Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Production of biomass and bioactive compounds from adventitious root cultures of Polygonum multiflorum using air-lift bioreactors

생물반응기를 이용한 적하수오 부정근의 바이오매스와 생리활성물질 대량생산

  • Lee, Kyung-Ju (Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University) ;
  • Park, Youngki (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kim, Ja-Young (Saimdang Cosmetics Co., Ltd.) ;
  • Jeong, Taek-Kyu (Saimdang Cosmetics Co., Ltd.) ;
  • Yun, Kyung-Seop (Saimdang Cosmetics Co., Ltd.) ;
  • Paek, Kee-Yoeup (Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University) ;
  • Park, So-Young (Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University)
  • Received : 2015.03.09
  • Accepted : 2015.03.23
  • Published : 2015.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the productivity of biomass and antioxidant compounds in Polygonum multiflorum by culturing explants in air-lift bioreactor containing Murashige and Skoog (MS) medium, by adding different concentrations of auxins [indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)], sucrose, methyl jasmonate (MeJA), and salicylic acid (SA). Results of this study revealed that the explants culturing on the medium supplemented with $9.84{\mu}M$ IBA and 50 g/L sucrose were observed to have higher productivity of biomass and bioactive compound than other treatments used. Thus, we expect that these results will be helpful for large-scale production of biomass and antioxidant compounds from Polygonum multiflorum.

본 연구는 약용식물인 적하수오의 부정근과 생리활성물질 대량생산을 위해 생물반응기 배양 조건을 확립하고자 실시되었다. 이를 위하여 생물반응기 배양 시 배지 내 무기물 함량과, auxin의 종류와 농도, sucrose 농도가 적하수오 부정근의 생장과 총 phenolics와 flavonoids 함량에 미치는 영향을 조사하였으며, 배양 중 methyl jasmonate (MeJA)와 salicylic acid (SA)의 첨가가 생리활성물질 축적에 미치는 영향을 조사하였다. 그 결과 $9.84{\mu}M$ IBA와 50 g/L sucrose가 첨가된 1배 MS배지에서 최적의 부정근 생장이 이루어졌으며 생리활성물질 축적도 가장 높았다. MeJA와 SA 처리시 적하수오 부정근의 생장과 생리활성물질 축적은 오히려 감소하였다. 본 연구 결과는 산업적 목적을 위한 적하수오의 부정근과 생리활성물질 대량생산시 기초자료로 활용될 수 있을 것으로 기대된다.

Keywords

References

  1. Abbasi BH, Liu E, Saxena PK, Liu CZ (2009) Cichoric acid production from hairy root cultures of Echinacea purpurea grown in a modified airlift bioreactor. J Chem Technol Biot 84 : 1697-1701 https://doi.org/10.1002/jctb.2233
  2. Ali MB, Yu KW, Hahn EJ, Paek KY (2006a) Antioxidantive responses of Echinacea angustifolia cultured roots to different levels of $CO_2$ in bioreactor liquid cultures. Enz Microb Tech 25 : 1122-1132
  3. Ali MB, Yu KW, Hahn EJ, Paek KY (2006b) Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and non-enzymatic antioxidant in suspention culture Panax ginseng roots in bioreactors. Plant Cell Rep 25 : 613-620 https://doi.org/10.1007/s00299-005-0065-6
  4. Amiarouche L, Stuchbury T, Mattews S (1985) Comparisons of cultivar performance on different nutrient media in a routine method for potato micropropagation. Potato Res 28 : 469-478 https://doi.org/10.1007/BF02357525
  5. Chakrabarty D, Hahn EJ, Yoon YJ, Paek KY (2003) Micropropagation of apple rootstock M.9 EMLA using bioreactor. J Hortic Sci Biotech 78 : 605-609 https://doi.org/10.1080/14620316.2003.11511671
  6. Cheruvathur MK, Thomas TD (2014) Effects of plant growth regulators and elicitors on rhinacanthin accumulation in hairy root cultures of Rhinacanthus nasutus (L.) Kurz. Plant Cell Tiss Org 118 : 169-177 https://doi.org/10.1007/s11240-014-0473-9
  7. Choi JW, Lee HS, Kim YG, Kim BM, Kim IH, Lee CH (2012) Effect of Polygonum multiflorum Thunberg Extract on Lipid Metabolism in Rats Fed High-Cholesterol Diet. J Korean Soc Food Sci Nutr 41 : 957-962 https://doi.org/10.3746/jkfn.2012.41.7.957
  8. Coste A, Vlase L, Halmagyi A, Deliu C, Coldea G (2011) Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell Tiss Org 106 : 279-288 https://doi.org/10.1007/s11240-011-9919-5
  9. Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresource Technol 101 : 4708-4716 https://doi.org/10.1016/j.biortech.2010.01.115
  10. Cui XH, Murthy HN, Jin YX, Yim YH, Kim JY, Paek KY (2011) Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor. Bioresource Technol 102 : 10072-10079 https://doi.org/10.1016/j.biortech.2011.08.044
  11. Cui HY, Baque MA, Lee EJ, Paek KY (2013) Scale-up of adventitious root cultures of Echinacea angustifolia in a pilot-scale bioreactor for the production of biomass and caffeic acid derivatives. Plant Biotechnol Rep 7 : 297-308 https://doi.org/10.1007/s11816-012-0263-y
  12. Ebel J, Mithofer A (1998) Early events in the elicitation of plant defense. Planta 206 : 335-348 https://doi.org/10.1007/s004250050409
  13. George EF, Puttock DJM, Geroge HJ (1988) Plant culture media: Commentary and analysis. Vol. 2. Exegetics Ltd, Edington, UK
  14. Hasan NA, Hussein S, Ibrahim R (2014) Plant growth regulator effect on adventitious roots induction of Labisia pumila. Malaysian J Fundamental Appl Sci 10 : 49-52
  15. Izbianska K, Jelonek MA, Deckert J (2014) Phenylpropanoid pathway metabolites promote tolerance response of lupine roots to lead stress. Ecotox Environl Safe 110 : 61-67 https://doi.org/10.1016/j.ecoenv.2014.08.014
  16. Jeong CS, Murthy HN, Hahn EJ, Paek KY (2009) Inoculum size and auxin concentration influence the growth of adventitious roots and accumulation of ginsenosides in suspension cultures of ginseng (Panax ginseng C.A. Meyer). Acta Physiol Plant 31 : 219-222 https://doi.org/10.1007/s11738-008-0206-y
  17. Lee EJ, Mobin M, Hahn EJ, Paek KY (2006) Effects of sucrose, inoculum density, auxins, and aeration volume on cell growth of Gymnema sylvestre. J Plant Biol 49 : 427-431 https://doi.org/10.1007/BF03031122
  18. Lee EJ, Kim MK, Paek KY (2010) Auxin and cytokinin affect biomass and bioactive compound production from adventitious roots of Eleutherococcus koreanum. Kor J Hort Sci Technol 28 : 678-684
  19. Lin HT, Nah SL, Huang YY, Wu SC (2010) Potential antioxidant components and characteristics of fresh Polygonum multiflorum. J Food Drug Anal 18 : 120-127
  20. Murthy HN, Hahn EJ, Paek KY (2008) Adventitious roots and secondary metabolism. Chinese J Biotechnol 24 : 711-716 https://doi.org/10.1016/S1872-2075(08)60035-7
  21. Murthy HN, Kim YS, Park SY, Paek KY (2014) Biotechnological production of caffeic acid derivatives from cell and organ cultures of Echinacea species. Appl Microbiol Biot 14 : 5962-5966
  22. Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotech Adv 20 : 101-153 https://doi.org/10.1016/S0734-9750(02)00007-1
  23. Sakanaka S, Tachibana Y, Okada Y (2005) Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinohacha). Food Chem 89 : 569-575 https://doi.org/10.1016/j.foodchem.2004.03.013
  24. Sivakumar G, Yu KW, Paek KY (2005) Production of biomass and ginsenosides from adventitious roots of Panax ginseng in bioreactor cultures. Eng Life Sci 5 : 333-342 https://doi.org/10.1002/elsc.200520085
  25. Sivakumar G, Yu KW, Paek KY (2006) Enhanced production of bioactive ginsenosides from adventitious roots of Panax ginseng in bioreactor culture. J Hortic Sci Biotech 81 : 549-552 https://doi.org/10.1080/14620316.2006.11512102
  26. Southwell LA. and Bourke CA (2001) Seasonal variation in hypercin content of Hypericum perforatum L. (St. John's wort). Phytochemistry 56 : 437-441 https://doi.org/10.1016/S0031-9422(00)00411-8
  27. Verpoorte R, Contin A, Memelink J (2002) Biotechnology for the production of plant secondary metabolites. Phytochem Rev 1 : 13-25 https://doi.org/10.1023/A:1015871916833
  28. Wu CH, Dewir YH, Hahn EJ, Paek KY (2006) Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol 49 : 193-199 https://doi.org/10.1007/BF03030532
  29. Yaseen M, Ahmad T, Sablok G, Standardi A, Hafiz IA (2013) Review: rol of carbon sources for in vitro plant growth and development. Mol Biol Rep 40 : 2837-2849 https://doi.org/10.1007/s11033-012-2299-z
  30. Yu KW, Hahn EJ, Paek KY (2000) Production of adventitious ginseng roots using bioreactors. Korean J Plant Tiss Cult 27 : 309-315
  31. Zhao J, LC Davis, R Verpoorte (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23 : 283-333 https://doi.org/10.1016/j.biotechadv.2005.01.003

Cited by

  1. Kinsenoside and polysaccharide production by rhizome culture of Anoectochilus roxburghii in continuous immersion bioreactor systems 2017, https://doi.org/10.1007/s11240-017-1302-8
  2. Adventitious root culture of Polygonum multiflorum for phenolic compounds and its pilot-scale production in 500 L-tank vol.130, pp.1, 2017, https://doi.org/10.1007/s11240-017-1212-9
  3. Rapid comparison of metabolic equivalence of standard medicinal parts from medicinal plants and their in vitro-generated adventitious roots using FT-IR spectroscopy vol.42, pp.3, 2015, https://doi.org/10.5010/JPB.2015.42.3.257
  4. Secondary metabolism of pharmaceuticals in the plant in vitro cultures: strategies, approaches, and limitations to achieving higher yield vol.132, pp.2, 2018, https://doi.org/10.1007/s11240-017-1332-2