Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Prevalence of Multi-drug Resistant Bacteria Belonging to Gram Negative Enterobacteriaceae Isolated from a Domestic Stream

국내 하천에서 분리된 그람 음성 Enterobacteriacaea의 항생제 다제내성

  • Jang, Yejin (Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University) ;
  • Song, Ki-Bong (Development and Education Division, National Institute of Chemical Safety) ;
  • Chung, In-Young (Chemicals Research Division, Environmental Health Research Department, National Institute of Environmental Research Complex) ;
  • Kim, Hyuk (Chemicals Research Division, Environmental Health Research Department, National Institute of Environmental Research Complex) ;
  • Seok, Kwang-Seol (Chemicals Research Division, Environmental Health Research Department, National Institute of Environmental Research Complex) ;
  • Go, Eun Byeul (Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University) ;
  • Kim, Byeori (Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University) ;
  • Yoo, Yong-Jae (Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University) ;
  • Rhee, Ok-Jae (DK EcoV Environmental Microbiology Lab.) ;
  • Chae, Jong-Chan (Division of Biotechnology and Advanced Institute of Environmental and Bioscience, Chonbuk National University)
  • 장예진 (전북대학교 생명공학부 및 환경신기술연구소) ;
  • 송기봉 (화학물질안전원 연구개발교육과) ;
  • 정인영 (국립환경과학원 화학물질연구과) ;
  • 김혁 (국립환경과학원 화학물질연구과) ;
  • 석광설 (국립환경과학원 화학물질연구과) ;
  • 고은별 (전북대학교 생명공학부 및 환경신기술연구소) ;
  • 김벼리 (전북대학교 생명공학부 및 환경신기술연구소) ;
  • 유용재 (전북대학교 생명공학부 및 환경신기술연구소) ;
  • 이옥재 (DK EcoV 환경미생물연구소) ;
  • 채종찬 (전북대학교 생명공학부 및 환경신기술연구소)
  • Received : 2015.12.08
  • Accepted : 2015.12.09
  • Published : 2015.12.28
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Abstract

Enterobacteriaceae is one of the major families responsible for public health threats. Due to the emergence of pathogens with antibiotic resistance, great concern has been raised regarding the prevalence of antibiotic resistant bacteria in natural environments. Therefore, the diversity of Gram negative Enterobacteriaceae was investigated in water samples collected from five streams in Korea using the cultivation method. Profiling of multi-drug resistance was conducted with isolates via disk diffusion assay. The results indicated that the Gram negative Enterobacteriaceae consisted of the following genus; Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Pantoea, Plesiomonas, Raoultella, Shigella and Enterobacter. These latter strains represented 49% of identified isolates. In addition, 78.3% of the identified genus exhibited resistance against more than seven out of thirteen tested antibiotics, suggesting a high prevalence of multi-drug resistant bacteria in natural environments.

Enterobacteriaceae에 속하는 세균들은 보건학적 문제를 발생시키기도 하며 항생제 내성 병원균의 발생으로 인하여 자연환경 중의 항생제 내성균에 분포에 대한 관심이 증대되고 있다. 따라서 배양법을 이용하여 국내 5개의 하천시료로부터 그람 음성 Enterobacteriaceae의 종 다양성을 조사하였으며 분리된 세균에 대한 다제내성을 항생제디스크 확산법으로 분석하였다. 그 결과, 분리된 그람 음성 Enterobacteriaceae는 Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Pantoea, Plesiomonas, Raoultella, Shigella로 동정되었으며 49%가 Enterobacter로서 우점종으로 분석되었다. 또한 이들 세균들의 78.3%가 조사된 13종의 항생제 중에서 7종 이상에 내성을 보였으며 이것은 국내 자연하천환경에 높은 비율의 다제내성균이 서식한다는 것을 제시한다.

Keywords

References

  1. Azevedo I, Albano H, Silva J, Teixeira P. 2015. Antibiotic resistance of Enterobacteriaceae isolated from the domestic food related environments. J. Food Qual. Hazards Control 2: 51-55.
  2. Canton R, Morosini MI. 2011. Emergence and spread of antibiotic resistance following exposure to antibiotics. FEMS Microbiol. Rev. 35: 977-991. https://doi.org/10.1111/j.1574-6976.2011.00295.x
  3. Cho SH, Lim YS, Kang YH. 2012. Comparison of antimicrobial resistance in Escherichia coli strains isolated from healthy poultry and swine farm workers using antibiotics in Korea. Osong Public Health Res. Perspect 3: 151-155. https://doi.org/10.1016/j.phrp.2012.07.002
  4. Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57: 2259-2261. https://doi.org/10.1099/ijs.0.64915-0
  5. D'Costa VM, King CE, Kalan L, Morar M, Sung WW, Schwarz C, et al. 2011. Antibiotic resistance is ancient. Nature 477: 457-461. https://doi.org/10.1038/nature10388
  6. Ding C, He J. 2010. Effect of antibiotics in the environment on microbial populations. Appl. Microbiol. Biotechnol. 87: 925-941. https://doi.org/10.1007/s00253-010-2649-5
  7. Fankam AG, Kuiate JR, Kuete V. 2015. Antibacterial and antibiotic resistance modifying activity of the extracts from allanblackia gabonensis, combretum molle and gladiolus quartinianus against Gram-negative bacteria including multi-drug resistant phenotypes. BMC Complement. Altern. Med. 15: 206. https://doi.org/10.1186/s12906-015-0726-0
  8. Kang CI. 2011. Therapeutic strategy for the management of multidrug-resistant gram-negative bacterial infections. J. Korean Med. Assoc. 53: 325-331.
  9. Kim S, Yun Z, Ha UH, Lee S, Park H, Kwon EE, et al. 2014. Transfer of antibiotic resistance plasmids in pure and activiated sludge cultures in the presence of environmentally representative micro-contaminat concentrations. Sci. Total Environ. 468-469: 813-820. https://doi.org/10.1016/j.scitotenv.2013.08.100
  10. Netikul T, Kiratisin P. 2015. Genetic characterization of carbapenem- resistant Enterobacteriaceae and the spread of carbapenem- resistant Klebsiella pneumonia ST340 at a university hospital in Thailand. PLoS One 10: e0139116. https://doi.org/10.1371/journal.pone.0139116
  11. Paterson DL. 2006. Resistance in Gram-negative bacteria: Enterobacteriaceae. Am. J. Med. 119: S20-S28.
  12. Schwaiger K, Huther S, Holzel C, Kampf P, Bauer J. 2012. Prevalence of antibiotic-resistant enterobacteriaceae isolated from chicken and pork meat purchased at the slaughterhouse and at retail in Bavaria, Germany. Int. J. Food Microbiol. 154: 206-211. https://doi.org/10.1016/j.ijfoodmicro.2011.12.014
  13. Sengelov G, Agerso Y, Halling-Sorensen B, Baloda SB, Andersen JS, Jensen LB. 2003. Bacterial antibiotic resistance levels in Danish farmland as a result of treatment with pig manure slurry. Environ. Int. 28: 587-595. https://doi.org/10.1016/S0160-4120(02)00084-3
  14. Sharama R, Sharma CL, Kapoor B. 2005. Antibacterial resistance: current problems and possible solutions. Indian J. Med. Sci. 59: 120-129. https://doi.org/10.4103/0019-5359.15091
  15. Tebano G, Geneve C, Tanaka S, Grall N, Atchade E, Augustin P, et al. 2015. Epidemiology and risk factors of multidrug-resistant bacteria in respiratory samples after lung transplantation. Transpl. In. Dis. doi: 10.1111/tid.12471.
  16. Zhang X, Zhang T, Fang HH. 2009. Antibiotic resistance genes in water environment. Appl. Microbiol. Biotechnol. 82: 397-414. https://doi.org/10.1007/s00253-008-1829-z

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