Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Genetic relationship analysis of Pleurotus pulmonarius strains using RAPD

RAPD를 이용한 산느타리(Pleurotus pulmonarius) 수집균주 유연관계 분석

  • Lee, Jae-Hong (Agricultural Environment Research Section, Gangwon Province Agriculture Research and Extension Services) ;
  • Lee, Nam-Gil (Agricultural Environment Research Section, Gangwon Province Agriculture Research and Extension Services) ;
  • Mun, Youn-Gi (Agricultural Environment Research Section, Gangwon Province Agriculture Research and Extension Services) ;
  • Jeong, Tae-Sung (Agricultural Environment Research Section, Gangwon Province Agriculture Research and Extension Services) ;
  • Kwon, Sun-Bae (Agricultural Environment Research Section, Gangwon Province Agriculture Research and Extension Services) ;
  • Kim, Jae-Rok (Agricultural Environment Research Section, Gangwon Province Agriculture Research and Extension Services) ;
  • Kim, Jin-Won (Department of Environmental horticulture, University of Seoul)
  • 이재홍 (강원도농업기술원 환경농업연구과) ;
  • 이남길 (강원도농업기술원 환경농업연구과) ;
  • 문윤기 (강원도농업기술원 환경농업연구과) ;
  • 정태성 (강원도농업기술원 환경농업연구과) ;
  • 권순배 (강원도농업기술원 환경농업연구과) ;
  • 김재록 (강원도농업기술원 환경농업연구과) ;
  • 김진원 (서울시립대학교 환경원예학과)
  • Received : 2015.02.16
  • Accepted : 2015.03.30
  • Published : 2015.03.31
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Abstract

Genetic relationship analysis by PCR reactions with 25 primers could produce relevant classifications of the 21 P. pulmonarius (Fr) Quel. isolates collected from 2005. They were classified by two groups with similarity coefficient 0.37. All of the P. pulmonarius cultivars belonged to group 2. New cultivar 'Hwasan' presented high similarity coefficient to parental strains, and that of 'Hosan' and 'Kangsan' cultivars was very high owing to shared crossing parents, and that of two strains from China was high, as well.

RAPD를 이용한 유연관계 분석에서는 유사도 0.37을 기준으로 2번과 8번 계통을 제외하고 21개 품종은 두 개의 그룹으로 나누어졌다. 그룹 1에는 1, 4, 5, 6, 7, 13번 계통이 분포하고, 그룹 2에는 나머지가 분포하였다.

Keywords

References

  1. Kang AS, Kong WS, Seok SJ, Hong IP, Cha DY, Kim KP, Kim DH, Yu SH. 1997. The Genetic characteristics of strains and the optimal condition for mycelial growth of Naematoloma sublateritium on sawdust media. Kor. J. Mycol. 25(2):152-160.
  2. Kim BG, Jeong MJ, Lee CS, Lee HK, Yoo YB, Ryu JC. 1995. Parameters affecting polymerase chain reaction in RAPD analysis of Pleurotus spp. Kor. J. Mycol. 23(3): 202-208.
  3. Kim JK, Lim SH, Lee DS, Chi JH, Ju YC, Seo GS, Kang HW. 2007. Genetic analysis of cultivars in Pleurotus spp. of Korea by UPR-PCR polymorphism. Kor. J. Mycol. 35(2):61-67. https://doi.org/10.4489/KJM.2007.35.2.061
  4. Huang L, Wu QP, Yang XB, Zhang JM. 2009. Taxonomic status of Pleurotus sajor-caju cultivated in China based on ITS sequence analysis. Acta Edulis Fungi 16(2):30-35.
  5. Nei M, Li WH. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA. 76:5269-5273. https://doi.org/10.1073/pnas.76.10.5269
  6. Raz V, Ecker J. 1997. DNA isolation from Arabidopsis thaliana. In: Birren B, Green E, Klapholz S,Myers R, Roskams J, eds. Genome Analysis: a Laboratory Manual, vol. 1. Newyork, USA: Cold Spring Harbor Laboratory Press. p2425.
  7. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic marker. Nucleic Acids Res. 18:6531-6535. https://doi.org/10.1093/nar/18.22.6531