Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Reproduction and Parasitization Capacity of an Insect Parasitic Mite, Pyemotes moseri Yu et Liang (Acarina: Pyemotidae) New to Korea

국내 미기록 곤충기생성 응애, Pyemotes moseri Yu et Liang (Acarina: Pyemotidae)의 생식 및 기생 능력

  • Kim, Se-Jin (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Jong Ho (Honam Regional Branch, Animal and Plant Quarantine Agency (QIA), MIFAFF) ;
  • Yang, Chang Yeol (National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA)) ;
  • Kang, Taek-Jun (National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA)) ;
  • Cho, Myoung Rae (Technology Cooperation Bureau, Rural Development Administration) ;
  • Hong, Ki-Jeong (Department of Plant Medicine, Sunchon National University)
  • 김세진 (서울대학교 농생명공학부) ;
  • 이종호 (농림축산검역본부 호남지역본부) ;
  • 양창열 (농촌진흥청 국립원예특작과학원) ;
  • 강택준 (농촌진흥청 국립원예특작과학원) ;
  • 조명래 (농촌진흥청 기술협력국) ;
  • 홍기정 (순천대학교 식물의학과)
  • Received : 2015.10.13
  • Accepted : 2015.11.04
  • Published : 2015.12.01
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Abstract

An insect parasitic mite was found on a larva from Japanese apricot seed. The mite was identified as Pyemotes moseri Yu et Liang (Acarina: Pyemotidae) new to Korea. The host larva was identified as Eurytoma maslovskii using mitochondrial DNA sequencing analysis. We conducted preliminary study on its reproduction and parasitization capacity in laboratory condition. A mated female mite reared on Eurytoma maslovskii larva. We counted and sexed newborn progenies and then eliminated them during periodical observations. To test parasitization capacity, a PCR tube containing mass reared P. moseri and Japanese apricot seeds (assumed bear larva of E. maslovskii) placed in a stainless bath filled with potting soil. One month later, we surveyed the seeds whether the E. maslovskii larva parasitized by mite or not. We repeated this experiment three times with five replications each. Average life span (days from parasitization to the end of reproduction) of gravid females was 24.4 days (n=8). A gravid female reproduced 104.0 female progenies (n=8). Although there were more than seven Japanese apricot seeds per bath containing larva or pupa, we found parasitization only in two seeds.

국내 미기록종 Pyemotes moseri Yu et Liang (Acarina: Pyemotidae)는 곤충기생성 응애로 매실 씨앗 속 유충에서 처음 발견되었다. 발견당시 응애가 기생하고 있었던 기주의 미토콘드리아 유전자를 증폭하여 염기서열을 분석한 결과 복숭아씨살이좀벌의 유충으로 밝혀졌다. 본 연구는 실험실 조건하에서 P. moseri의 번식과 기생 능력을 조사하였다. 복숭아씨살이좀벌의 유충을 기주로 이용하여 P. moseri의 교미한 암컷 성충을 사육하면서 주기적으로 관찰하여 새로 태어나는 자손의 수와 성별을 조사한 후 제거하였다. 기생 능력 조사는 원예용 상토가 깔린 스테인레스 바트에 대량 사육한 응애가 들어 있는 튜브와 복숭아씨살이좀벌 유충이 가해한 매실 씨앗을 함께 담은 후 지퍼백에 넣어 인큐베이터에 보관하였다. 복숭아씨살이좀벌 유충 또는 번데기에 응애의 기생 여부를 확인하고자 한달 후 매실 씨앗을 조사하였으며 본 실험은 5반복씩 3회 실시하였다. 교미한 암컷이 기생하기 시작한 이후부터 생식이 끝날 때까지의 기간은 평균 24.4일(n=8)이었으며 교미한 암컷 한 마리당 평균 104.0마리(n=8)의 암컷을 낳았디. 복숭아씨살이좀벌의 유충 또는 번데기가 들어 있는 매실 씨앗은 바트당 평균 7개 이상이었고 이중 단 2개의 씨앗에서만 기생에 성공한 응애를 관찰하였다.

Keywords

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