthopeadics, Affiliated Zhongshan Hospital of Dalian University) ;
  • Yang, Qun (Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University) ;
  • Lv, Decheng (Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University) ;
  • Zhang, Weiguo (Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University) ;
  • Tang, Kai (Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University) ;
  • Wang, Hong (Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University) ;
  • Zhang, Rui (Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University) ;
  • Liu, Yang (Department of Spine Surgery, First Affiliated Hospital of Dalian Medical University)
  • Received : 2014.11.21
  • Accepted : 2015.01.26
  • Published : 2015.05.31
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Abstract

Human osteosarcoma usually presented a high tendency to metastatic spread and caused poor outcomes, however, the underlying mechanism was still largely unknown. In the present study, using a series of in vitro experiments and an animal model, we investigated the roles of HOX antisense intergenic RNA (HOTAIR) during the proliferation and invasion of osteosarcoma. According with our results, HOTAIR was commonly overexpressed in osteosarcoma, which significantly correlated with advanced tumor stage, highly histological grade and poor prognosis. In vitro and in vivo experiments demonstrated that knockdown of HOTAIR could notably suppress cellular proliferation, inhibit invasion and decrease the secretion of MMP2 and MMP9 in osteosarcoma. Collectively, our results suggested that HOTAIR might be a potent therapeutic target for osteosarcoma.

Keywords

References

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