Nutrition Research and Practice

  • 제9권2호
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  • Pages.123-128
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  • 2015
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  • 1976-1457(pISSN)
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  • 2005-6168(eISSN)
한국영양학회 (The Korean Nutrition Society)
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Protective role of oligonol from oxidative stress-induced inflammation in C6 glial cell

  • Ahn, Jae Hyun (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Ji Won (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Ji Myung (Department of Food Science and Nutrition, Pusan National University) ;
  • Maeda, Takahiro (Amino Up Chemical Co., Ltd.) ;
  • Fujii, Hajime (Amino Up Chemical Co., Ltd.) ;
  • Yokozawa, Takako (Institute of Natural Medicine, University of Toyama) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
  • 투고 : 2014.05.26
  • 심사 : 2014.08.27
  • 발행 : 2015.04.01
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초록

BACKGROUND/OBJECTIVES: Natural products or active components with a protective effect against oxidative stress have attracted significant attention for prevention and treatment of degenerative disease. Oligonol is a low molecular weight polyphenol containing catechin-type monomers and oligomers derived from Litchi chinensis Sonn. We investigated the protective effect and its related mechanism of oligonol against oxidative stress. MATERIALS/METHODS: Oxidative stress in C6 glial cells was induced by hydrogen peroxide ($H_2O_2$) and the protective effects of oligonol on cell viability, nitric oxide (NO) and reactive oxygen species (ROS) synthesis, and mRNA expression related to oxidative stress were determined. RESULTS: Treatment with oligonol inhibited NO and ROS formation under cellular oxidative stress in C6 glial cells. In addition, it recovered cell viability in a dose dependent-manner. Treatment with oligonol also resulted in down-regulated mRNA expression related to oxidative stress, nuclear factor kappa-B (NF-${\kappa}B$) p65, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS), compared with the control group treated with $H_2O_2$. In particular, expression of NF-${\kappa}B$ p65, COX-2, and iNOS was effectively reduced to the normal level by treatment with $10{\mu}g/mL$ and $25{\mu}g/mL$ of oligonol. CONCLUSIONS: These results indicate that oligonol has protective activity against oxidative stress-induced inflammation. Oligonol might be a promising agent for treatment of degenerative diseases through inhibition of ROS formation and NF-${\kappa}B$ pathway gene expression.

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참고문헌

  1. Coyle JT, Puttfarcken P. Oxidative stress, glutamate, and neurodegenerative disorders. Science 1993;262:689-95. https://doi.org/10.1126/science.7901908
  2. Tuppo EE, Arias HR. The role of inflammation in Alzheimer's disease. Int J Biochem Cell Biol 2005;37:289-305. https://doi.org/10.1016/j.biocel.2004.07.009
  3. Liu B, Hong JS. Role of microglia in inflammation-mediated neurodegenerative diseases: mechanisms and strategies for therapeutic intervention. J Pharmacol Exp Ther 2003;304:1-7. https://doi.org/10.1124/jpet.102.035048
  4. Cirino G, Distrutti E, Wallace JL. Nitric oxide and inflammation. Inflamm Allergy Drug Targets 2006;5:115-9. https://doi.org/10.2174/187152806776383143
  5. Pahan K, Raymond JR, Singh I. Inhibition of phosphatidylinositol 3-kinase induces nitric-oxide synthase in lipopolysaccharide- or cytokine-stimulated C6 glial cells. J Biol Chem 1999;274:7528-36. https://doi.org/10.1074/jbc.274.11.7528
  6. Jiang G, Lin S, Wen L, Jiang Y, Zhao M, Chen F, Prasad KN, Duan X, Yang B. Identification of a novel phenolic compound in litchi (Litchi chinensis Sonn.) pericarp and bioactivity evaluation. Food Chem 2013;136:563-8. https://doi.org/10.1016/j.foodchem.2012.08.089
  7. Wang L, Lou G, Ma Z, Liu X. Chemical constituents with antioxidant activities from litchi (Litchi chinensis Sonn.) seeds. Food Chem 2011;126:1081-7. https://doi.org/10.1016/j.foodchem.2010.11.133
  8. Ong PK, Acree TE. Similarities in the aroma chemistry of Gewurztraminer variety wines and lychee (Litchi chinesis sonn.) fruit. J Agric Food Chem 1999;47:665-70. https://doi.org/10.1021/jf980452j
  9. Yang B, Wang J, Zhao M, Liu Y, Wang W, Jiang Y. Identification of polysaccharides from pericarp tissues of litchi (Litchi chinensis Sonn.) fruit in relation to their antioxidant activities. Carbohydr Res 2006;341:634-8. https://doi.org/10.1016/j.carres.2006.01.004
  10. Bhoopat L, Srichairatanakool S, Kanjanapothi D, Taesotikul T, Thananchai H, Bhoopat T. Hepatoprotective effects of lychee (Litchi chinensis Sonn.): a combination of antioxidant and anti-apoptotic activities. J Ethnopharmacol 2011;136:55-66. https://doi.org/10.1016/j.jep.2011.03.061
  11. Sarni-Manchado P, Le Roux E, Le Guerneve C, Lozano Y, Cheynier V. Phenolic composition of litchi fruit pericarp. J Agric Food Chem 2000;48:5995-6002. https://doi.org/10.1021/jf000815r
  12. Fujii H, Nishioka H, Wakame K, Magnuson BA, Roberts A. Acute, subchronic and genotoxicity studies conducted with Oligonol, an oligomerized polyphenol formulated from lychee and green tea extracts. Food Chem Toxicol 2008;46:3553-62. https://doi.org/10.1016/j.fct.2008.06.005
  13. Noh JS, Park CH, Yokozawa T. Treatment with oligonol, a low-molecular polyphenol derived from lychee fruit, attenuates diabetes-induced hepatic damage through regulation of oxidative stress and lipid metabolism. Br J Nutr 2011;106:1013-22. https://doi.org/10.1017/S0007114511001322
  14. Kundu JK, Chang EJ, Fujii H, Sun B, Surh YJ. Oligonol inhibits UVB-induced COX-2 expression in HR-1 hairless mouse skin--AP-1 and C/EBP as potential upstream targets. Photochem Photobiol 2008;84:399-406. https://doi.org/10.1111/j.1751-1097.2007.00277.x
  15. Zhang XH, Yokoo H, Nishioka H, Fujii H, Matsuda N, Hayashi T, Hattori Y. Beneficial effect of the oligomerized polyphenol oligonol on high glucose-induced changes in eNOS phosphorylation and dephosphorylation in endothelial cells. Br J Pharmacol 2010;159: 928-38. https://doi.org/10.1111/j.1476-5381.2009.00594.x
  16. Ialenti A, Ianaro A, Moncada S, Di Rosa M. Modulation of acute inflammation by endogenous nitric oxide. Eur J Pharmacol 1992; 211:177-82. https://doi.org/10.1016/0014-2999(92)90526-A
  17. Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, Lee SS. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-${\kappa}$B activation. Mutat Res 2001;480-481:243-68. https://doi.org/10.1016/S0027-5107(01)00183-X
  18. Dobashi K, Pahan K, Chahal A, Singh I. Modulation of endogenous antioxidant enzymes by nitric oxide in rat C6 glial cells. J Neurochem 1997;68:1896-903.
  19. Garcion E, Sindji L, Montero-Menei C, Andre C, Brachet P, Darcy F. Expression of inducible nitric oxide synthase during rat brain inflammation: regulation by 1,25-dihydroxyvitamin D3. Glia 1998;22: 282-94. https://doi.org/10.1002/(SICI)1098-1136(199803)22:3<282::AID-GLIA7>3.0.CO;2-7
  20. Syapin PJ, Militante JD, Garrett DK, Ren L. Cytokine-induced iNOS expression in C6 glial cells: transcriptional inhibition by ethanol. J Pharmacol Exp Ther 2001;298:744-52.
  21. Peterson LJ, Flood PM. Oxidative stress and microglial cells in Parkinson's disease. Mediators Inflamm 2012;2012:401264.
  22. McCafferty DM, Mudgett JS, Swain MG, Kubes P. Inducible nitric oxide synthase plays a critical role in resolving intestinal inflammation. Gastroenterology 1997;112:1022-7. https://doi.org/10.1053/gast.1997.v112.pm9041266
  23. Aktan F. iNOS-mediated nitric oxide production and its regulation. Life Sci 2004;75:639-53. https://doi.org/10.1016/j.lfs.2003.10.042
  24. Grandison L, Nolan GP, Pfaff DW. Activation of the transcription factor NF-${\kappa}$B in GH3 pituitary cells. Mol Cell Endocrinol 1994;106: 9-15. https://doi.org/10.1016/0303-7207(94)90180-5
  25. O'Neill LA, Kaltschmidt C. NF-${\kappa}$B: a crucial transcription factor for glial and neuronal cell function. Trends Neurosci 1997;20:252-8. https://doi.org/10.1016/S0166-2236(96)01035-1
  26. Gookin JL, Chiang S, Allen J, Armstrong MU, Stauffer SH, Finnegan C, Murtaugh MP. NF-${\kappa}$B-mediated expression of iNOS promotes epithelial defense against infection by Cryptosporidium parvum in neonatal piglets. Am J Physiol Gastrointest Liver Physiol 2006;290: G164-74. https://doi.org/10.1152/ajpgi.00460.2004
  27. Korhonen R, Lahti A, Kankaanranta H, Moilanen E. Nitric oxide production and signaling in inflammation. Curr Drug Targets Inflamm Allergy 2005;4:471-9. https://doi.org/10.2174/1568010054526359
  28. Kubes P, McCafferty DM. Nitric oxide and intestinal inflammation. Am J Med 2000;109:150-8. https://doi.org/10.1016/S0002-9343(00)00480-0
  29. Kidd BL, Urban LA. Mechanisms of inflammatory pain. Br J Anaesth 2001;87:3-11. https://doi.org/10.1093/bja/87.1.3
  30. Yum HW, Zhong X, Park J, Na HK, Kim N, Lee HS, Surh YJ. Oligonol inhibits dextran sulfate sodium-induced colitis and colonic adenoma formation in mice. Antioxid Redox Signal 2013;19:102-14. https://doi.org/10.1089/ars.2012.4626
  31. Noh JS, Kim HY, Park CH, Fujii H, Yokozawa T. Hypolipidaemic and antioxidative effects of oligonol, a low-molecular-weight polyphenol derived from lychee fruit, on renal damage in type 2 diabetic mice. Br J Nutr 2010;104:1120-8. https://doi.org/10.1017/S0007114510001819

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