Transactions of the Korean hydrogen and new energy society (한국수소및신에너지학회논문집)

The Korean Hydrogen and New Energy Society (한국수소및신에너지학회)
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A Study on Glucose Sensing Measured by Catalyst Containing Multiple Layers of Glucose Oxidase and Gold Nano Rod

글루코스산화효소와 금나노로드 입자의 다층막으로 구성된 촉매를 이용하여 측정한 글루코스 센싱에 대한 연구

  • Chung, Yong-Jin (Graduate School of Energy and Environment, Seoul National University of Science and Technology) ;
  • Hyun, Kyuhwan (Graduate School of Energy and Environment, Seoul National University of Science and Technology) ;
  • Han, Sang Won (Department of Chemical and Biomolecular Engineering, Yonsei University) ;
  • Min, Ji Hong (Department of Chemical and Biomolecular Engineering, Yonsei University) ;
  • Chun, Seung-Kyu (Graduate School of Energy and Environment, Seoul National University of Science and Technology) ;
  • Koh, Won-Gun (Department of Chemical and Biomolecular Engineering, Yonsei University) ;
  • Kwon, Yongchai (Graduate School of Energy and Environment, Seoul National University of Science and Technology)
  • 정용진 (서울과학기술대학교 에너지환경대학원) ;
  • 현규환 (서울과학기술대학교 에너지환경대학원) ;
  • 한상원 (연세대학교 화공생명공학과) ;
  • 민지홍 (연세대학교 화공생명공학과) ;
  • 천승규 (서울과학기술대학교 에너지환경대학원) ;
  • 고원건 (연세대학교 화공생명공학과) ;
  • 권용재 (서울과학기술대학교 에너지환경대학원)
  • Received : 2015.04.07
  • Accepted : 2015.04.30
  • Published : 2015.04.30
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Abstract

In this study, we propose a catalyst structure including enzyme and metal nano rod for glucose sensing. In the catalyst structure, glucose oxidase (GOx) and gold nano rod (GNR) are alternatingly immobilized on the surface of carbon nanotube (CNT), while poly(ethyleneimine) (PEI) is inserted in between the GOx and GNR to fortify their bonding and give them opposite polarization ($[GOx/GNR]_nPEI/CNT$). To investigate the impact of $[GOx/GNR]_nPEI/CNT$ on glucose sensing, some electrochemical measurements are carried out. Initially, their optimal layer is determined by using cyclic voltammogram and as a result of that, it is proved that $[GOx/GNR/PEI]_2/CNT$ is the best layer. Its glucose sensitivity is $13.315{\mu}AmM^{-1}cm^{-2}$. When it comes to the redox reaction mechanism of flavin adenine dinucleotide (FAD) within $[GOx/GNR/PEI]_2/CNT$, (i) oxygen plays a mediator role in moving electrons and protons generated by glucose oxidation reaction to those for the reduction reaction of FAD and (ii) glucose does not affect the redox reaction of FAD. It is also recognized that the $[GOx/GNR/PEI]_3/CNT$ is limited to the surface reaction and the reaction is quasi-reversible.

Keywords

References

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