Journal of Applied Biological Chemistry

  • 제58권1호
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  • Pages.81-88
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  • 2015
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  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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Bacillus pumilus IJ-1의 생물계면활성제 생산을 위한 배양 특성

Characteristics of Culture Conditions for the Production of Biosurfactant by Bacillus pumilus IJ-1

  • Park, Eunjin (Department of Neuroscience, Graduate School, Inje University) ;
  • Kim, Jiyeon (College of General Education, Inje University)
  • 투고 : 2014.12.09
  • 심사 : 2015.01.29
  • 발행 : 2015.03.31
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초록

Bacillus pumilus IJ-1의 생물계면활성제 생산을 위한 배지 조성의 특성을 조사하기 위하여 다양한 탄소원, 질소원 및 무기염이 미치는 영향을 검토하였다. 그 결과 B. pumilus IJ-1은 0.5%(w/v) tryptone을 첨가하여 배양하였을 때 가장 많은 생물계면활성제를 생산하였다. B. pumilus IJ-1은 0-10% (w/v)의 NaCl 농도와 pH 5-10, $20-45^{\circ}C$의 환경에서 생장이 가능하였다. 그리고 생물계면활성제 생산은 pH 9.0의 초기 배지에서 $20^{\circ}C$, 72시간 동안 배양했을 때 최대였으며, 이러한 배양 조건 하에서 얻어진 배양액의 표면장력은 27.0 dyne/cm이었다.

To characterize the culture medium for the biosurfactant production by Bacillus pumilus IJ-1, the influences of various carbon, nitrogen and mineral sources were assessed. As a result, the highest biosurfactant production was observed after 96 h cultivation containing 0.5% (w/v) tryptone. The strain was able to grow and produce biosurfactant at 0-10% (w/v) NaCl, in the pH range of 5-10, and at $20-45^{\circ}C$. Optimal culture conditions for the biosurfactant production were at $20^{\circ}C$ and pH 9.0 after 72 h incubation and the surface tension of biosurfactant was 27.0 dyne/cm.

키워드

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피인용 문헌

  1. Optimization Using 33 Full-Factorial Design for Crude Biosurfactant Activity from Bacillus pumilus IJ-1 in Submerged Fermentation vol.48, pp.1, 2015, https://doi.org/10.4014/mbl.1908.08001