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Characteristics of Culture Conditions for the Production of Biosurfactant by Bacillus pumilus IJ-1

Bacillus pumilus IJ-1의 생물계면활성제 생산을 위한 배양 특성

  • Park, Eunjin (Department of Neuroscience, Graduate School, Inje University) ;
  • Kim, Jiyeon (College of General Education, Inje University)
  • Received : 2014.12.09
  • Accepted : 2015.01.29
  • Published : 2015.03.31

Abstract

To characterize the culture medium for the biosurfactant production by Bacillus pumilus IJ-1, the influences of various carbon, nitrogen and mineral sources were assessed. As a result, the highest biosurfactant production was observed after 96 h cultivation containing 0.5% (w/v) tryptone. The strain was able to grow and produce biosurfactant at 0-10% (w/v) NaCl, in the pH range of 5-10, and at $20-45^{\circ}C$. Optimal culture conditions for the biosurfactant production were at $20^{\circ}C$ and pH 9.0 after 72 h incubation and the surface tension of biosurfactant was 27.0 dyne/cm.

Bacillus pumilus IJ-1의 생물계면활성제 생산을 위한 배지 조성의 특성을 조사하기 위하여 다양한 탄소원, 질소원 및 무기염이 미치는 영향을 검토하였다. 그 결과 B. pumilus IJ-1은 0.5%(w/v) tryptone을 첨가하여 배양하였을 때 가장 많은 생물계면활성제를 생산하였다. B. pumilus IJ-1은 0-10% (w/v)의 NaCl 농도와 pH 5-10, $20-45^{\circ}C$의 환경에서 생장이 가능하였다. 그리고 생물계면활성제 생산은 pH 9.0의 초기 배지에서 $20^{\circ}C$, 72시간 동안 배양했을 때 최대였으며, 이러한 배양 조건 하에서 얻어진 배양액의 표면장력은 27.0 dyne/cm이었다.

Keywords

References

  1. Ahimou F, Jacques P, and Deleu M (2000) Surfactin and iturin A effects on Bacillus subtilis surface hydrophobicity. Enzyme Microb Technol 27, 749-54. https://doi.org/10.1016/S0141-0229(00)00295-7
  2. Banat IM, Makkar RS, and Cameotra SS (2000) Potential commercial applications of microbial surfactants. Appl Microbiol Biotechnol 53, 495-508. https://doi.org/10.1007/s002530051648
  3. Bognolo G (1999) Biosurfactants as emulsifying agents for hydrocarbons. Colloids Surf A Physicochem Eng Asp 152, 41-52. https://doi.org/10.1016/S0927-7757(98)00684-0
  4. Deshpande S, Shiau BJ, Wade D, Sabatini DA, and Harwell JH (1999) Surfactant selection for enhancing ex situ soil washing. Water Res 33, 351-60. https://doi.org/10.1016/S0043-1354(98)00234-6
  5. Dibble JT and Bartha R (1979) Effect of environmental parameters on the biodegradation of oil sludge. Appl Environ Microbiol 37, 729-39.
  6. Fonseca RR, Silva AJ, De Franca FP, Cardoso VL, and Servulo EF (2007) Optimizing carbon/nitrogen ratio for biosurfactant production by a Bacillus subtilis strain. Appl Biochem Biotechnol 137-140, 471-86. https://doi.org/10.1007/s12010-007-9073-z
  7. Ghojavand H, Vahabzadeh F, Roayaei E, and Shahraki AK (2008) Production and properties of a biosurfactant obtained from a member of the Bacillus subtilis group (PTCC 1696). J Colloid Interface Sci 324, 172-6. https://doi.org/10.1016/j.jcis.2008.05.001
  8. Gogotov IN and Miroshnikov AI (2009) The influence of growth medium composition and physicochemical factors on biosurfactant production by the bacterium Bacillus licheniformis VKM B-511. Prikl Biokhim Mikrobiol 45, 654-8.
  9. Greek BF (1991) Sales of detergents growing desoite recession. Chem Eng News 69, 25-52.
  10. Haddad NI, Wang J, and Mu B (2009) Identification of a biosurfactant producing strain: Bacillus subtilis HOB2. Protein Pept Lett 16, 7-13. https://doi.org/10.2174/092986609787049358
  11. Hassanshahian M, Emtiazi G, and Cappello S (2012) Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea. Mar Pollut Bull 64, 712. https://doi.org/10.1016/j.marpolbul.2012.01.042
  12. Hur SH, Yang JS, and Hong JH (2002) Production of biosurfactant using Bacillus spp.. J Korean Soc Food Sci Nutr 31, 389-93. https://doi.org/10.3746/jkfn.2002.31.3.389
  13. Joshi S, Bharucha C, and Desai AJ (2008) Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B. Bioresour Technol 99, 4603-8. https://doi.org/10.1016/j.biortech.2007.07.030
  14. Kim HS, Yoon BD, Lee CH, Suh HH, Oh HM, Katsuragi T et al. (1997) Production and properties of a lipopeptide biosurfactant from Bacillus subtilis C9. J Ferment Bioeng 84, 41-6. https://doi.org/10.1016/S0922-338X(97)82784-5
  15. Kim JS, Song HS, Chung NH, and Bang WG (2005) Optimization of production conditions of biosurfactant from Bacillus sp. and its purification. J Korean Soc Appl Biol Chem 48, 109-14.
  16. Kim JY (2014) Isolation and characterization of a biosurfactant-producing bacterium Bacillus pumilus IJ-1 from contaminated crude oil collected in Taean, Korea. J Korean Soc Appl Biol Chem 57, 514.
  17. Lang S (2002) Biological amphiphiles (microbial biosurfactants). Curr Opin Colloid Interface Sci 7, 12-20. https://doi.org/10.1016/S1359-0294(02)00007-9
  18. Makkar RS and Cameotra SS (1998) Production of biosurfactant at mesophilic and thermophilic conditions by a strain of Bacillus subtilis. J Ind Microbiol Biotechnol 20, 48-52. https://doi.org/10.1038/sj.jim.2900474
  19. Mukherjee S, Das P, and Sen R (2006) Towards commercial production of microbial surfactants. Trends Biotechnol 24, 509-15. https://doi.org/10.1016/j.tibtech.2006.09.005
  20. Mulligan CN (2005) Environmental applications for biosurfactants. Environ Pollut 133, 183-98. https://doi.org/10.1016/j.envpol.2004.06.009
  21. Najkar D and Gutnick DL (2003) Involvement of a protein tyrosine kinase in production of the polymeric bioemulsifier emulsan from the oildegrading strain Acinetobacter lwoffii RAG-1. J Bacteriol 185, 1001-9. https://doi.org/10.1128/JB.185.3.1001-1009.2003
  22. Pagilla KR, Sood A, and Kim H (2002) Gordonia (nocardia) amarae foaming due to biosurfactant production. Water Sci Technol 46, 519-24.
  23. Pirog TP, Shevchuk TA, Voloshina IN, and Karpenko EV (2004) Production of surfactants by Rhodococcus erythropolis strain EK-1, grown on hydrophilic and hydrophobic substrates. Appl Biochem Microbiol 40, 470-5. https://doi.org/10.1023/B:ABIM.0000040670.33787.5f
  24. Sahoo S, Datta S, Biswas D, and Banik Choudhury R (2010) Biosurfactant production from n-paraffins by an air isolate Pseudomonas aeruginosa OCD1. J Oleo Sci 59, 601-5. https://doi.org/10.5650/jos.59.601
  25. Shim SH and Park KR (2006) Characteristics of biosurfactant producing Pseudomonas sp. G314. Kor J Mocrobiol 42, 286-93.
  26. Thavasi R, Jayalakshmi S, Balasubramanian T, and Banat IM (2007) Biosurfactant production by Corynebacterium kutscheri from waste motor lubricant oil and peanut oil cake. Lett Appl Microbiol 45, 686-91. https://doi.org/10.1111/j.1472-765X.2007.02256.x
  27. Walker JD and Colwell RR (1975) Some effect of petroleum on estuarine and marine microorganisms. Can J Microbiol 21, 305-13. https://doi.org/10.1139/m75-044
  28. Wei YH, Wang LF, Changy JS, and Kung SS (2003) Identification of induced acidification in iron-enriched cultures of Bacillus subtilis during biosurfactant fermentation. J Biosci Bioeng 96, 174-8. https://doi.org/10.1016/S1389-1723(03)90121-6

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