Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Antioxidant Capacity and Protective Effects on Neuronal PC-12 Cells of Domestic Bred Kiwifruit

국내 육성 참다래의 항산화능 및 PC-12 신경세포 보호 효과

  • Lee, Inil (Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Bong Han (Graduate School of Biotechnology, Kyung Hee University) ;
  • Eom, Seok Hyun (Skin Biotechnology Center, Kyung Hee University) ;
  • Oh, Chang-Sik (Department of Horticultural Biotechnology, Kyung Hee University) ;
  • Kang, Hee (Department of East-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Cho, Youn-Sup (Fruit Research Institute, Jeollanam-do Agricultural Research and Extension Services) ;
  • Kim, Dae-Ok (Graduate School of Biotechnology, Kyung Hee University)
  • 이인일 (경희대학교 생명공학원) ;
  • 이봉한 (경희대학교 생명공학원) ;
  • 엄석현 (경희대학교 피부생명공학센터) ;
  • 오창식 (경희대학교 원예생명공학과) ;
  • 강희 (경희대학교 동서의과학과) ;
  • 조윤섭 (전라남도 농업기술원 과수연구소) ;
  • 김대옥 (경희대학교 생명공학원)
  • Received : 2014.07.23
  • Accepted : 2014.12.03
  • Published : 2015.04.30
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Abstract

This study was conducted to comparatively evaluate antioxidant capacity (AC) of seven cultivars of kiwifruit (Actinidia spp.) and their protective effects on neuronal PC-12 cells. The contents of total phenolics (TP) and total flavonoids (TF) of kiwifruits were also examined. Five cultivars of kiwifruit, Actinidia chinensis (cv. Haehyang and cv. Haegeum), A. eriantha (cv. Bidan), A. arguta ${\times}$ A. deliciosa (cv. Mansoo), and A. arguta (cv. Chiak), were bred in Korea, while two cultivars, A. deliciosa (cv. Hayward) and A. linguiensis (accession number 041AE), originated from New Zealand and China, respectively. Skin extracts of kiwifruit showed higher TP, TF, and AC than flesh extracts. The highest levels of TP and AC were found in cv. Bidan flesh extract among cultivars studied, but the TF content of cv. Bidan flesh extract was the lowest. The kiwifruit bred in Korea had higher AC than their counterparts. AC of kiwifruit had a highly positive linear correlation with TP and TF. The flesh extracts from cv. Hayward, cv. Haehyang, and cv. Haegeum significantly (p < 0.05) prevented PC-12 cells from oxidative stress induced using $H_2O_2$ compared to a control with $H_2O_2$ only. Overall, our results suggest that kiwifruit bred in Korea may offer a good source of antioxidants and serve as functional materials.

본 연구는 참다래의 항산화능을 측정하고, 참다래 과육 추출물의 PC-12 세포주에 대한 보호 효과를 비교 평가하였다. 또한, 참다래의 총페놀 함량 및 총플라보노이드 함량를 측정하였다. 국내에서 육성 재배 중인 참다래인 해향(Actinidia chinensis cv. Haehyang), 해금(A. chinensis cv. Haegeum), 비단(A. eriantha cv. Bidan), 만수(A. arguta ${\times}$ A. deliciosa cv. Mansoo), 치악(A. arguta cv. Chiak) 등 5품종과 뉴질랜드 유래 헤이워드(A. deliciosa cv. Hayward), 중국 유래 041AE(A. linguiensis accession number 041AE) 포함 총 7품종을 시험에 사용하였다. 참다래의 껍질 추출물은 총페놀함량, 총플라보노이드 함량, 항산화능이 과육 추출물보다 더 높았다. 7품종 중에서 비단 품종 과육이 총페놀 함량과 항산화능이 가장 높았지만, 총플라보노이드 함량은 가장 낮았다. 참다래 추출물의 항산화능은 총페놀 함량과 총플라보노이드 함량과 양의 선형적 상관관계를 가졌다. 해향, 해금, 헤이워드 품종은 세포 내의 산화적 스트레스를 감소시켜 PC-12 세포를 유의적으로 보호하였다. 본 연구는 해금, 해향, 비단 등 국내 개발 육성 참다래가 외래 도입종에 비해 더 높은 항산화능을 갖는다는 결과를 보였다. 이는 국내 육성 참다래가 높은 생리활성물질을 제공하는 기능성 소재로 활용 가능하다는 것을 시사한다.

Keywords

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