대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제41권1호
  • /
  • Pages.1-7
  • /
  • 2015
  • /
  • 1226-2587(pISSN)
  • /
  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
권호 표지
DOI QR코드

DOI QR Code

자외선에 의한 세포손상에 대한 말채나무잎의 보호효과

The Protective Effects of Cornus walteri Wanger Leaves against UV Induced Cellular Damage in Human Fibroblast

  • 박현철 ((주)사임당화장품 기술연구소) ;
  • 정택규 ((주)사임당화장품 기술연구소) ;
  • 윤경섭 ((주)사임당화장품 기술연구소)
  • 투고 : 2014.10.13
  • 심사 : 2015.01.05
  • 발행 : 2015.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

말채나무는 한국의 민간요법으로 사용되던 약재이다. 자외선은 피부의 광손상을 일으키는 것으로 알려져 있다. 본 연구에서는 자외선에 의한 손상된 세포를 회복시키기 위해서 효소처리 된 말채나무잎추출물(CWE)을 사용하였다. 섬유아세포에 UVB를 조사한 후, CWE를 처리하여 세포의 회복을 조사하였다. UVB를 조사한 섬유아세포에는 caspase-3 활성, phospho-p53, ${\gamma}H2AX$, cyclobutane pyrimidine dimers (CPDs) formation, 그리고 DNA fragmentation이 증가하게 된다. 그러나 CWE를 UVB가 조사된 섬유아세포에 12 h 처리하였을 때 caspase-3 활성, phospho-p53, ${\gamma}H2AX$, CPDs formation, 그리고 DNA fragmentation이 감소하였다. 또한 CWE은 인체첩포시험을 통해 인체피부에 자극을 유발하지 않음을 확인하였다. 이러한 결과를 종합할 때 CWE는 자외선에 대한 광보호 효과가 있는 원료로서 가능성을 가지고 있다고 판단된다.

Cornus walteri Wanger has been used in folk medicine in Korea. Ultraviolet (UV) irradiation has been known as a major cause of photo damage in skin. In the present study, research on how to cure damaged cells by UVB was conducted using an extract of Cornus walteri Wanger leaves (CWE), which was treated with an enzyme. CWE was applied to human dermal fibroblasts (HDFs) affected by UVB. UVB-irradiated HS68 cells showed increased caspase-3 activity, phosphorylation of p53, ${\gamma}H2AX$, cyclobutane pyrimidine dimers (CPDs) formation, and DNA fragmentation compared with non-irradiated cells. However, all these effects were inhibited by treatment with CWE for 12 h after UVB irradiation. Furthermore, CWE has proved not to cause primary skin irritation through the human patch test. Collectively, these results suggest that CWE could be a new potential candidate as photoprotective agent against UVB-induced cellular damage in HDFs.

키워드

참고문헌

  1. H. Matz, UV light and its interaction with cutaneous receptors, Dermatol. Clin., 25, 633 (2007). https://doi.org/10.1016/j.det.2007.06.006
  2. H. L. Lo, S. Nakajima, L. Ma, B. Walter, A. Yasui, D. W. Ethell, and L. B. Owen, Differential biologic effects of CPD and 6-4PP UV-induced DNA damage on the induction of apoptosis and cell-cycle arrest, BMC Cancer, 5, 135 (2005). https://doi.org/10.1186/1471-2407-5-135
  3. S. Elmore, Apoptosis: a review of programmed cell death, Toxicol. Pathol., 35(4), 495 (2007). https://doi.org/10.1080/01926230701320337
  4. S. Haupt, M. Berger, Z. Goldberg, and Y. Haupt, Apoptosis - the p53 network, J. Cell Science, 116, 4077 (2003). https://doi.org/10.1242/jcs.00739
  5. A. Svobodova, J. Psotova, and D. Walterova, Natural phenolics in the prevention of UV-induced skin damage, A review. Biomed. Papers, 147(2), 137 (2003). https://doi.org/10.5507/bp.2003.019
  6. C. B. Lee, Coloured Flora of Korea, 594, Hyangmunsa, Seoul (1989).
  7. W. G. Lee, Coloured standard illustrations of korean plants, 249, Dosuh Press, Seoul (1996).
  8. W. H. Choi, W. Y. Park, B. Y. Hwang, G. J. Oh, S. J. Kang, K. S. Lee, and J. S. Ro, Phenolic compounds from the stem bark of Cornus walteri Wnager, Kor. J. Pharmacogn., 29, 217 (1998).
  9. C. S. Lim, C. Y. Li, Y. M. Kim, W. Y. Lee, and H. I. Rhee, The inhibitory effect of Cornus walteri extract against ${\alpha}$-amylase, J. Korean Soc. Appl. Biol. Chem., 48, 103 (2005).
  10. W. H. Park and Y. Y. Cha, Effect of stem bark extracts of Cornus walteri Wanger on the lipid lowering, anti-oxidative activity and concentration of proinflammatory cytokines in rat fed high fat diet, J. Oriental Rehab. Med., 19, 59 (2009).
  11. P. J. Frosch and A. M. Kligman, The soap chamber test, A new method for assessing the irritancy of soaps, J. Am. Acad. Dermatol., 1(1), 35 (1979).
  12. CTFA Safety Testing Guideline: The Cosmetic, 20005, Toiletry and Fragrance Association, Inc. Washington, D. C. (1981).
  13. CTFA Safety Testing Guideline: The Cosmetic, 20036 Toiletry and Fragrance Association, Inc. Washington, D. C. (1991).
  14. L. E. Broker, A. E. Kruyt, and G. Giaccone, Cell death independent of caspases: a review, Clin. Cancer Res., 11, 3155 (2005). https://doi.org/10.1158/1078-0432.CCR-04-2223
  15. H. Xu, Y. Yan, L. Li, S. Peng, T. Qu, and B. Wang, Ultraviolet B-induced apoptosis of human skin fibroblasts involves activation of caspase-8 and -3 with increased expression of vimentin, Photodermatol. Photoimmunology. Photomed., 26(4), 198 (2010). https://doi.org/10.1111/j.1600-0781.2010.00522.x
  16. E. P. Rogakou, D. R. Pilch, A. H. Orr, V. S. Ivanova, and W. M. Bonner, DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139, J. Biol. Chem., 273, 5858 (1998). https://doi.org/10.1074/jbc.273.10.5858
  17. L. Barnes, M. Dumas, M. Juan, E. Noblesse, A. Tesniere, S. Schnebert, B. Guillot, and J. P. Moles, Gamma H2AX, an accurate marker that analyzes UV genotoxic effects on human keratinocytes and on human skin, Photochem. Photobiol., 86(4), 933 (2010). https://doi.org/10.1111/j.1751-1097.2010.00744.x
  18. C. Lu, F. Zhu, Y. Y. Cho, F. Tang, T. Zykova, W. Y. Ma, A. M. Bode, and Z. Dong, Cell apoptosis: requirement of H2AX in DNA ladder formation, but not for the activation of caspase-3, Mol. Cell, 23(1), 121 (2006). https://doi.org/10.1016/j.molcel.2006.05.023
  19. E. P. Rogakou, W. Nieves-Neira, C. Boon, Y. Pommier, and W. M. Bonner, Initiation of DNA fragmentation during apoptosis induces phosphorylation of H2AX histone at serine 139, J. Biol. Chem., 275, 9390 (2000). https://doi.org/10.1074/jbc.275.13.9390
  20. L. Latonen, Y. Taya, and M. Laiho, UV-radiation induces dose- dependent regulation of p53 response and modulates p53-HDM2 interaction in human fibroblasts, Oncogene, 20(46), 6784 (2001). https://doi.org/10.1038/sj.onc.1204883