Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

A Mycosis of Unidentified Fungi in Olive Flounder (Paralichthys olivaceus)

넙치의 미동정 진균 기생증

  • Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University) ;
  • Baik, Keun Sik (Department of Food Science and Technology and Functional Food Research Center, Chonnam National University) ;
  • Kim, Duwoon (Department of Food Science and Technology and Functional Food Research Center, Chonnam National University) ;
  • Seo, Joong-Kyeong (Department of Aqualife Medicine, Chonnam National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University) ;
  • Kang, So Young (Department of Aqualife Medicine, Chonnam National University)
  • 김위식 (전남대학교 수산생명의학과) ;
  • 백근식 (전남대학교 농식품생명화학부) ;
  • 김두운 (전남대학교 농식품생명화학부) ;
  • 서중경 (전남대학교 수산생명의학과) ;
  • 오명주 (전남대학교 수산생명의학과) ;
  • 강소영 (전남대학교 수산생명의학과)
  • Received : 2015.10.30
  • Accepted : 2015.12.10
  • Published : 2015.12.31
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Abstract

In 2014, high mortality was observed in olive flounder (Paralichthys olivaceus) during experimental infection. Diseased fish did not show any outward clinical signs, but numerous fungal hyphae were detected in the gills. No parasites or bacteria were isolated from the diseased fish. A high mortality rate (100%) resulted when cohabitation with fungi-infected fish were applied to healthy fish. The affected fish exhibited sever fungi infection in the gills. Histopathological examination revealed numerous fungal hyphae and mycelium around gill filament, lamellar and racker. These results suggest that the fungi may be related with the mortality of olive flounder.

본 연구에서는 2014년 넙치 치어를 사용하여 실험하는 과정에서 폐사가 발생하였다. 병어는 외관상 특이한 증상을 보이지 않았으나 아가미에서 많은 양의 균사가 관찰되었다. 기생충과 세균은 병어로부터 분리되지 않았다. 진균에 감염된 넙치와 건강한 넙치를 혼합 사육한 결과, 건강한 넙치는 7일이내에 100% 폐사되었고 폐사어는 아가미에 진균이 심하게 감염되어 있었다. 병리조직학적 검사 결과, 아가미의 새엽, 새판 및 새파 주위에 균사체와 다수의 균사가 관찰되었다. 이상의 연구 결과, 넙치의 폐사는 진균 감염과 연관성이 있을 것으로 사료되었다.

Keywords

References

  1. Egusa, S., H. Wakabayashi and K. muroga. 2006. Infectious and parasitic diseases of fish and shellfish. Life Science Publishing Co., pp. 271-296. (in Korean)
  2. Forbes, B.A., D.F. Sahm and A.S. Weissfeld. 2012. Diagnostic microbiology. Panmun education, pp. 1-18. (in Korean)
  3. Hatai, K. 2012. Diseases of fish and shellfish caused by marine fungi. Prog. Mol. Subcell. Biol., 53: 15-52. https://doi.org/10.1007/978-3-642-23342-5_2
  4. Kim, W.S., H.N. Ok, D.H. Kim, H.Y. Kim and M.J. Oh. 2011. Current status of pathogen infection in cultured eel Anguilla japonica between 2000 and 2010. J. Fish Pathol., 24: 237-245. (in Korean) https://doi.org/10.7847/jfp.2011.24.3.237
  5. Kim, W.S., M.J. Oh, S.J. Jung, Y.J. Kim and S.I. Kitamura. 2005. Characterization of an iridovirus detected from cultured turbot Scophthalmus maximus in Korea. Dis. Aquat. Org., 64: 175-180. https://doi.org/10.3354/dao064175
  6. Kurata, O., C. Munchan, S. Wada, K. Hatai, Y. Miyoshi and Y. Fukuda. 2008. Novel exophiala infection involving ulcerative skin lesions in Japanese flounder Paralichthys olivaceus. Fish Pathol., 43: 35-44. https://doi.org/10.3147/jsfp.43.35
  7. Madigan, M.T., J.M. Martinko, P.V. Dunlap and D.P. Clark. 2011. Biology of microorganisms. Twelfth Edition. Bioscience, pp. 540-544. (in Korean)
  8. Park, S.W. and M.J. Oh. 2011. Aqualife disease. Bioscience, pp. 361-401. (in Korean)
  9. Ramaiah, N. 2006. A review on fungal diseases of algae, marine fishes, shrimps and corals. Indian. J. Mar. Sci., 35: 380-387.
  10. White, T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., D.H. Gelfand, J.J. Sninsky and T.J. White (eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, pp. 315-322.