Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Preservation Conditions of Aqueous Samples Containing silver Nanomaterials

은나노물질을 포함한 수질시료의 보관조건

  • Kang, Mun Hee (Department of Geology and Research Institute of Natural Science, Gyeongsang National University) ;
  • Park, Sol (Department of Geology and Research Institute of Natural Science, Gyeongsang National University) ;
  • Lee, Sang-Woo (Department of Geology and Research Institute of Natural Science, Gyeongsang National University) ;
  • Kim, Hyun-A (School of Environmental Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Lee, Byung-Tae (School of Environmental Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Eom, Ig-Chun (Division of Risk Assessment, Department of Environmental Health Research, National Institute of Environmental Research) ;
  • Kim, Soon-Oh (Department of Geology and Research Institute of Natural Science, Gyeongsang National University)
  • 강문희 (경상대학교 지질과학과 및 기초과학연구소) ;
  • 박솔 (경상대학교 지질과학과 및 기초과학연구소) ;
  • 이상우 (경상대학교 지질과학과 및 기초과학연구소) ;
  • 김현아 (광주과학기술원 환경공학부) ;
  • 이병태 (광주과학기술원 환경공학부) ;
  • 엄익춘 (국립환경과학원 환경건강부 위해성평가연구과) ;
  • 김순오 (경상대학교 지질과학과 및 기초과학연구소)
  • Received : 2015.03.11
  • Accepted : 2015.04.23
  • Published : 2015.04.30
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Abstract

A prerequisite for precise quantification of nanomaterials contained in environmental samples is to prepare suitable preservation conditions of samples. This study was initiated to suggest preservation conditions of aqueous samples for analyses of metal nanomaterials. Variation in the size of silver nanomaterial (cit-AgNP) was observed according to change in various conditions, such as pH, electrolyte concentration, temperature, nanomaterial concentration, and time. Aggregation of AgNP was characterized for each environmental condition, and finally proper preservation conditions of samples were proposed based on experimental results on AgNP aggregation. In addition, the preservation period of sample was computed by the doublet time of AgNP. The results indicate that the aggregation rate of cit-AgNP was close to 0 at the conditions of pH of ${\geq}7$, electrolyte ($Ca(NO_3)_2$) concentration of ${\leq}3mM$, temperature of $4^{\circ}C$, and cit-AgNP concentration of ${\leq}2mg/L$. Furthermore, the experimental results on doublet time of cit-AgNP suggest that maximum preservation period was evaluated to be 15.79~17.53 days when the concentration of 100 nm cit-AgNP is assumed to be $1{\mu}g/L$ which is considered as an environmentally-relevant concentration of engineered nanomaterials. Our results suggest that samples should be preserved at $4^{\circ}C$ and analyzed within 2 weeks.

나노기술의 이용도가 높아지면서 나노물질 유출로 인한 환경오염 문제가 제기되고 있다. 수질시료 내 나노물질의 분석을 위해서는 시료의 교란을 최소화할 수 있는 보관조건 마련이 선결 요건이지만, 아직까지 적합한 보관조건이 제시되고 있지 않다. 이에 본 연구는 citrate로 코팅된 은 나노물질(cit-AgNP)을 대상으로 금속나노물질을 함유한 수질시료의 보관조건을 제시하고자 수행되었다. 이를 위해 시간분해 동적산란법(time-resolved dynamic light scattering)을 이용하여 pH, 배경용액의 농도, 온도, 나노물질의 농도 등과 같은 환경적인 조건과 시간에 따른 cit-AgNP의 크기 변화를 관찰하였다. 실험을 통한 각 환경조건별 AgNP의 응집특성을 해석하고 이러한 결과를 바탕으로 시료의 보관조건을 제시하였다. 그리고 AgNP의 입자농도와 응집속도의 선형적 관계로부터 구한 doublet time을 이용하여 시료의 보관기간을 산정하였다. 실험결과, pH는 7 이상, 배경 용액($Ca(NO_3)_2$)의 농도는 3 mM 이하, 온도는 냉장($4^{\circ}C$) 상태, 그리고 cit-AgNP의 농도는 2 mg/L 이하에서 응집속도가 0에 가까운 값을 나타내었다. 또한 수질시료 내 존재하는 100 nm cit-AgNP의 농도를 환경에 존재할 수 있는 낮은 수준인 $1{\mu}g/L$로 가정한 후 doublet time를 구한 결과, 가능한 시료의 보관기간은 15.79~17.53일 정도인 것으로 조사되었다. 하지만 pH와 배경 용액의 농도 조절은 시료의 변질과 교란이 우려되기 때문에 보관조건으로 일반화하여 제시하는 것은 적절하지 않고, 나노물질 자체의 농도를 조절하는 것은 수질시료 내 나노물질의 농도 등의 분석을 위한 시료의 보관조건으로 바람직하지 않다. 그러므로 본 연구의 결과로부터 일반화하여 제시할 수 있는 보관조건은 냉장($4^{\circ}C$) 상태에서 2주일 정도인 것으로 판단된다.

Keywords

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