Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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The Antidiabetic Effects of Black Ginseng Extract(BGE)and Geumsan Black Ginseng 05-FF(GBG05-FF) on In Vitro and In Vivo Assay

흑삼추출물(BGE)과 금산흑삼표준화소재(GBG05-FF)의 In Vitro와 In Vivo상에서의 항당뇨효과

  • Seo, Yun-Soo (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Shon, Mi-Yae (International Ginseng and Herb Research Institute) ;
  • Kong, Ryong (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kang, Ok-Hwa (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Zhou, Tian (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Do-Yeon (International Ginseng and Herb Research Institute) ;
  • Park, Jong-Dae (International Ginseng and Herb Research Institute) ;
  • Kwon, Dong-Yeul (Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University)
  • 서윤수 (원광대학교 약학대학 한약학과 한약연구소) ;
  • 손미례 ((재)금산국제인삼약초연구소) ;
  • 공룡 (원광대학교 약학대학 한약학과 한약연구소) ;
  • 강옥화 (원광대학교 약학대학 한약학과 한약연구소) ;
  • 주전 (원광대학교 약학대학 한약학과 한약연구소) ;
  • 김도연 ((재)금산국제인삼약초연구소) ;
  • 박종대 ((재)금산국제인삼약초연구소) ;
  • 권동렬 (원광대학교 약학대학 한약학과 한약연구소)
  • Received : 2015.11.30
  • Accepted : 2015.12.11
  • Published : 2015.12.31
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Abstract

The prevalence of diabetes mellitus continues to rise alarmingly because of industrialization of society. The aim of this study was to investigate the antidiabetic effects of black ginseng extract (BGE) which was performed panax ginseng. First we examined the inhibitory effects of BGE on ${\alpha}$-glucosidase. But there was no practical effects in our observations. We, also, investigate the effects of BGE on glucose uptake of skeletal muscle using 2-NBDG in $C_2C_{12}$ myotube. BGE significantly improved the glucose uptake considered as a lowered blood glucose level. Effects of GBG05-FF on fasting blood glucose and glycated hemoglobin (HbA1c) were investigated in streptozotocin (STZ)-induced diabetic mouse. After injection of STZ, fasting blood glucose and glycated hemoglobin rapidly increased. But STZ-induced diabetic mouse treated with GBG05-FF significantly reduced the level of fasting blood glucose and HbA1c. This results showed that supplementation of BGE improve the diabetic parameters and BGE have a potentiality as a functional food for Diabetes mellitus.

Keywords

References

  1. World Health Organization (1994) WHO Study group of prevention of Diabetes Mellitus. WHO Technical 5 Report Series No. 844. p. 1-100.
  2. Ahn, K. J. (2010) Westernization of Korean diabetes. Korea Clin. Diabetes 11: 91-94.
  3. Kastorini, C. M. and Panagiotakos D. B. (2009) Dietary patterns and prevention of type 2 diabetes: from research to clinical practice; a systematic review. Curr. Diabetes Rev. 5: 221-227. https://doi.org/10.2174/157339909789804341
  4. Mahendran, G., Thamotharan, G., Sengottuvelu, S. and Bai, V. N. (2014) Anti-diabetic activity of Swertia corymbosa (griseb.) Wight ex C. B. Clarke aerial parts extract in streptozotocin induced diabetic rats. J. Ethnopharmacol. 151: 1175-1183. https://doi.org/10.1016/j.jep.2013.12.032
  5. Liu, Z. H., Chen, H. G., Wu, P. F., Yao, Q., Cheng, H. K., Yu, W. and Liu, C. (2015) Flos puerariae extract ameliorates cognitive impairment in streptozotocin-induced diabetic mice. Evid. Based Complement Alternat. Med. 2015: 873243. doi: 10.1155/2015/873243.
  6. Kumkrai, P., Weeranantanapan, O. and Chudapongse, N. (2015) Antioxidant, ${\alpha}$-glucosidase inhibitory activity and subchronic toxicity of Derris reticulata extract: its antidiabetic potential. BMC Complement Altern. Med. 27: 35.
  7. Shin, W. S. (2012) Studies on characteristic compounds and physiological activities of Black Ginseng (Ginseng Radix nigra). Ph.D. thesis. Hoseo University: p. 1-166.
  8. Sun, B. S., Xu, M. Y., Li, Z., Wang, Y. B. and Sung, C. K. (2012) UPLC-Q-TOF-MS/MS analysis for steaming timesdependent profiling of steamed Panax quinquefolius and its ginsenosides transformations induced by repetitious steaming. J. Ginseng Res. 36: 277-290. https://doi.org/10.5142/jgr.2012.36.3.277
  9. Kim, H. J., Lee, J. Y., You, B. R., Kim, H. R., Choi, J. E., Nam, K. Y., Moon, B. D. and Kim, M. R. (2011) Antioxidant activities of ethanol extracts from black ginseng prepared by steaming-drying cycles. J. Korean Soc. Food Sci. Nutr. 40: 156-162. https://doi.org/10.3746/jkfn.2011.40.2.156
  10. Kim, S. J. and Kim, A. K. (2015) Anti-breast cancer activity of fine black ginseng (Panax ginseng Meyer) and ginsenoside Rg5. J. Ginseng Res. 39: 125-134. https://doi.org/10.1016/j.jgr.2014.09.003
  11. Lee, M. R., Kim, B. C., Kim, R., Oh, H. I., Kim, H. K., Choi, K. J. and Sung, C. K. (2013) Anti-obesity effects of black ginseng extract in high fat diet-fed mice. J. Ginseng Res. 37: 308-349. https://doi.org/10.5142/jgr.2013.37.308
  12. Park, H. J., Shim, H. S., Kim, K. S. and Shim, I. (2011) The protective effect of black ginseng against transient focal ischemia-induced neuronal damage in rats. Korean J. Physiol. Pharmacol. 15: 333-338. https://doi.org/10.4196/kjpp.2011.15.6.333
  13. Zhang, L., Hogan, S., Li, J., Sun, S., Canning, C., Zheng, S. J. and Zhou, K. (2011) Grape skin extract inhibits mammalian intestinal ${\alpha}$-glucosidase activity and suppresses postprandial glycemic response in streptozocin-treated mice. Food Chem. 126: 466-471. https://doi.org/10.1016/j.foodchem.2010.11.016
  14. Jung, D. W., Ha, H. H., Zheng, X., Chang, Y. T. and Williams, D. R. (2011) Novel use of fluorescent glucose analogues to identify a new class of triazine-based insulin mimetics possessing useful secondary effects. Mol. Biosyst. 7: 346-358. https://doi.org/10.1039/C0MB00089B
  15. Kim, J. Y., Bae, H. Y., Park, S. Y., Kim, Y. W. and Lee, S. G. (1998) Effects of regular treadmill running on GLUT4 protein of skeletal muscle in STZ-diabetic rats. The Yeungnam Univ. Med. J. 15: 341-349. https://doi.org/10.12701/yujm.1998.15.2.341
  16. Lee, J. S., Son, H. S., Maeng, Y. S., Chang, Y. K. and Ju J. S. (1994) Effects of buckwheat on organ weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats. Korean J. Nutrition 27: 819-827.
  17. International Expert Committee (2009) International expert committee report on the role of the A1C assay in the diagnosis of diabetes. Diabetes Care 32: 1327-1334. https://doi.org/10.2337/dc09-9033